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Methods Conjugated with protein precipitation with trichloroacetic acid, BCA method was used. According to the positive relationship of protein content with the 570 nm absorbance, protein content was calculated in powdered milk.

使用三氯乙酸沉淀蛋白质后，运用BCA法，在570nm波长处，分别测定标准蛋白质应用液与样品稀释液的吸光度值，基于测定液中蛋白质含量与其吸光度值呈正比关系，计算出样品中蛋白质的含量。

With an increase in Bcl-2 protein, more Bax homodimers were separated and conjugated with Bcl-2 protein to form more stable Bcl-2/Bax heterodimers than Bax homodimers, which'neutralize'apoptosis induction of Bax protein.

随着Bcl-2蛋白增多，越来越多的Bax蛋白同源二聚体被分开，与Bcl-2蛋白结合形成比Bax-Bax蛋白更稳定的Bcl-2-Bax蛋白杂二聚体，从而&中和&Bax蛋白的诱导细胞调亡作用。

Our preliminary study showed that the ratio of carbohydrate conjugated to the protein carrier is great increased when compared to commercial available carbohydrate carrier. We then apply it in animal study. After immunizing the BALB/c mice with this carbohydrate immunogen, the induced antibodies against carbohydrate were examined by western blotting and dot blotting.

实验的结果，我们设计的蛋白质载体，确实能让醣类抗原高效结合，且在西方点墨法及圆点点墨法的实验中显示，用此抗原所免疫的老鼠血清，可辨认所结合的醣类抗原。

Methods: penicillin, as a kind of hapten, was conjugated with carrier protein to form complete antigen and then was used to immunize balb/c mice. hybridoma cells secrecting mab against penicillin were developed.

将半抗原青霉素和载体蛋白偶联后免疫balb/c小鼠，应用杂交瘤技术建立稳定分泌抗青霉素mab的杂交瘤细胞株。

Thus, the "yellow enzyme" is isolated as a conjugated protein in which the non-amino acid moiety is riboflavin phosphate, while catalase is an iron-porphyrin-containing protein

比如说，分离出来的结合蛋白-&黄酶&便是一例，它的非氨基酸部分是磷酸核黄素。而过氧化氢酶则是一种含卟啉铁的蛋白。

The expressed E2 protein was conjugated to ELISA microplate which was precoated with anti-E2 mAb H53. Then Raji cells were seeded onto the microplate and cultured .

将表达的E2蛋白固定在事先包被了E2单抗的酶标微孔板上，然后加入Burkitt淋巴瘤Raji细胞，可观察到该细胞聚集贴附生长。

The items of investigation on physical and mental health included general information, rational symptom, function of autonomic nerve and sensory nerve, blood routine, conjugated bilirubin , serum protein , glutathione-s-transferase, malondialdehyde, reproductive functions, state of mental health.

工人身心健康调查项目包括一般情况、自觉症状、自主神经和感觉神经功能、血常规、血中结合胆红素、血清蛋白、谷胱苷肽-S-转移酶和丙二醛、女工生殖功能和心理健康状况。

The results implied that NO/ROS-induced ABA accumulation come from de novo synthesis pathway rather than degration of conjugated forms. The protein synthesis inhibitor actidone blocked the NO-induced ABA accumulation while having no effects on ROS-induced ABA accumulation, indicating that the action of NO was gene-expression dependent and that of ROS was gene-expression independent. These results suggested that NO may serve as a stress signal in the regulation of ABA-biosynthesis and ROS may serve as substrate in ABA biosynthesis or be involved in the regulation of enzyme activities.

1ABA合成抑制剂fluridone抑制ROS和NO诱导的ABA积累，表明NO／ROS诱导的ABA积累来源于ABA的从新合成而不是已有结合态ABA的代谢；(2)蛋白质合成抑制剂环己亚胺可以抑制NO诱导的ABA积累而对ROS诱导的ABA积累没有影响，说明NO的作用涉及基因表达而ROS的作用与基因表达无关，暗示NO的作用方式可能是作为一种干旱胁迫信号物质参与ABA合成的调节，而ROS则可能参与了相关酶活性的调节或直接参与了ABA合成的底物反应。

A specific peptide according to FMDV NSPs B-cell epitopes was synthesized by a solid-phase method, and was conjugated with carrier protein BSA. An ELISA for detecting FMDV NSPs antibody was developed by using the conjugating protein as the coating antigen.

固相法合成特异性FMDV NSP B细胞表位肽，将其偶联在BSA载体蛋白上，作为抗原包被在ELISA板上，制备检测抗FMDV NSP抗体的ELISA试剂盒，并对该试剂盒进行方法考核。

The Pncps-protein conjugants prepared were helpful to promote the transfer of the classes of antibody to Pncps from IgM to IgG in serum. Under our experimental conditions, when the usage of Pncps was identical in the conjugants, the more protein conjugated, the more IgG titers to Pncps and to protein were observed.

本实验制备的荚膜多糖－蛋白的结合物中，相同多糖用量情况下，蛋白用量的增加能够促进血清中抗多糖抗体类型由IgM向IgG转变，分别提高结合物抗多糖、抗蛋白IgG抗体滴度。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。