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In order to construct a normalized cDNA silencing library, a novel procedure was adopted to reduce the abundant copy number and increase the rare copy number. Then the normalized cDNA was put into pTRV vector to construct a cDNA library and this cDNA library was screened with the technology of virus induced gene silencing.

然后把均一化之后mRNA反转录得到cDNA，再与基因沉默载体pTRV重组，最后把构建好的载体通过电转化的方法转入到GV3101农杆菌中，从而建立起破色期番茄果实均一化cDNA沉默文库。

However, the strategy and rhetoric that these Puritans employed to construct their American identity were replicas of those employed by the Anglicans in their effort to construct an identity for the English as God's chosen people in a fight against the Antichristian influence of the Roman Catholic Church.

然而清教徒移民建构认同的策略，实系挪用自十六、十七世纪时英国新教徒建构自我与英国国家认同的策略，只不过不同於移民美洲的清教徒，英国新教徒自视为上帝的选民，反基督的他者则是罗马天主教。

He supports continuously with help artists, among them include since a few centuries biggest Christianity church- big bema of Peter of saint design bright 基 of rice with Italian painter with construct the house pulls the 菲 the 尔, they once is appointed design with the glorious mural that construct a the 著 of world big church in Vatican west 斯廷 in Rome insides.

他不断支持和帮助艺术家们，其中包括几个世纪以来最大的基督教教堂----圣彼得大教堂的设计者米开朗基罗和意大利画家和建筑家拉菲尔，他们曾被委任设计和建造世界著名的罗马梵蒂冈西斯廷大教堂内的辉煌壁画。

It analyses the current situation of the offer booklist information construct by China, put forward the offer booklist information of China which make the person of the book supply construct.

通过对我国可供书目信息建设现状的分析，提出我国的可供书目信息网应由图书供应商建设，行业共享。

The thesis studies the difference between GWSs composition and traditional workflow applications, proposes the implement mechanism and technical architecture of GWSs composition and defines the reference model of GWSs composition platform, which provide holistic framework and top-level guidance for studying dynamic GWSs composition technology, designing GWSs composition platform, and implementing representative composite service applications.2、Theories and methods of composite process modeling and process analysis. This thesis proposes a meta-model of Geo-spatial Web services composition process defining from two aspects, i.e. process and resource. According to the process definition meta-model, we propose a new Geo-spatial Web services composition Process/Resource model based on extended WF-net, which effectively resolves the problems on uncertain activity option and dynamic variation of service in composite process model. A notion with regard to soundness of process model is proposed from three aspects, i.e. the structural correctness of process model, validity of data link and validity of resource. The notion of soundness definitely gives a dividing line on correctness of composite GWS process. This thesis analyzes sufficient and essential terms of soundness, and studies compositional property of WSCP/R-net models which provides an effective method to construct a soundness WSCP/R-net model. According to WSCP/R-net, the thesis proposes composition algebra and studies the properties of it. Based on the composition algebra, 6 kinds of composite process reduction rules are induced to optimize the process.3、Geo-spatial Web service QoS model and its application architecture. This thesis proposes an extendable GWS QoS model from three aspects, i.e. Geo-spatial Web Service itself, networking conditions and service consumer which can be used to distinguish GWSs with overlapping or identical functionality. We work over the QoS evaluation methods of composite GWS process which can be used to guide the execution, monitor and service selection of composition process. Aming at some disadvantages in traditional Web service discovery model for its lacking of QoS supporting, this thesis proposes a new service discovery model SDMQ supporting QoS constraints.4、Technologies of composite service executing. SSPL, a new composite service process language, is defined to overcome the disadvantages of existing composite services process languages which can not adapt to the dynamic variation of Geo-spatial Web Services. An algorithm is described to translate WSCP/R-net model into SSPL. The thesis studies the model of dynamic services selection with QoS global optimization, presenting an algorithm GODSS to resolve dynamic services selection with QoS global optimization in GWSs Composition.5、Design and implement experimental system and representative applications. According to the research findings presented above, we design and implement an experimental system and construct two representative applications to show that our achievements are effective and practical.

