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The results show that the optimal hydrolysis conditions of these three enzymes were:50℃,pH 8.5, substrate concentration 1:3,enzyme dosage 1200U/g,time 4h for Alcalase; 45℃,pH 8.1, substrate concentration 1:3, enzyme dosage 1200U/g, time 4h for Trypsin;50℃, pH5.5, substrate concentration 1:3, enzyme dosage 1400U/g, time 6h for Bromelin, respectively. The compound enzymes hydrolysis was investigated on the basis of the single enzyme hydrolysis.

利用单因素试验和正交试验分别对这三种酶单独水解牡蛎的条件进行了研究，得出三种酶水解牡蛎的最佳条件分别为Alcalase：温度50℃，pH8.5，料水比1：3，加酶量1200U/g，时间4h；胰蛋白酶：温度45℃，pH8.1，料水比1：3，加酶量1200U/g，时间4h；菠萝蛋白酶：温度50℃，pH5.5，料水比1：3，加酶量1400U/g，时间6h；为牡蛎生物活性肽的开发提供了基础参考数据。

The results showed that temperature rise would inhibit their esterification capacity, ethyl caproate-producing capability of raw zymin of 1# high-temperature monascus improved with the prolong of the esterification time when ethanol concentration was 4 %vol and caproic acid content was 4 mL, and when ethanol concentration and caproic acid content was within proper range, caproic acid content had more effects on the formation of ethyl caproate by raw zymin than ethanol concentration.

结果表明，温度相对升高对其酯化力的形成有一定的抑制作用；高温型红曲1#酯化菌株粗酶制剂的酯化力在乙醇浓度为4 %vol、己酸含量为4 mL时，产生己酸乙酯的能力随酯化时间的延长而增强；在适宜的乙醇浓度和己酸含量范围内，己酸含量对酯化酶粗酶制剂己酸乙酯生成影响比乙醇含量的影响大。

The results show that during bioleaching the chalcocite decom posed by two stages, the stageⅠis controlled by the concentration of ferric, with the increase of ferric concentration the oxidation rate of chalcocite is enhanced. The rate of stageⅡis slower than that of the stageⅠand it acts as the confining stage, and the concentration ratio of ferric to ferrous influences the oxidation rate at this stage.

计算与实验结果表明：模型仿真结果与实际结果较符合；堆浸过程次生硫化铜矿的氧化分为2个阶段，其中，辉铜矿反应步骤Ⅰ受溶液Fe3+浓度控制，随着Fe3+浓度增加，反应速率加快；反应步骤Ⅱ较反应步骤Ⅰ慢，为浸出反应的限制性步骤， Fe3+与Fe2+的浓度比是影响该反应步骤的关键因素。

The indigenous microorganisms existed in the contaminated soil could degrade low concentration of chlorophenol 00mgg^(-1, however, their degradative capacity was limited when chlorophenol concentration was high about 500mgg^(-1, which may be caused by the toxic effect of high concentration of chlorophenol on the indigenous bacteria.

土壤中存在的土著的氯酚降解菌可以对低含量的氯酚100mgg^(-1进行降解；但是当土壤中氯酚含量较高500mgg^(-1时，土著微生物的降解能力受到限制，这可能是高浓度的氯酚对土著的氯酚降解菌会产生毒性作用。

Result : 1. E xperimental study:(1)After 48 hours culture,the morphology of K562 cells that FFJZ's concentration is 1mg/ml and less than 1mg/ml became fusiform and claviform.The K562 cells that FFJZ's concentration is 2mg/ml has no conspicuous different.The K562 cells that FFJZ's concentration is 4mg/ml and 6mg/ml took on shapes of crenation.

结果：1、体外实验：（1）K562细胞经FFJZ作用48小时后，浓度在 1mg/ml及以下组出现细胞形态呈梭状、棒状，伴有胞浆丝状突起形成伪足，2mg/mlFFJZ组细胞与对照组无明显差别，4mg/ml及6mg/mlFFJZ组，细胞出现皱缩、破碎； 8mg/mlFFJZ及10mg/mlFFJZ组细胞经培养24小时后，即出现细胞明显变小，且均匀分散，以后时间里均呈现此种状况。

Comparing the electrolyte concentration shifting along the time with the membrane surface potential shifting along the electrolyte concentration, the crimple phenomenon is related to the shift rate of electrolyte concentration and to the shift range of the membrane surface potential.

通过模型计算环境中平均盐浓度随时间的变化及实验测量膜表面电位随盐浓度的改变，我们发现皱褶的出现及形变程度与盐浓度变化的急剧程度有关，也与膜表面电势变化幅度有关。

There were no significant correlations between food concentration and the rate of population increase of Clone B as well as the mixis rate and fertilization rate of Clone A, B and C. However, the relationships between food concentration and the rate of population increase of Clone A and C were significantly curvilinear, with the regression equation Y=0.019X2-0.209X+0.638 and Y=0.042X2-0.402X+0.852, respectively. The relationships between the food concentration and the instant rate of population increase, the mixis rate and the fertilization rate of clone D were all significantly curvilinear, with the following regression equations Y=-0.052X2+0.609X-0.667,Y=0.994X2-15.763X+73.424 and Y=-0.251X2+15.940X-15.446, respectively.

