查询词典 construct

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

When a chance offers itself, they are quite ready to expropriate the possessors, but they neither want nor are able to construct a new social order.

当有适当的机会时，他们非常有可能没收有产者的财产，但是他们既不希望也没有能力建立社会新秩序。

The basic principle of writing therapy includes decrease perception of cause and effect relations and insight;construct alternative story through integration and reconstruction of events;and externalize problems.

写作治疗的基本原理包括：降低压力经验的通达性，减少闯入记忆；形成叙事连贯，达到因果认识与领悟；对事件整合和重构，建构另种生活故事；把问题外化。

To formulate an accounting standard is not away from its theoretic foundation, theory research on financial reporting from effectiveness perspective builds the substantially theoretic foundation in order to design the financial reporting standard in extractive coal industry, so to construct the theory frame on financial reporting from effectiveness perspective is another purpose.

目前，国内外关于石油天然气行业会计信息披露已发布了具体会计准则，但它不适用于煤炭等其他矿产资源；尽管国际上关于矿产资源的勘探与评价已发布了国际财务报告准则，但它只适用于生产程序的某一阶段；煤炭采掘业会计信息披露没有自己的行业规范，设计《企业会计准则——煤炭采掘业生产活动》是本文研究的重要目的。

In this thesis, based on the fuzzy extractor, we construct a fingerprint authentication system.

在这一篇论文中，我们以模糊撷取器为基础建构一个指纹认证系统。

It is impossible to logically infer the classically expressed geodesic differential equation from the ontology differential geometry. Besides, the initial proposition how to construct a functional extreme value problem for the shortest curve on a surface, which was supposed first by Gauss, would be an improper formal expression with a wrong premise.

不仅仅无法由"实体论"微分几何逻辑地推导出经典的测地线方程，而且Gauss曾经为"曲面上最短线"问题所构造的泛函极值命题，实质上属于一个"前提认定"完全错误的不当表述。

Anyway,such a research route may lead to another extremeness,which put all of the efforts on merchants\' own efforts to construct commercial order,which too much emphasized civil regulations\' function and role.

但是这样的研究思路也易导致另一个极端，就是只把目光投放到商人自身在构建商业秩序方面的努力，过分强调了民间规范的功能和作用。

The experiment objects are 15 age-related cataract patients (28 target eyes). Furthermore, this study makes an Age-related Cataract Image Blurring Program which can blur the digital eye chart for the second experiment -"Blurry Image Recognition Experiment". The purpose of the second experiment is to decrease the visual acuity of young healthy eyes (vision with correction, 1.0) and to gather statistics for recognition critical point. These statistics represent different blurry degree of visual degeneration and are used to construct the simulation function of testbed.

透过本研究撰写之老年性白内障影像模糊化程式，将电子式视力检查表进行模糊影像处理，并由视觉屈光正常之受测者(或矫正视力为1.0之受测者)进行模糊影像辨识实验，完成每行视标之可辨识临界点之统计，再将统计数据应用於平台制作，以模拟不同视力退化程度之功能。

Pyramid: Ancient monumental structure construct ed of or faced with stone or brick and having a rectangular base and four sloping triangular sides meeting at an apex.

金字塔：用砖、石材料建造或表面覆以砖、石的古代纪念性建筑物，地基为矩形，四面为倾斜的三角形在顶端交会。

With these conclusions, we can construct a motivational factor model of knowledge workers in high-tech enterprises.

根据这些研究成果，我们可以构建出知识型员工激励因素模型，从而为企业设计和制订知识型员工的激励政策提供有力的参考。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。