查询词典 conjugate

The isomerization principle of independent double bonds of unsaturated fatty acids from Chinese featural arboroid oils-stillingia oil was first studied in the paper in China. The synthesis principle, properties of products and practical experiment of the derivatives of conjugate acids were also discussed.

本文以中国特产木本油脂—梓油为原料，在国内首次对油脂不饱和脂肪酸孤立双键的异构化反应规律进行了系统的研究，并以异构化反应所得共轭酸为原料，对共轭酸衍生物系统产品的合成规律、产品性能及应用性实验进行了一定程度的探索。

The optimum pH for labeling was between 7.5-8.0, and the optimum spiking rate of polyclonal antibody was 12μg/ml. The absorb pad, sample pad, glass fiber of ARIST and nitrocellulose membrane HF24004 of Millipore were chosen as GICA materials. The carbofuran-OVA conjugate 0.97mg/ml and sheep anti-rabbit IgG 0.48 mg/ml was dispensed with Nano-Plotter (NP 1.2) onto the NC membrane to serve as test line and control line, respectively. The colloidal gold-labeled anti-carbofuran antibody was dispensed on the glass fiber with 20μl/unit.

选用ARIST公司的吸水垫、样品垫、玻璃纤维纸和Millipore公司的HF24004硝酸纤维素膜作为试纸条材料，用三维喷点平台分别将0.97mg/ml的克百威-OVA结合物和0.48 mg/ml羊抗兔IgG间隔5mm平行定量喷涂固化于硝酸纤维素膜上作为测试线和质控线，将金标记抗克百威多克隆抗体以20μl/垫定量滴加于玻璃纤维纸上，干燥硝酸纤维素膜和玻璃纤维纸。

METHODS:AsOR was conjugated to PL by EDC; The active ester of PEG derivative was reacted with the amino groups in PEI to form PEG-PEI conjugate; DNA was first complexed with PEG-PEI and then with AsOR-PL. In vitro transfection experiment was conducted in the galactose receptor positive Huh-7 cells.

分别制备AsOR-PL偶合物及PEG-PEI偶合物，然后按照不同的比例先后与报告基因DNA形成复合物，再进行Huh-7细胞的体外转染试验和静脉给药后小鼠体内的表达试验。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

The third-order optical nonlinearities and lime responses were measured by a phase conjugate forward, 3-dimensional degenerate, four-wave mixing technique. Comparing polymer's third-order NLO coefficient, it shows that the χsuperscript (3 value increasecs with the introduction of the azobenzol side-chain.

通过简并四波混频飞秒激光系统对各个接枝聚合物的三阶非线性极化率系数进行了测定，考察了偶氮侧链的引入对聚合物主链三阶非线性的影响，并考察了侧链上不同取代基的电子效应对主链三阶非线性的影响。

It is shown that the first hyperpolarizability β of barbituric acid deviratives strongly depends on the donor structure and the length of conjugate chain, but is not sensitive to the acceptor structure. The HOMO-LUMO calculations reveal that the first hyperpolarizabilities of barbituric acid deviratives are linear to the HOMO-LUMO energy gap △E.

研究结果表明，给体结构与共轭链长度对第一超极化率β影响较大，而受体结构对分子第一超极化率的影响很小，并且分子第一超极化率与分子前线轨道HOMO与LUMO的能级差△E呈较好的线性关系。

Grasp the electron delocolization theory; be capable of judging the relative stability of allylic and benzylic free radicals and carbocations, know the relative stability of dienes and other multiple unsaturated hydrocarbons; grasp the characteristics for the nucleophilic substitution reactions of allyl halides and benzyl halides, and the addition reactions of conjugate dienes.

掌握电子非定域化理论；能够分析判断烯丙基、苄基自由基和正离子的稳定性和二烯及多重不饱和碳氢化合物的稳定性；掌握烯丙基、苄基卤代物的亲核取代反应特性和共轭二烯的加成反应。

In the fifth chapter, a series of betulin derivatives including 28-acetoxybetulin, 28-tritylbetulin, 28-benzoylbetulin, 3-0-P-D-glucopyranoside of betulin and some betulinic acid conjugate containing amino acid were synthesized and confirmed by MS and NMR.

第五章分别合成得到了乙酰化白桦酯醇、28-苯甲酰化白桦酯醇、28-三苯甲基化白桦酯醇、3-O-β-D-glucopyranoside of betulin和一系列的白桦酯酸苷氨酸的衍生物，并分别对其进行了结构鉴定。

In the fifth chapter, a series of betulin derivatives including 28-acetoxybetulin, 28-tritylbetulin, 28-benzoylbetulin, 3-O-β-D-glucopyranoside of betulin and some betulinic acid conjugate containing amino acid were synthesized and confirmed by MS and NMR.

第五章分别合成得到了乙酰化白桦酯醇、28-苯甲酰化白桦酯醇、28-三苯甲基化白桦酯醇、3-O-β-D-glucopyranoside of betulin和一系列的白桦酯酸苷氨酸的衍生物，并分别对其进行了结构鉴定。

Objective To synthesize 10-O-adipic acid-[2'-methoxy-4'-(3"-keto-1"-Butenyl)-Phenoxy-]-dihydroartemisinin conjugate.

目的 合成10-O-己二酸基－［2'-甲氧基4'-（3&-酮－1&-丁烯基）－苯酯］－双氢青蒿素酯。

In the days of sailing vessels the crew were afraid they would be becalmed on the ocean.

在使用帆船的时代，船员们担心他们会因为无风而无法在海洋上航行。

As long as foreign donors pay the PA's salary bill, few expect a new intifada.

只要外国继续为巴权力机构的薪水买单，希望发动新暴动的人便寥寥无几。