查询词典 conjugated

2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.

根据dystrophin基因5个多态位点（44、45、49和50号内含子以及5'端启动子区的序列）设计引物，在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾：CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身，经高分辨率的变性聚丙烯酰胺凝胶电泳后，利用红外激光遗传分析系统及SAGA〓基因分型软件对凝胶上的扩增产物及内标的位置，进行扫描分析，确定产物片段的大小。

The recombinant protein Pep10 conjugated with GAMP showed strong carrier effect, which provided a novel route for the development of ideal carrier protein.

制备的重组蛋白Pep10与A群脑膜炎球菌多糖偶联能发挥较强的载体效应，为研究理想载体蛋白提供了新的思路。

One can imagine the eventual emergence of a general purpose computer consisting of nothing more than a single macromolecule conjugated to a ribosomelike collection of enzymes which act upon it.

我们可以想像届时将会出现一种泛用电脑，这种电脑只由单一巨分子和各种可作用于巨分子的酵素集合体所制成。

In order to detect the distribution of silica-binding proteins in rice and other graminaceous plants, specific polyclonal antibodies against a silica-binding protein from rice, namely SBP117, are successfully raised by synthesized peptides Which are conjugated with Keyhole Limpet Hemocyanin and used as antigens to immunize rabbits.

为了检测硅结合蛋白在水稻和禾本科植物体内的分布，根据硅结合蛋白(silica-binding protein 117， SBP117)的氨基酸保守片段和天然抗原表位，合成了SBP117的两个肽段做抗原，与匙血蓝蛋白载体偶联后免疫兔子，获得了抗SBP117的专一性抗体。

The hapten was conjugated to horseradish peroxidase to prepare enzyme tagged hapten by means of mixed anhydride method.

在此基础上，首次成功建立了对三唑磷具高特异性的间接竞争、包被抗体直接竞争酶联免疫吸附分析技术。

Methods Prepare conjugated antigen BSA-Aβ by coupling hapten Aβ1-42 to vector protein BSA with glutaraldehyde method.

用戊二醛法将半抗原Aβ1-42偶联于载体蛋白BSA，形成结合抗原BSA-Aβ。

The synthesized hapten of triazophos was covalently conjugated to bovine serum albumin and ovalbumin respectively to prepare artificial antigen and coating antigen.

将合成的三唑磷半抗原采用活性酯法分别与牛血清白蛋白和卵清蛋白共价偶联制备突出三唑磷分子结构特征的人工抗原与包被抗原。

Methods PIWIL4 peptide was synthesized using chemical method, and conjugated to Keyhole limpet hemocyanin as immunogen.

方法合成特异性PIWIIA多肽，以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原，通过致敏大白兔，制备兔抗人PIWIIA多克隆抗体，然后用亲和层析法纯化抗体。

Specific polyclonal antibodies against SBP117 are successfully raised by synthesized peptides which were conjugated with Keyhole Limpet Hemocyanin and used as antigens to immunize rabbits.

人工合成SBP117的两个肽段与匙孔嘁血蓝蛋白载体偶联后免疫兔子，获得了抗SBP117的专一性抗体。

A serial of novel thiophene-based main chain conjugated compound with varieties of aromatic rings and heterocycle, such as acenaphtenequinoxaline phenanthrenequinoxaline ,have been synthesized on the base of looking for lots of references and summarized others research work.

在查阅大量文献和综合前人所作工作的基础上，我们选择卤代芳烃与Sn金属有机试剂在Pd催化下合成了以萘基喹喔啉和菲基喹喔啉为主的一系列噻吩与杂环构成的交叉共轭化合物。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。