针对当前空间信息服务聚合研究中存在的若干不足，本文重点在基于工作流的空间信息服务聚合框架、空间信息服务聚合流程建模和分析技术、空间信息服务QoS模型及应用体系以及空间信息服务聚合流程执行等几个方面进行深入研究，主要工作和创新点包括：1、基于工作流的空间信息服务动态聚合框架：研究了空间信息服务动态聚合和传统工作流应用的不同，在此基础上提出了基于工作流技术的空间信息服务动态聚合实现机制、技术体系和参考模型，为研究空间信息服务聚合的关键技术、开发服务聚合支撑平台以及在此基础上构造特定的空间信息服务聚合应用提供了总体框架和顶层指导。2、空间信息服务聚合流程建模和分析技术：首先基于动态服务聚合流程建模的需求和空间信息服务动态变化的应用实际，从服务聚合的过程维和资源维出发定义了服务聚合流程定义元模型；与之相对应，对基本的WF-net进行扩展，提出服务聚合流程/资源网作为空间信息服务聚合流程的形式化描述模型，有效解决了现有的基于基本Petri网和工作流网的服务聚合建模方法所不能解决的不确定路径选择和服务的动态变化性问题；从流程结构正确性、数据依赖有效性和资源实现有效性三个方面提出了WSCP/R-net健壮性的概念，明确界定了聚合流程正确性的标准，并对WSCP/R-net模型的健壮性分析方法和WSCP/R-net模型的组合特性进行了研究；提出了空间信息服务聚合代数算子并对其性质进行了研究，在此基础上提出了6种聚合流程约简规则，从而可达到优化聚合流程、提高聚合流程执行效率的目的。3、空间信息服务QoS模型和应用体系：从服务本身、网络环境和服务消费者三个层次出发，提出了一个可扩展的空间信息服务QoS模型GSQM，实现了对空间信息服务的度量和评价，并对GSQM不同质量要素信息的客观、公正采集方法进行了研究；研究了空间信息服务聚合流程QoS评价方法，有效支持了空间信息服务聚合流程的执行、监控以及服务选择等操作；针对目前的服务发现模型仅支持服务功能性描述、不能有效满足空间信息服务应用需求的现状，提出了一种新的支持QoS约束的服务发现模型SDMQ，并对模型的实现框架进行了研究。4、空间信息服务聚合流程执行技术：针对现有的服务聚合流程描述语言不能有效满足空间信息服务动态聚合流程描述的特点，基于BPEL4WS提出了一种新的空间信息服务聚合流程描述语言SSPL；研究了满足健壮性要求的WSCP/R-net模型向SSPL的转换算法，在此基础上可以满足用户无显式编码来实现空间信息服务聚合应用的目的；研究了服务动态选择QoS全局优化模型，并在此基础上提出了一种解决服务动态选择QoS全局优化问题的实现算法GODSS.5、基于论文研究成果，设计实现了"空间信息服务聚合实验系统"，并构建了"矿产资源评价"和"城市消防应急响应"两个典型应用案例，对论文所述模型、方法的可行性和有效性进行了验证。

In this paper we introduce the hyperbolic cosine function and construct the new hyperbolic cosine penalty function and algorithms, further more, we construct the new hyperbolic cosine multipier penalty function .

本文在传统形式的罚函数基础上引入双曲余弦函数做罚项，构造了新的对于一般约束优化问题的双曲余弦罚函数和求解迭代公式；进一步地，又提出了求解具有等式约束优化问题的双曲罚函数乘子法。

To construct perfect charity mechanism is complement to the system of social security and also the insides need to construct the socialism diapason society.

建构完善的慈善机制，无疑是对社会保障体系的补充和完善，也是构建社会主义和谐社会的内在需求。

It solves the problem that the unitary contour presentation can not correctly extract face contour in a face image which suffers from scale, rotation etc. The definition of the internal and external energy function is provided. At the same time, the global matching algorithm and local matching algorithm is given. The experiment shows that this presentation and the accompanying matching algorithm can be used to extract the face contour very well. So the image segmentation can be implemented by using it.②By analyzing the recognition principle of PCA method, we can conclude that the face images coming from different surrounding consist of different face image space. This is the essential reason that makes the generality of PCA method worse. Also, we give a measurement means to measure the distance from different face image space, so we can analyze face image space more conveniently.③We also construct various scale models and rotation pose models to detect the scale and rotating angle of face image to be recognized. The experiment results show that the detecting precision is very high. So it is good for face image feature extraction and face image representation.④Similarly, we construct local feature models of face image and utilize them to detect the local feature of face image. At the same time, we put forward a novel face image local feature detection algorithm, locating step by step. The experiment results show that this method can accurately detect the location of local face feature in a image.⑤A novel face image presentation model, dual attribute graph , is put forward. Firstly, it utilizes attribute graph to present the face image, then exact the local principal component coefficient and Gabor transform coefficient of thc pixels which corresponds to the nodes of the graph as the attribute of the nodes. This representation fully makes use of the statistical characteristic of the local face feature and utilizes Gabor transform to present the topographical structure of face image. So DAG has more general property.⑥Based on the DAG presentation, we give a DAG matching function and matching algorithm. During the design of the function and algorithm, the noise factor, e. g., lighting, scale and rotation pose are considered and tried to be eliminated. So the algorithm can give more general property.⑦A general face image recognition system is implemented. The experiment show the system can get better recognition performance under the noise surrounding of lighting, scale and rotation pose.

