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Background and Objective The goals of this study were to label the antisense oligodeoxynucleotides indirectly by Chloramine T method and Iodogen method, then to discuss the labeling yields、radiochemical purity、specific activity and stability in different conditions. We discussed the right labeling method and conditions in order to lay the experiment foundation of the antisense medicament labeled by iodine for clinical application.

目的与研究背景:本课题采用氯胺-T法和Iodogen法两种标记方法进行反义寡脱氧核苷酸的125I间接标记对比研究,并在两种方法中分别探讨不同反应条件下的标记率及标记产物的放化纯、比活度和稳定性。

First, a chlorinator was self-designed in this paper, and dichloride of 2-methyl-1-propene was synthesized by 2-methyl-1-propene reacted with chlorine fast in this reactor. By means of the Orthogonal Experiments design and single factor experiments, the influence of several reaction variables on the yield were examined and the optimum reaction conditions under the intervallic operation were obtained, the yield and selectivity of dichloride of 2-methyl-1-prop-ene were more than 65% and 80% respectively. Second, the preparation of 2-methylene-1, 3-propanediol, which dichloride of 2-methyl-1-propene reacted with alkali was investigated. By means of the Orthogonal Experiments design and single factor experiments, the influence of several reaction variables on the yield were examined and the optimum reaction conditions under the intervallic operation were obtained, the conversion of dichloride of 2-methyl-1-propene was 100%. Moreover the preparation of MPO, which 2-methylene-1, 3-propanediol reacted with hydrogen, in presence of Al_2O_3-Pd catalyst, was gotten, and the product was appraised.

本文在氯化反应过程中,自行设计制作出了氯化管式反应器,在该反应器中异丁烯和氯气迅速混合反应生成二氯异丁烯,根据该氯化反应特征利用正交试验和单因素试验考察了有关因素的影响并优化得到了最佳工艺条件,二氯异丁烯的总收率达65%以上,选择性达80%;接着,对二氯异丁烯进行水解反应制得了2-甲烯-1,3-丙二醇,讨论了相关因素对水解反应的影响,并通过正交试验和单因素试验得到了较佳工艺条件,二氯异丁烯的转化率达100%;同时还对制备的2-甲烯-1,3-丙二醇在常压下采用三氧化二铝负载钯催化剂催化加氢得目的产物MPO,并对产品进行了鉴定,从而打通了整个工艺路线。

Confirmation of high-pressure pelitic rocks in the north. The mineral assemblage of the early stage is gamet-Mg-rich chloritoid +phegite +quartz ±kyanite ±aragonite or gamet+Mg-rich chloritoid+phengite+paragonite+quartz, P-T conditions are 550±30℃ and 1.4~2.0GPa; the mineral assemblage of the late stage is muscovite +chlorite+quartz±albite, which undergone retrograde metamorphism of greenschist facies, P-T conditions are <550℃ and <0.6GPa.

确定北部高压变泥质岩石,早期矿物组合为石榴子石+富镁硬绿泥石+多硅白云母+石英±蓝晶石±文石或石榴子石+富镁硬绿泥石+多硅白云母+钠云母+石英,对应的温压条件为550±30℃和1.4~2.0GPa;晚期矿物组合为白云母+绿泥石+石英±钠长石,经受了绿片岩相的退变质作用,其温度小于550℃,压力低于0.6GPa。

The optimal conditions of the pepsin hydrolysis of frame protein in catfish Claris lareza were investigated using a general-purpose rotation unitized design of the second power of four factors, and the content of soluble peptides was determined in the hydrolytic products. The Optimization of Design Expert 7.1.2 revealed that antibacterial activity of the frame protein hydrolysates was controlled by regulation of four process conditions.

采用四因子二次通用旋转设计,以抑菌率为测定指标,对胃蛋白酶水解革胡子鲶Claris lareza鱼骨蛋白的条件进行了优化,同时测定了酶解产物中可溶性肽的含量,以分析抑菌率与可溶性肽含量之间的相关关系。

At present, with the development of the science and technology, wind conditions can be deduced from various satellite climograph, this paper will adopt wave spectral numerical models to offer wave conditions with enough resolution and accuracy, taking into account that errors may occur during directly evaluating wave parameters from empirical formulas.

现今科技的发展使人们能从卫星气象图片计算出风场,为避免由经验公式从风场推算波高分布的误差,本文将利用波浪谱数学模型以提供具有足够分辨率和精确度的波浪场。

Hydraulic conditions and water balance conditions Generally heating, air-conditioned closed loop pipe network are all pipe network, and its hydraulic system condition is the pressure point, the pipe flow, pressure.

二、水力工况和水力工况平衡一般地说供热、空调的管网都是闭路循环的管网,其水力工况是指系统各点的压力,各管段的流量、压差。

A contract with collateral conditions on its entry into effect shall become effective upon the fulfillment of the conditions.

附生效条件的合同,自条件成就时生效。附解除条件的合同,自条件成就时失效。

Conditions for the super-fine comminution were studied by orthogonal experimental design and as a result the significant effect was observed in 3 different experimental conditions.

综述了小麦麸的组成、食疗作用以及综合利用的概况;并采用正交法,就小麦麸的超微细化加工进行了实验,结果表明介质因素有显著影响。

By applying the comparison theorem and some new analysis technique, some sufficient conditions which guarantee the permanence of the system are obtained. After that, by constructing a suitable Lyapunov function, sufficient conditions which ensure the global attractivity of the system are obtained.

利用差分方程的有关理论,使用与前面不同的分析方法,获得保证该系统永久持续生存的充分性条件;其后通过构造适当的差分Lyapunov函数,得到保证该系统全局吸引的充分性条件。

The four major Dao bodies are complementary in function, and their relationship is that associations operate on the basis of the information and logistic systems, information and logistic systems provide the functional conditions for the natural operation of associations, and the awakening culture provides the intelligent conditions for the natural operation of associations.

四大道体具有功能互补的性质,相互之间的关系是:协会组织在信流系统和物流系统的基础上运行,信流系统和物流系统为协会组织提供自然运行的功能条件,觉醒文化为协会组织提供自然运行的智能条件。

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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

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