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The alkalescent condition reduces the mass concentration of iron ions and fastens the nitration reaction of ammonia nitrogen, and leads to the concentration of nitrate.

偏碱性条件使铁离子质量浓度降低，同时促进了氨氮硝化反应进行，使矿井水中硝酸盐和亚硝酸盐质量浓度增加。

In the complex medium, it was found that adding alanine and aminoacetic acid could obviously improve the production of PNP ase and there was interaction between them. When the alanine concentration was in 80~140 mgL^(-1) and aminoacetic acid concentration was in 140~200 mgL^(-1), the high yield of fermentation could be ensured.

在复合培养基中，丙氨酸和甘氨酸对产酶有明显促进作用且存在交互作用，两者浓度分别控制在80~140 mgL^(-1)和140~200 mgL^(-1)之间时能够很好地保证发酵产量。

The glutamine synthetase produced by the engineering bacteria can specially act on NH4 and aminoglutaric acid, the durability to the high concentration ammonium ion has been distinctively improved because of the dissolution of adenosine acylation modification, the major bottleneck of glutamine with enzymatical production has been solved, and the concentration of the obtained glutamine can reach 47.6g/L.

本发明工程菌产的谷氨酰胺合成酶可专一性的作用于NH 4 和谷氨酸，且因解除了腺苷酰化修饰作用对高浓度铵离子的耐受能力显著提高，解决了酶法生产谷氨酰胺的重要瓶颈，生成的谷氨酰胺的浓度最高达到47.6g/L。

In this research, the spider silk from the major ampullate gland of native Nephila pilipes was collected via the forced-silking apparatus at the reeling speed of 3 m/min. The obtained silk was dissolved in hexafluoro-isopropanol. The influence of pH and temperature over the secondary structure of the silk protein in solution state was examined via Circular Dichroism. The effect of silk concentration and electrical field strength on the structure conformation of silk protein during film casting process was investigated via FT-IR. During the drying process, the increase of silk concentration and the presence of electric field tend to form β-sheet and β-turn. Finally, the dried film of silk protein was treated with different temperatures and cation solutions to study the post-treatment effects.

本研究所采用之蜘蛛丝蛋白质系取自本土品种人面蜘蛛之大囊壶腺体，以3 m/min之卷丝速度强迫取丝(forced-silking)收集蜘蛛丝，再将纤维溶於六氟异丙醇溶剂中配制成溶液，以圆二色光谱仪分析在不同 pH 值与温度条件下丝蛋白质在溶液中之二级结构变化，再将蜘蛛丝溶液以不同浓度及电场强度下乾燥成薄膜后，以傅立叶红外线光谱仪检测分析丝蛋白质在固体状态下之二级结构，最后以不同温度加温、离子溶液浸泡等条件对乾燥所得之薄膜进行后处理，并分析其二级结构之变化。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

The results indicated that light control could induce Mongolian sheep to estrus and ovulate in anoestrum; there was a significant effect on concentration of MLT in blood serum of Mongolian sheep, but there were no significant effects on concentration of FSH and LH in blood serum of Mongolian sheep.

结果表明：光照控制可诱导蒙古羊在非繁殖季节发情并排卵，对蒙古羊血液MLT分泌影响显著，而对血液FSH和LH分泌没有显著影响。

The effects of doping concentration of iodine, doping time, doping temperature and exposure time on volume resistivity of anthracite were studied; and also the effects of solution concentration of cupric chloride, doping time, doping temperature, soking time of cupric chloride and exposure time on the volume resistivity were studied; Anthracitic resistivity has been decreased in dissimilar extent, and furthermore ,volume resistivity stability of the doping anthracite made by liquid phase doping method is more better than gas phase mothed; Resistivity and its stability were better than the doping method of iodine in fused salt doping method of cupric chloride.

研究了I_2掺杂浓度、掺杂时间、掺杂温度和暴空时间对无烟煤电阻率的影响；探讨了CuCl_2溶液的浓度、掺杂时间、掺杂温度和CuCl_2溶液浸泡放置时间以及暴空时间对无烟煤电阻率的影响：结果发现：I_2气相掺杂和液相掺杂均使无烟煤的电阻率有不同程度的降低，而液相掺杂法所得材料的电阻率稳定性更高；CuCl_2熔盐掺杂法所得材料的电阻率及其稳定性均优于I_2掺杂法。

The activities of antioxidase became higher with increasing Cu concentration, and had a positive correlation with Cu concentration.

Cu胁迫下金星蕨抗氧化酶活性随Cu浓度增加而上升，且与Cu浓度呈极显著正相关。

There were no concentrations less than 5 mcg/mL; Average concentration of all pre-hemodialysis was 12.6 ± 3.6 mcg/mL. Average concentration of all pre-hemodialysis for anuric patients (n=10) was 14.2±3.6 mcg/mL.

结果：17位病患，总共获得102个药物浓度，全部透析前浓度介於5~20有49 (96.1%)个浓度，大於20有2 (3.9%)个浓度，最低浓度为7.22，最高为22.75，没有低於5的浓度；全部血液透析前浓度的平均值为12.6±3.6；没有残余肾功能的患者(n=10)平均的血中浓度为14.2±3.6 mcg/mL。

However, when the concentration of nitrate was 21 mg/L, nitrite with the concentration of 106 mg/L has an approximative potential.

因而，利用亚硝态氮，尤其是硝态氮低浓度条件下，强化反硝化条件下河流沉积层中苯降解是可行的。

Liapunov—Schmidt method is one of the most important method in the bifurcation theory.

Liapunov—Schmidt方法是分叉理论的最重要方法之一。

Be courteous -- even when people are most discourteous to you .

要有礼貌──即使当別人对你最不礼貌的时候。