查询词典 confirmed

On the basis of which, the grade of coarse aggregates is confirmed. The experimental results are validated through the theory calculation.(2)The reasonable ratio of coarse aggregates to fine aggregates is confirmed by the mathematics mold method and interrupted aggregate gradating experiment.

并以此为基础，确定了粗集料的级配，使振实后的粗集料能够达到最大的密实度，同时应用理论计算对试验结果进行验证通过振实试验和容重方法研究了不同粒径粗集料混合后的空隙率。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Consider that processes the factor such as being convenient, the whole frame adopts the stencil plate to press the shaping craft, so as to ensure the whole intensity of the frame; The main body of the frame has continued to use the traditional ladder-shaped structure, of simple structure, stability is good; Assign the scheme wholly according to the electric automobile, has confirmed the physical dimension of the frame; Combine the scheme of decorating of the main spare part of electric automobile, has confirmed the frame is released, the crossbeam one decorates a form and connects the method, install the needing of main spare parts such as the gear box,etc. in because some crossbeams, has taken the structural form of the ingot roof beam.

考虑到加工方便等因素，整个车架采用钢板冲压成型工艺，以保证车架的整体强度；车架主体沿用了传统的梯形结构，结构简单，稳定性好；根据电动汽车整体布置方案，确定了车架的结构尺寸；结合电动汽车主要零部件的布置方案，确定了车架纵、横梁的布置形式及连接方法，部分横梁因为安装变速器等主要零部件的需要，采取了元宝梁的结构形式。

Reveal the characteristics and regular pattern of its epidemic.①Investigated the epidemiological history of 448 clinical confirmed SARS cases, drawing the map of coming places of primary cases and whereabouts of the secondary cases, clarify the reasons of SARS inputting and spreading.②Exploring transmission relationship between cases by drawing the chain-map and learning the main transmission process.③Selecting the chain-cases with the exact onset and contact time to calculating the average incubation of same generation cases on different transmission chains and the average incubation of different generation cases on same transmission chain, Calculating the incubation median, the fifth percentile and the ninety-fifth percentile of the incubation of the observed cases.④Analyzing the timeliness of admission and the treating condition through the statistical calculation of the onset time, the admission time, the hospitalization time as well as the diagnostic time of the suspect and clinical confirmed cases.

调查全省448例SARS临床确诊病例的流行病学史，绘制全省输入SARS原代病例的主要来源以及引起传播后被感染者的去向图，阐明SARS病例的输入与扩散原因；②用传播链图分析传播关系，了解SARS的主要传播过程；③选择SARS原代病例与续发病例接触日期、发病日期明确而完整的传播链病例计算不同传播链同一代之间以及同一传播链不同代之间的平均潜伏期，并计算观察病例潜伏期的中位数以及第5、第95百分位数，确定SARS的最短潜伏期和最长潜伏期；④对患者发病、就诊、住院时间以及医院诊断为SARS可疑病例、临床确诊病例的时间进行统计，分析SARS就诊的及时性以及临床诊治情况；⑤计算不同接触方式、关系密切程度传播SARS的危险度，筛选形成传播的主要危险因素；⑥检测不同人群和同一人群不同时间的SARS血清抗体，了解抗体的阳转时间和持续时间。

Results: In the early phase of the diseases, both showed abnormal X-ray manifestations with normal or reduced white blood cells. But mucosal changes in pharynxes were different in confirmed and suspicious patients. All suspicious SARS patients had an obvious pharyngeal inflammation including a lot lymphoid follicles over soft palates, and showed a good response to the treatment with antibiotics and antiviral drugs in one week. In contrast, seven confirmed SARS did not have an obvious pharyngitis.The clinical symptoms did not been improved ,but had been deteriorated at day 10 after the onset after antibiotic and antiviral treatment.

结果：临床诊断及疑诊SARS病例临床初期均有白细胞下降或正常，以及胸部χ线异常，但两者咽部黏膜表现有所不同，1 3例疑似病例均有明显的咽部炎症，淋巴滤泡密集覆盖软腭，经抗菌、抗病毒治疗后1周内好转，7例临床诊断SARS病例咽部炎症不明显，经抗菌、抗病毒治疗后临床症状未见明显好转，且多在病程1 0 d后加重。

If the enterprise installation IS for the core competence, the interrelated element include culture, organization and distribution management and the aim of the management paradigm has been confirmed, and the"authority"has been confirmed too, then the resistance and motivity are a frame that to consider the sequence in the management changing based on IS.

若企业为了实现某核心竞争力而实施企业信息系统，相关要素包括文化、组织和销售管理，且已经确定了各管理模式的变革目标（在管理模式连续分布场中的位置）、相对核心竞争力的&权&重，那么&权&、关于变革的阻力和动力的力场分析和各管理模式之间的关系就是考虑基于信息系统的管理变革的一个完整框架，可以先按&权&重排队，然后考虑制约力和驱动力。

Buyers to apply for subsidies, with "Shitai County residents to purchase goods Checklist for housing subsidies," confirmed their household registration information, ID cards, purchase contracts, purchase invoices and registration certificate to the county real estate property Real Estate Management Construction Committee Bureau report, confirmed after verification, the county real estate Construction Committee Governance Council within 15 working days after the payment of subsidy payments to subsidize the object directly punch.

购房人申请补贴时，凭《石台县农村居民购买商品住房补贴申请审核表》广州发票自己户籍证实资料、身份证、购房合同、购房发票和房地产产权登记证到该县建委房地产治理局申报，经确认核实后，由县建委房地产治理局在 15 个工作日内将补贴款直接打卡发放给补贴对象。

Payments for the goods under the present contract are to be effected in USD dollars in an irrevocable confirmed letter of credit, which is opened for the consignment of the Goods by the Buyer in Novosibirsk branch of NOMOS BANK and confirmed in JP MORGAN CHASE BANK, N.Y., USA.

此合同的将使用不可撤销的信用证来支付美金。此信用证是由买家通过NOMOS银行新西伯利亚支行开给出货人，并由美国纽约的MORGAN CHASE银行确认此信用证。

That we wish this so to be observed, appears from the fact that of our own free will, before the outbreak of the present dispute between us and our barons, we granted and confirmed by charter the freedom of the Church's elections - a right reckoned to be of the greatest necessity and importance to it - and caused this to be confirmed by Pope Innocent III.

我们希望这一明显由我们自己的自由意志决定的条款将得到遵守，毋论现在我们和我们的男爵们之间的纠纷，由本宪章确认教会选举自由－－这项权利对教会而言是至关重要的－－并经教皇Innocent III确认。

FIRST, THAT WE HAVE GRANTED TO GOD, and by this present charterhave confirmed for us and our heirs in perpetuity, that the EnglishChurch shall be free, and shall have its rights undiminished, and itsliberties unimpaired. That we wish this so to be observed, appears fromthe fact that of our own free will, before the outbreak of the presentdispute between us and our barons, we granted and confirmed by charterthe freedom of the Church's elections - a right reckoned to be of thegreatest necessity and importance to it - and caused this to beconfirmed by Pope Innocent III.

首先，我们用这份摆在面前的为了我们自己及后嗣而共同确定的宪章永久的应许上帝：英格兰的教会应当自由，应当拥有它的权利和自由，不得侵害；我们希望这一点被注意到，在这次我们和我们的巴仁们的争议爆发之前，用我们自己的自由意愿，我们用宪章应许和确定了教会选举的自由这个事实——一项被认为是对于自由意愿而言最必要和重要的的权利——曾由教皇英诺森三世确认。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。