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The ubiquitin proteolytic system is an important selective proteolytic system of the celler proteolytic systems, a number of them were identified as a ubiquitin chain assembly factor-E4. The U-box protein is a new family ubiquitin-protein ligase(E3), which determine the substrate specificity of ubiquitination. The U-box is a domain of 70 amino acids that is highly conserved among yeast, plant and animals, but more U-box proteins were found in plants than in animals.

泛素系统是选择性降解细胞内蛋白质的重要系统之一，U-box蛋白质是此系统中决定底物特异性识别的一种新型E3蛋白质，部分U-box蛋白质属于泛素链聚集因子-E4.U-box结构域大约由70个氨基酸残基构成，在酵母、植物和动物等真核生物中保守存在，但植物中的数目远多于动物中。

Activity of β-galactosidase in sigL mu-tant strain was much lower than it in wild-type strain and sequence analysis showed that the domain of AcoR and BkdR are similar to the conserved domain of SigL-dependent transcriptional activator in Bt.

序列比对分析表明：Bt中的AcoR和BkdR的蛋白结构域与依赖于sL的转录调节因子的保守序列相似。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Molecular cloning and characterization of the novel lection gene. Primers were designed based on conserved sequences of other lectin genes from Amaryllidaceae. A novel lectin gene was isolated from Zephyrathes grandiflora. The full-length cDNA of Zephyrathes grandiflora lectin was 955bp, containing an open reading frame (576bp) encoding a peptide of 191 amino acids. The gene was named as zga (Zephyrathes grandiflora agglutinin), with its encoding protein showing 59% similarity to GNA.

基因克隆及序列分析：根据石蒜科植物凝集素的保守区段，设计引物，采用RACE技术从韭莲中克隆出长度为952bp的新凝集素基因，包含了完整的编码序列(576bp)，它编码一个全长191个氨基酸的蛋白，与雪花莲外源凝集的相似性达59％，被命名为ZGA(Zephyrathes grandiflora agglutinin)。

Our results indicate that the stigmas appear to have conserved and novel molecular functions between rice and Arabidopsis.

我们的结果提示了水稻和拟南芥柱头具有保守功能和新功能。

The minichromosome maintenance proteins are extensive existenced and conserved throughout archae to eukaryotic cells.

微小染色体维持蛋白（minichromosome maintenance proteins，MCMs）是一类从太古代生物到高等真核生物广泛存在的高度保守的蛋白。

We provide evidence that general O-glycosylation systems are conserved among intestinal Bacteroides species and likely contribute to the predominance of Bacteroides in the human intestine.

我们提供证据表明，完整O-糖基化系统在肠道的拟杆菌属种中具有保守性，并可能有助于确立拟杆菌属在人肠道的数量优势。

Five pairs of 45S rDNA loci and two pairs of 5S rDNA signals were detected on chromosomes of Cucurbita moschata Duch. Luffa cylindrical Roem. contained five pairs of 45S rDNA loci and one pair of 5S rDNA loci. In Benincasa hispida Cogn., two pairs of 45S rDNA sites and one pair of 5S rDNA site were detected. In this species, 5S rDNA and one pair of the 45S loci were collocated closely in chromosome 7S. 45S rDNA chromosomal distribution patterns were highly conserved among the three species, althoufh their number varied markedly. The 5S rDNA sites on chromosomes among the three species were highly polymorphic.

南瓜有5对45S rDNA位点， 2对5S rDNA位点；丝瓜具有5对45S rDNA位点， 1对5S rDNA位点；冬瓜具有2对45S rDNA位点， 1对5S rDNA位点， 5S rDNA位点与其中一对45S rDNA位点都位于7号染色体短臂上，并在物理位置上紧密相邻。45S rDNA在这3种作物染色体上数目变化较大，但在染色体上都倾向分布在短臂末端，其分布模式较为一致。5S rDNA在这3种作物染色体上数目相对保守，但在染色体上分布的位置变化较大。

Of the 36 tussah silkworm varieties, the Nei's genetic diversity index was 0.3243±0.1515, and Shannon information index was 0.4871±0.1975, suggesting that bivoltine tussah silkworm varieties are still rich in genetic diversity even having been conserved in the same place for quite long time. Nei's genetic identity of the 36 varieties ranged from 0.4959 to 0.8347, 96.59% of which was above 0.6, revealing large similarity between different varieties. A clustering analysis showed that these varieties formed two separate clades, one of which included varieties bred by the institute and the other included imported varieties from other provinces or countries.

供试品种资源的Nei氏遗传多样性指数为0.3243±0.1515，Shannon信息指数为0.4871±0.1975，表明长期同地保存的二化性柞蚕品种资源同样具有丰富的遗传多样性；供试品种的Nei氏遗传一致性范围在0.4959~0.8374之间，达0.6以上的占96.59%，表明多数品种资源的相似程度较高。36份品种资源分别以辽宁省蚕业科学研究所育成种为主和以国内外引进种为主聚为两大类群。

The results demonstrated that MC1R gene of bird was relatively conserved. The results of phylogenetic analysis showed that consanguinity relations between mule duck, anas platyrhynchos and branta bernicla, anser caerulescens, anser canagica were close.The status of them were confirmed on zoologyl by means of molecular method.

可见禽类的MC1R 基因编码区序列具有高度的保守性；系统进化分析表明，半番鸭、北京鸭与雪雁、黑雁、皇雁亲缘关系相近，从分子角度验证了它们在动物学分类上的地位。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。