查询词典 conical flask

In this experiment, 250mL Erlenmeyer flask with 50mL reaction liquid(400mmol/L glucose, 10mmol/L CMP, 50mmol/L choline phosphate, 100mmol/L K 2HPO 4 KH 2PO 4, 10mmol/L MgSO 4, pH=8.0) and 1 200g/L immobilized cells were used. After 4h incubation 400mmol/L of glucose was supplemented.

结果表明：2 5 0 m L摇瓶中加入 5 0 m L反应液(葡萄糖 40 0 mmol/ L，CMP1 0 mmol/ L，磷酸胆碱 5 0 mmol/ L，K2 HPO4 -KH2 PO4 缓冲液 1 0 0 mmol/ L，Mg SO4 1 0 mmol/ L，p H=8.0 )，固定化细胞 1 2 0 0 g/ L，3 4°C、1 5 0 r/ min下，反应 4h后补加 40 0 mmol/ L葡萄糖，反应 8h可使60 %以上的 CMP转化为 CDP-胆碱。

The optimum culture medium components of DA07405 contained glucose 1.0%, sucrose 0.5%, corn powder 0.5%, soybean powder 0.75%, K2HPO4 0.05%; The optimum fermentation conditions of DA07405 were initial pH7.0, seed age 48h, 12% inoculation amount, the size 75mL in 250mL Erlenmeyer flask at 200 r/min and 28℃ for 5d.

确定菌株DA07408的最佳培养基配方及发酵条件为：葡萄糖1.0%，甘油1.5%，玉米粉0.75%，大豆粉0.75%，K2HPO4 0.05%，初始pH值7.0，种龄48h，接种量10%，200r/min发酵5d，培养温度28℃，250mL三角瓶装液量75mL。

With the single factor test and orthogonal test, the optimal culture media for HF3-04 strain producing agarase was decided as: agar 3‰, NH4NO31‰, Na2HPO4 0.5 mM, pH 8.0. The optimal fermentation conditions were inoculum size 3‰, 110ml medium in 250mL Erlenmeyer flask, rotation rate 150r∕min, culture time 48h,culture temperature 25℃.

通过正交试验确定其最佳培养基组成为琼胶3‰、硝酸铵1‰、磷酸氢二钠0.5mM、pH值8.0；该菌株的最佳培养条件为接种量1%、装液量为250ml三角瓶装入110ml发酵培养基、摇床转速150r∕min、培养温度25℃、培养时间48h。

He paid $5,000 for this cupcake.

他我这个纸杯蛋糕花了5000美元。

I mean, if you want to, that is

我的意思是，如果你们想吃的话。