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This study was aimed to construct a lentiviral vector carrying human coagulant factor Ⅷ and to investigate its expression in 293T cells.

本研究目的是构建含有人凝血因子Ⅷ基因的慢病毒载体，观察其在293T细胞中的表达情况。

How to construct good codes is a basic problem in coding theory.

构造好码是编码理论的一个基本问题。

These two types construct the basis of codon usage on the function and expression stage, respectively.

痘苗病毒以基因的功能构筑密码子使用类型的基础，而腺病毒则以表达的时相构筑密码子使用类型的基础。

A new method based on subtractive image segmentation for weighted image fusion is put forward from another viewpoint. It adopts the improved particle swarm optimization to calculating the segmentation threshold of subtractive image and the corresponding weighted fusion coefficient in space domain or in wavelet decomposition domain. Moreover, it doesnt need to construct the eigenspace and quantitative eigenvalue for the weighted fusion coefficients.

本文从另外一个角度提出了一种基于差值图像分割的加权图像融合方法，它采用改进的粒子群优化方法在空间域或小波分解域求解差值图像的分割阈值和对应的加权融合系数，而不需要为计算融合系数而构造特征空间和定量特征关系。

The article think, from the point of morality, its an imperative and realistic matter that how to cogitate the moral importance and problems of gene technology rationally, and construct relevant system of morality on the condition of gene technology develops quickly.

本文认为，从伦理学的角度来看，如何在基因技术飞速发展的形势下，理性地认识基因技术的伦理意义及可能引起的伦理问题，并建构相应的伦理体系是个迫切而又现实的问题。

This approach will help you construct a cohesive message.

这种方法可以保证你给出的信息连贯。

In section 7,we give a way to construct even dimensional symmetric self-dual Liealgebras from the point of view of cohomology.

在最后一节中，我们从上同调的观点，给出了偶数维对称自对偶李代数的一种构造方法。

To construct recombinant adenovirus,the N and G genes were respectively cleaved from the pMD18-T plasmids by double restriction endonuclease,and then cloned into shuttle vector pAdTrack-CMV coinstantaneously.

经卡那霉素抗性和酶切筛选，最终成功构建了含有N基因和G基因的重组穿梭载体pAdTrack-CMV-N-G。

If no cold frame is available, you can construct a make-shift cold frame using hay or straw bales placed to form a square with plastic sheeting anchored over the square.

如果没有冷框，您可以建造一个改装的冷框，使用干草或秸秆放成一个方块形，用塑料布放置在上面。

Although Construction craft consummates day by day,for improper craft and The construct control not standard,the quality accident such as breaking the pile.stoping up the tube ,clamping the putty,the honey comb,less filling and so on occurs sometimes.

目前，在桩基施工中，质量控制能力较差，控制手段不力，造成质量水平不稳定，重大工程质量事故时有发生，给国家、人民的财产带来巨大损失。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