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III For constructing the expression vector of a fusion protein and obtain a target protein with full identity on aa sequence of a natural 13- 1,3-1 ,4-glucanase, with the recombined plasmid DNA harbouring the target gene as template, the primers designed with restriction sites for both terminals and enterokinase recognition site, followed by PCR amplification, was induced to the target gene.

为构建融合蛋白表达载体和获得与天然蛋白质序列完全一致的目的蛋白，以含有目的基因的重组质粒DNA为模板，设计引物时加入两端酶切位点及肠激酶裂解位点，通过PCR扩增引入目的基因中，测序结果表明接头和读框正确。

This paper de-duces the equation of scroll case surface with the enveloping theory,builds the envelope sur-face of ellipsoides with variable radius by using the the method of constructing an ellipse withseveral arcs and presents a new and simple method fOr geometry moulding of scroll case sur-face.

蜗壳是水电站的重要部件之一，本文以包络理论推导了蜗壳曲面方程；并采用分段圆弧构造椭圆的方法构造了变径椭球包络曲面，为蜗壳曲面的几何造型提出了一种新的简便方法。

On the basis of analyzing the formation anddevelopment of due procedure,and commenting on western jurists andphilosopher'due procedure theory,the author brings forwards his own viewon the theory of rule by law contained in due procedure,on the light of which,after expounding the relationship between due procedure and human rightsprotection、power restriction as well as constitutional authority,the authormakes a primary envisagement on constructing Chinese due process of law.

文章在分析正当程序形成、发展的历史过程和评述西方法哲学家关于正当法律程序的理论的基础上，对正当程序包含的法治原理提出了自己的观点，并以此为依据，探讨了正当程序与人权保障、权力制约、宪法权威的关系，对构建中国的正当程序作出了初步设想。

Furthermore,the synthesized compounds were used for constructing energy transfer systems with eosin and their fluorescence enhancement to eosin was studied.

进一步用其与曙红组成能量转移体系，研究了产物对曙红的荧光增强作用。

A massspring-damp model is proposed through constructing the mapping relation between equivalent parameters and finger seal＇s structural parameters.

通过建立当量模型中各等效参数和实际指尖密封结构参数之间的映射关系，构建了指尖密封迟滞性能优化的质量一弹簧一阻尼当量模型。

This method combines the information of output tracking error with the parameter estimate error in constructing the adaptation law, which gives a better result in parameter estimation than that from the traditional ARC.

该方法同时采用了输出误差与参数估计误差的相关信息构造自适应律，具有比常规ARC更好的参数估计效果。

But there are other and simpler objections to ether than the difficulty of constructing it.

把以太说成是胶状的物质已经很困难了，但是还有其他更简单的反对它的理由。

In an experiment of constructing a phylogeny of the Eutherian orders, its results not only support the Rodents,(Ferungulates, Primates grouping, but also give an evolutionary tree that is identical to the one constructed by the maximum likelihood method, which indicates that sequence distance defined is very effective to measure the relatedness between sequences.

在研究基于基因组序列的进化关系的实验中，不仅支持了哺乳类动物中（啮齿动物，（灵长类动物，Ferungulates））进化关系分类的结论，而且构造的进化树也与由最大似然方法构造的进化树完全一致。这充分说明了我们给出的序列距离对度量序列相似性是非常有效的。

This paper states the everglade actuality in Daxing'anling district,points out the relationship between protecting and utilizing everglade and exerting its ecology functions,puts forward that the protection and utilization of everglade are necessary for ensuring national ecology safety,realizing ecology harmonious and constructing socialism forest area.

对大兴安岭湿地的现状进行了较为全面的论述，阐述了保护和利用大兴安岭湿地与发挥大兴安岭生态功能的关系，提出了湿地的保护和利用是保障国家生态安全的需要、是实现生态和谐的必由之路、是建设社会主义和谐林区的有力保障。

For those operators that are willing to allow RMT to take place, constructing their own Station Exchange could be a solution.

那些允许虚拟交易的运营商，则可以建立他们自己的Station Exchange（索尼在线娱乐所创虚拟交易平台）。

If you are committed to an education career, then the Master of Arts in Education from Ashford University is for you.

如果你是致力于教育事业，那么，艺术教育硕士学位阿什福德大学是给你的。

objective:to explore the expression of survivin protein and its mrna in rectal cancer and their clinic significance.

目的：研究凋亡抑制因子survivin蛋白及其mrna在直肠癌组织中的表达及其与临床病理关系。