本文在上述研究的基础上，取得了如下主要研究成果：①构造了一个通用的人脸轮廓模型表示，解决了由于人脸图象尺度、旋转等因素而使得仅用单一轮廓表示无法正确提取人脸轮廓的问题，并给出了模型内、外能函数的定义，同时给出了模型的全局与局部匹配算法，实验表明，使用这种表示形式以及匹配算法，能够较好地提取人脸图象的轮廓，可实际用于人脸图象的分割；②深入分析了PCA方法的识别机制，得出不同成象条件下的人脸图象构成不同的人脸图象空间的结论，同时指出这也是造成PCA方法通用性较差的本质原因，并给出了不同人脸空间距离的一种度量方法，使用该度量方法能够直观地对人脸图象空间进行分析；③构造了各种尺度模板、旋转姿势模板以用于探测待识人脸图象的尺度、旋转角度，实验结果表明，探测精确度很高，从而有利于人脸图象特征提取，以及图象的有效表示；④构造了人脸图象的各局部特征模板，用于人脸图象局部特征的探测；同时提出了一种新的人脸图象局部特征探测法---逐步求精定位法，实验结果表明，使用这种方法能够精确地得到人脸图象各局部特征的位置；⑤提出了一种新的人脸图象表示法---双属性图表示法；利用属性图来表示人脸图象，并提取图节点对应图象位置的局部主成分特征系数以及Gabor变换系数作为图节点的属性，这种表示方法充分利用了人脸图象的局部特征的统计特性，并且使用Gabor变换来反映人脸图象的拓扑结构，从而使得双属性图表示法具有较强的通用性；⑥在双属性图表示的基础上，给出双属性图匹配函数及匹配算法，在函数及算法设计过程中，考虑并解决了光照、尺度、旋转姿势变化等因素对人脸图象识别的影响，使得匹配算法具有较强的通用性；⑦设计并实现了一个通用的人脸图象识别系统，实验结果表明，该系统在图象光照、尺度、旋转姿势情况下，得到了较好的识别效果。

His shocking illiterate ideas, attempting to correct "learning from others" to "learning from oneself","being educated" to "educating oneself","to destroy is to construct" to "to construct is to elutriate","aesthetic judgment, ugliness judgment, and others judgment" to "self judgment, thought judgment, importance judgment and thingliness judgment","make nothing out of something" to "to make something out of nothing"(we have to create something, because there is nothing), and to instinctively inspire one's intelligence and to make "empty reproduction" and "analysissitus" of such an inspiration, encouraged more and more pioneers to inaugurate new things.

把"学习他人"纠正为"学习自己"，把"他人教育"纠正为"自我教育"，把"破坏就是建设"纠正为"建设就是淘汰"，把"审美和审丑及审他"纠正为"审我审想审要审物"，把"有中生无"纠正为"无中生有"（正因为没有，所以才要创造），以及对智慧的本能激发和对本能的智慧激发的"空殖"、"悬浮"、"拓扑"等惊世骇俗的文盲观点，激发了越来越多的先锋创新人士。

Through the Radon transformation of the normally ordered Wigner operator we introduce two mutually conjugate intermediate coordinate- momentum representations. Based on them we construct the appropriate quantum phase space theory which includes the new Wigner operator adapting to this space and construct the appropriate generalized Fredholm operator equation and then find its solution. We then deriving the Hermite polynomials operator identities by applying the Fredholm equation. We also reveal the connection between the generalized Wigner operator and the 2-dimension normal distribution in statistics, which is useful to study the quantum tomogram. As the application of the entanglement Husimi operator theory we calculate the Wigner function and the Husimi function of the one- and two-mode combination squeezed state , study their characters through drawing the three-dimensional graphics.

由正规序Wigner算符的拉登变换引入了两个互为共轭的中介坐标-动量表象，在此基础上我们建立了相应的量子相空间理论，其中包括引入适合该空间的新的Wigner算符；并在该表象的基础上，建立了广义Fredholm算符方程，求出了它的解，并运用该方程导出有关厄米多项式的算符公式；揭示广义Wigner算符与统计学中的随机变量的二维正态分布形式上的相似，这对于研究量子态的tomogram(是英文Tomography的派生词)有用。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。