克隆B的种群瞬时增长率、种群中的混交雌体百分率和混交雌体受精率均与食物密度间无显著的相关性；克隆A和C的种群瞬时增长率与食物密度间呈曲线相关，而其种群中的混交雌体百分率和混交雌体受精率均与食物密度间无显著的相关性；克隆D的种群瞬时增长率、种群中的混交雌体百分率和混交雌体受精率均与食物密度间呈曲线相关。

Glyphosate brought the biggest vice-effects, clethodim took the second and fenoxaprop-p-ethyake minimum:(1)As to the pure growth, MDA accumulation, the relative permeability of plasma membrane and chlorophyll content, glyphosate had evident influences on them in the daily dosage, 1/2 and 1/4 of the daily dosage . The walnut seedling dried and died twenty days later.(2)The influences of clethodim in different concentration such as double daily dosage, daily dosage and 1/2 of daily dosage on the above four indexes demonstrated differently, the more concentration was, the more severe the influence. was. With the concentration reducing, the influence weakened gradually.(3) Double daily dosage, daily dosage and 1/2 daily dosage of fenoxaprop-p-ethy exerted a slight vice-influence to the MDA accumulation, the relative permeability of plasma membrane and chlorophyll content.

1用草甘膦常用量、1/2倍常用量、1/4倍常用量处理之后，对核桃苗新梢生长量、叶片中丙二醛含量、膜相对透性以及叶片中叶绿素含量的影响最大，几乎所有样枝的新梢在药后20天后都发生干枯及死亡现象；(2)用收乐通2倍常用量、常用量、1/2倍常用量处理之后，对核桃苗的新梢生长量、叶片中丙二醛含量、膜相对透性及叶片中叶绿素含量的影响，呈现出浓度不同影响亦不同的现象，浓度越大，影响越明显，随着浓度的降低，影响也逐渐减弱；(3)用威霸2倍常用量、常用量、1/2倍常用量处理之后，对核桃苗新梢生长量、叶片中丙二醛含量、膜相对透性及叶片中叶绿素含量的影响最小；(4)三种除草剂对核桃苗的影响随着药后时间的延长，其影响在逐渐减小。

Therefore, in order to study pharmacology of astragali, atractylodes macrocephala and licorice root active component infurther step, search for reasonable extraction technology of effective parts, develop safe and reliable,exact curative effect new drug for treating UC fit to clinic, we plan to use advanced colon-target andadhesion releasing technology to make up complex prescription colon-target capsule preparation ofabove-mentioned three effective parts. This will have important significance for developing new drugstudy on treating UC with TCM.Study method1. pharmaceutical research1.1 study on extraction and abstraction technology of Astragalus mongholicus saponinAdopt ethanol backstreaming to extract Astragalus mongholicus saponin, compare extraction percentage of different concentration ethanol. Adopt resin adsorptive process to depurate it, inspect resin dynamic adsorption curve, sample size, concentration and dosage of eluting ethanol and concentration of eluting lye and so on technology parameter. Determine the best extraction and abstraction technology.

目前国内外未见有治疗该病的中药有效部位的复方制剂，因此，为进一步研究黄芪、白术和甘草有效成分的药理学作用，探索合理的有效部位提取工艺，开发适合于临床的安全可靠，疗效确切的治疗UC的中药新药，我们拟采用先进的结肠靶向定位及黏附释药技术，将黄芪、白术和甘草的上述三类有效部位制成复方结肠溶胶囊制剂，对于开展中医药治疗溃疡性结肠炎的新药研究具有重要意义。

A nonlinear regression is adopted to analyze the changes of plasma vitellogenin concentration in crucian carp via the environmental pollutant exposure concentration, and the concentration-response relationship is determined for the single chemical of estradiol (E2), ethynylestradiol (EE2), bisphenol A, 4-tert-octylphenol, and that of the mixed compounds at equipotent concentrations, the mixture being tested using a fixed-ratio design.

通过对鲫鱼血浆卵黄蛋白原含量的相对变化和暴露浓度的非线性回归分析得出17α-乙炔基雌二醇(EE2)、17β-雌二醇(E2)、双酚A、辛基苯酚及其等毒性固定浓度比例混合物产生雌激素效应的剂量－反应关系；应用相加作用数学模型根据单个化合物的数据预测混合物效应。

Liapunov—Schmidt method is one of the most important method in the bifurcation theory.

Liapunov—Schmidt方法是分叉理论的最重要方法之一。

Be courteous -- even when people are most discourteous to you .

要有礼貌──即使当別人对你最不礼貌的时候。