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The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

The optimal conditions for electroporation transformation of Pichia Pastoris were obtained with the maximum transformation efficiency of 36 transformants/μg plasmid DNA when the intermediate logarithmic phase yeast cells were used for competent cells, and the electroporations were under the conditions of voltage of 1.5kV and 0.15mg / mL plasmid DNA and 0.2 cm cuvettes.

结果表明：当采用对数生长中期的菌体制备感受态细胞、电压为 1.5kV、质粒浓度为 0.15μg/μL和 0.2 cm 电转化杯时，转化效率达到最大值，为每微克质粒 DNA 36个转化子。

Nanjing Green State machine factory Decanter centrifuge design engineer Yan Chun Min, qualitative analysis of the sediment conditions Decanter centrifuge drum cone angle with a knife, screw conveyor blade angle B, blade pitch, liquid pool radius and the difference between speed An elaborated to improve the conditions of slag lose Decanters design ideas.

南京绿州机器厂卧螺离心机设计所高级工程师颜春敏，定性地分析了卧螺离心机的沉渣条件与其转鼓的锥角刀、螺旋输送器叶片的倾角B、叶片的螺距、液池半径及差转速An之间的关系，阐述了改善卧螺离心机输渣条件的设计思想。

The identification of crucial periods and conditions for the development of diabetes-related cognitive decrements helps to draw the attention of physicians to individuals at risk and can direct future studies into the mechanisms that underlie these conditions.

发生糖尿病性认知下降的主要时期和条件的确定可以帮助医生注意那些有风险的患者并能指导将来对其发生机制的研究。

On the basis of wide investigation and research on oil derricks, combined with the practical use of oil derricks on the spot, a comprehensive statement is made in this paper about the basic conditions, the structural features and its practical uses of oil derricks on the spot, a comprehensive statement is made in this paper about the basic conditions, the structural features of oil derricks and its practical use.

本文的主要内容可以归纳为：(1)、本文在对石油井架进行广泛调研的基础上，结合石油井架在现场使用的实际，对石油井架的基本情况、石油井架的结构特点以及石油井架在油田生产中使用的实际进行了综合的论述。提出了有关石油井架承载能力评定的理论和实际的方法。

According to the algorithm analysis on defintion of the dewpoint in the gas contaning LPG, the dewpoint expression under different conditions can be deduced and a safe content limit for C5 constituent in the gas under different conditions can also be determined.

通过对含有LPG的燃气的露点计算方法的分析，导出在不同条件下的露点表达式。

In China, right of classified into two kinds: statutory right of revocation and consensual right of revocation. I analyze conditions of applying them. There are three conditions of fulfilling statutory right of revocation, which aims to punish the donees'ungrateful behaviors.

同时介绍了我国撤销权分为法定撤销和任意撤销，分析了法定撤销权和任意撤销权的行使条件，法定撤销权行使条件有三个，其立法目的是为了惩罚受赠人的&忘恩负义&行为。

Channels earthworks due to geological conditions, soil conditions, such as the impact of environmental factors is rather complicated.

渠道土方工程因地质条件、土质情况、环境因素等影响而比较复杂。

The following exciting results are revealed: 1 When considering the influence of some nonlinear elements such as hard-limit of exciters, the effects of some devices such as Power System Stabilizer and Static Var Compensator which can introduce positive damping in power system, the reasonability of system parameter values in simulation, the SNB surface and HB surface on the boundary of SSSR will turn close to each other and even coalesce together; 2 Under some conditions, coupling between slow exciters and shunt capacitors will bring negative damping in power system dynamics, which increases the possibility of oscillatory instability. So the power system with mass shunt compensators is easily subjected to the oscillatory instability. In recent years, chaotic phenomena of power system have been reported many times. Some simulation studies even found chaos existing inside the power system SSSR. In this dissertation, chaotic phenomena in power systems are thoroughly studied in order to make clear the relation of chaos and SSSR. The following results are derived: 1 Based on Li-Yorke Theorem and their definition on the chaos, the existence of chaos in power system is verified; 2 Three possible routes of causing chaos in power system are found and deeply investigated. They are route of cascading period doubling, route of directly initial energizing and route of torus bifurcation (or quasi-periodicity). The latter two routes are investigated for the first time in power system stability studies; 3 When the stability conditions of chaos are broken, it is found they can lead to voltage collapse, angle divergence, or voltage collapse with angle divergence simultaneously.

针对在电力系统小扰动稳定区域内可能存在混沌吸引域的有关报道，本文深入研究了电力系统混沌现象的出现途径和与系统失稳模式之间的关系：1利用Li-Yorke定理和Li-Yorke的混沌定义，从理论上证明了电力系统混沌现象的存在性；2发现了电力系统中导致混沌出现的三种可能途径：倍周期分岔导致混沌、初始能量直接激发混沌和经由环面分岔导致混沌，并对后两种新发现的途径进行了较为细致的研究；3发现了混沌极限环破裂导致电压崩溃、角度失稳以及电压崩溃和角度失稳同时出现的现象，其中混沌极限环破裂导致系统角度失稳和电压崩溃及角度失稳同时出现的现象均属首次报道；4证明由微分-代数方程描述的系统模型，其小扰动稳定域的边界只包含HB、SNB和SIB三种分岔界面，在SSSR的内部和边界上，均不可能存在会导致混沌的点，从而将混沌现象排除在小扰动稳定域的研究之外，简化了后者的研究工作。

Traditional exhaust silencer can not match to the variable working conditions, thus it has difficulty to reduce noise substantially in all working conditions.

传统有源消声器的消声特性，无法随发动机工况变化，因而难以在全工况范围内取得良好的消声效果。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。