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Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

The paper analyses material transportation problem and give the solvable methods; analyses delivery vehicle route problem, and then construct its corresponding mathematical model and present a heuristic algorithm based on C—W algorithm; analyses the optimum scheduling problem of trucks, and then construct its corresponding mathematical model and put forward a new algorithm; analyses the Postal Transportation Problem which will perhaps be used in supply chain of manufacturing system, and then construct its corresponding mathematical model and present an approximation algorithm.

分析了制造系统供应链中供需平衡、供需不平衡、有转运等各种情况下的物资调运问题，给出了求解方法；分析了制造系统供应链配送作业中的配送车辆路线问题，建立了相应的数学模型，提出了一种基于C-W算法的启发式算法，该算法较好地解决了有时间约束的配送问题；分析了制造系统供应链中的货运卡车优化调度问题，建立了相应的数学模型，提出了一种基于表上作业法的求解算法；分析了制造系统供应链中有潜在利用价值的邮政运输方式问题，在详细描述了该问题的基础上，建立了相应的数学模型，提出了一种近似求解算法，该算法较好地解决了邮政运输方式这样的多种物流、多种运输方式，有严格时限和众多外部约束的特大型、复杂、并行的交连系统的计划调度问题。

Constructing _bstr_t bs1 ="char string";// construct from a LPCSTR _bstr_t bs2 = L"wide char string";// construct from a LPCWSTR _bstr_t bs3 = bs1;// copy from another _bstr_t _variant_t v ="Bob"; _bstr_t bs4 = v;// construct from a _variant_t that has a string // Extracting data LPCSTR psz1 = bs1;// automatically converts to MBCS string LPCSTR psz2 = bs1;// cast OK, same as previous line LPCWSTR pwsz1 = bs1;// returns the internal Unicode string LPCWSTR pwsz2 = bs1;// cast OK, same as previous line BSTR bstr = bs1.copy;// copies bs1, returns it as a BSTR //...

一个_bstr_t字符串能够传给一个接收参数类型为BSTR的函数，只是因为下列3个条件同时满足。首先，_bstr_t有一个向wchar_t*转换的转换函数；其次，对编译器而言，因为BSTR的定义，wchar_t*和BSTR有同样的含义；第三，_bstr_t内部含有的wchar_t*指向一片按BSTR的形式存储数据的内存。

China municipal road informational construct just begins, and the municipal ITS comprehensive solving plan doesn't start yet. China Police Security has gradually regarded municipal road traffic informational construct as the emphases of city informational construct, to apply to the development of city economic and the economic globalizes' require.

目前我国城市道路交通信息化建设才刚刚开始，实施城市 ITS 全面解决方案高技术产业化工程尚未启动，公安部已逐步把城市道路交通信息化建设作为城市信息化建设的重点，以适应城市经济的发展和经济全球化的需要。

Constructing _bstr_t bs1 ="char string";// construct from a LPCSTR _bstr_t bs2 = L"wide char string";// construct from a LPCWSTR _bstr_t bs3 = bs1;// copy from another _bstr_t _variant_t v ="Bob"; _bstr_t bs4 = v;// construct from a _variant_t that has a string // Extracting data LPCSTR psz1 = bs1;// automatically converts to MBCS string LPCSTR psz2 = bs1;// cast OK, same as previous line LPCWSTR pwsz1 = bs1;// returns the internal Unicode string LPCWSTR pwsz2 = bs1;// cast OK, same as previous line BSTR bstr = bs1.copy;// copies bs1, returns it as a BSTR //...

这是一个值得怀疑的设计，因为即使它们是非常量字符串指针，你也一定不能使用这些指针去修改它们指向的缓冲区的内容，因为那将破坏内部的BSTR结构。

The project's aim is to construct an industrial base whose size is 100thousand mous;to construct one production line of the deep processed goods includesQinJiantea(600t/year),instant tea (10millionbox/year) QinJiam tea Buccal Tablet (10millionbox/year),etc.to construct one refinery production line of flavonol(output is 100t/year)and one refinery production line of Dihydromyricetin (outputis 50t/year).

总投资10000万元，用三至五年时间，完成&秦简茶&产业基地建设10万亩；建成秦简茶(600吨/年)，速溶茶(1000万盒/年)，秦简茶含片(1000万盒/年)等深加工系列产品生产线及黄酮素(100吨/年)，二氢杨梅素(50吨/年)提取生产线各一条。

On the side of global, the measures which are answerable for the food crisis are: first to construct the safeguard mechanisms of planting area, secondly to construct the anti-disaster farming system, thirdly to construct the information share platform on crop reservoir and demand, and finally to carry out Sci-tech supporting project on food security.

从全球层面看，应对当前粮食危机的主要措施是：一是构建全球层面上的粮食安全生产的耕地保障机制；二是在全球视野中构筑灾害应对型农作制度；三是构建全球粮食贮备及需求信息共享平台，稳定粮食供应市场；四是构建全球粮食安全科技支撑计划，提升粮食生产科技水平。

The basic idea is to make party's internal operation mechanism healthy with integration of the from above to bottom supervision and that from bottom to above; to construct the wholly-coursed supervision and restriction mechanism with integration of the supervision before, in and behind the matter; to construct all-sided supervision and restriction mechanism with integration of party's internal and external supervision, and to construct the supervision and restriction mechanism of comprehensive governance with integration of self-restriction and outer-restriction, rule of law and rule of morality.

基本思路为：把自上而下的监督和自下而上的监督有机结合，健全党内监督的运行机制；把事前、事中、事后监督有机结合，建立全程化的监督制约机制；把党内监督和党外监督有机结合，建立全方位的监督制约机制；把自律和他律、法治和德治有机结合，建立综合治理的监督制约机制。

In order to construct a normalized cDNA silencing library, a novel procedure was adopted to reduce the abundant copy number and increase the rare copy number. Then the normalized cDNA was put into pTRV vector to construct a cDNA library and this cDNA library was screened with the technology of virus induced gene silencing.

然后把均一化之后mRNA反转录得到cDNA，再与基因沉默载体pTRV重组，最后把构建好的载体通过电转化的方法转入到GV3101农杆菌中，从而建立起破色期番茄果实均一化cDNA沉默文库。

However, the strategy and rhetoric that these Puritans employed to construct their American identity were replicas of those employed by the Anglicans in their effort to construct an identity for the English as God's chosen people in a fight against the Antichristian influence of the Roman Catholic Church.

然而清教徒移民建构认同的策略，实系挪用自十六、十七世纪时英国新教徒建构自我与英国国家认同的策略，只不过不同於移民美洲的清教徒，英国新教徒自视为上帝的选民，反基督的他者则是罗马天主教。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？