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The biometallogenesis system of Jinya gold deposits consists of the following procedure: pre-concentration of gold and metallogenic matter in living organisms in Luodian-Danchi rift depositional basin; solution, absorption and concentration of metallogenic matter in organic matter and organic thermal fluid generated from organisms during lithogenesis; migration of gold and metallogenic matter in organic fluid from basin to Lingyun underwater carbonate platform and concentration at its boundary convex (palaeo-blind-mountain) during basin compaction ; metallization due to depth thermal fluid making organic fluid thermal degradation and forming new fluid which riches in gold and sulfur, and the fluid migrating upward and depositing in upside ore-controlling structures because of absorptions by organic carbon and atc.

通过研究金牙金矿作用的生物成矿系统内容为：在早、中三叠世罗甸—丹池裂谷盆地接受沉积时，生物对金等成矿物质就存在预富集作用；在成岩过程中，生物转化为有机质及有机热流体，对生物及沉积物中的金等成矿物质存在溶解、萃取、富集作用；在盆地压实成岩作用中，盆地中有机热流体向凌云水下碳酸盐台地运移，并在台地边缘外凸部位集中，此过程流体携带大量成矿物质，对金等成矿物质存在迁移集中作用；深部热流上侵使有机流体发生降解和释放金的热作用、形成高含金的有机热液，含金热液上移在构造通道中沉积并被有机碳再次吸附，最终形成金矿床，对金存在成矿作用。

The inhibition rate were -5.15%,-13.29%, 29.16%, 63.19% and 61.89% when the drug concentration levels were 0.389, 0.648, 1.08, 1.8 and 3mg/mL, respectively. The half inhibitory concentration of Marsdenia Tenacissima extract A was 1.8 mg/mL using regression method. The maximum of no toxicity concentration of Marsdenia Tenacissima extract B towards HepG2 was also approximately 0.648mg/mL.

结果：乌骨藤提取物A对HepG2细胞的最大无毒浓度大约为0.648mg/mL；药物浓度在0.389、0.648、1.08、1.8、3mg/mL时的抑制率分别为-5.15%、-13.29%、29.16%、63.19%、61.89%；用回归法求出乌骨藤提取物A对HepG2细胞增殖的半数抑制浓度为1.8mg/mL。

This method conformed the following conditions: spleen phosphodiesterase and micrococcal nulcease for digesting reagent and the concentration, temperature and digesting time of the two enzymes;the components of p32-post label alleviant and labeling time;the concentration of p1 nuclease; the concentration of T4 polynucleotide kinase for catalysing and labeling reagent;the components of bidirectional spreader and the spreading time ,etc.

确定了采用脾磷酸二酯酶和微球菌核酸酶消化底物以及两种酶的使用浓度、温度及消化时间；确定了P32标记缓冲液的主要成分及标记时间；确定了P1核酸酶的浓度；确定了T4多核苷酸激酶催化标记底物的浓度；确定了双向展开剂的主要成分及展开时间等。

Results showed that urokinase at 20U/ml was the optimal concentration used. Fibrinolysis kinetics and plasma plasminogen concentration of 27 normal persons and 27 patients with acute myeloblastic leukemia were detected, a significant difference was observed between the two groups. Repetition test showed that the coefficience of variation was 5.24±1.51%. This method could also be used to detect the concentration of plasma fibrinogen.

结果 初步建立了正常人纤溶动力学分析参数，确定了20U/ml尿激酶为最适试验浓度，检测22例正常人，27例急性髓细胞白血病患者纤溶动力学及纤溶酶原含量，显示患病组较正常组差异有显著意义(P.05， P.01)，重复性试验显示该方法的变异系数为(5.42±1.51)%，并可同步测定血浆纤维蛋白原浓度。

After NMDA stimulation, he concentrations of Ca~2+ and NO in both nuclear and plasma rise observably, especially in the netlike structures; the change of Ca~2+ concentration in nuclear is larger than that in plasma,however the changes of NO concentration in nuclear and plasma are similar. We also observed comparative increases of the concentration of Ca2+ and NO in neurodendrite after NMDA stimulation.

NMDA刺激后神经元细胞核和细胞质内Ca~(2+)和NO都明显升高，尤其在细胞核和细胞质内的网状结构处；细胞核内Ca~(2+)升高幅度大于细胞质，而细胞核内NO的升高幅度却与细胞质相近；另外，树突内的Ca~(2+)和NO升高也较明显。

By orthogonal experimental design method, the optimized degumming parameters have been confirmed: preacid processing, the concentration of sulfuric acid 2 g/L, treatment time 120 min, treatment temperature 60℃, bath ratio 1:15; alkali treatment, the alkaline concentration of one-pot 7.5 g/L, that of two-pot 9 g/L, treatment time of one-pot 3.5h, that of two-pot 2h, treatment temperature 100℃, bath ratio 1:20, each concentration of the Sodium Silicate, carbamide, and JFC 2%.

预酸处理，硫酸溶液质量浓度2 g/L，处理时间120 min，处理温度60℃，浴比1：15；碱处理，一煮碱溶液质量浓度7.5 g/L，二煮碱溶液质量浓度9 g/L，一煮处理时间3.5 h，二煮处理时间2 h，处理温度100℃，浴比1：20，其中一煮与二煮中添加2%的硅酸钠、尿素和JFC。

The results showed that: the explants of strawberry leaves were rather sensitive to kanamycin, and the corresponding concentration of 20 mg/L could substantially inhibit the growth of non-transformed tisse and be used to screen the leaves of strawberry after transformation. Timentin at the concentration of 300 mg/L could be used to eliminate Agrobacterium tumefaciens and had minor impact on the growth of the explants. The preculture time of 2-4 days was beneficial to improve transformation frequency. Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium. The bacterial concentration of OD(subscript 600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

结果表明：草毒叶片外植体对卡那霉素较为敏感，适宜的筛选浓度为20mg/L，可明显抑制非转化组织的生长；300mg/L的羧吩青霉素可有效抑菌，对外植体生长影响也较小；预培养2-4d有利于转化；共培养2-3d有利于提高转化频率并避免了农杆菌的过度生长；OD(下标 600nm)值为0.4的菌液侵染10min效果最佳。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Relative contents of four main cellular proteins corresponding to MALDI-TOF-MS peaks m/z 4466, m/z 6455, m/z 6514, and m/z 7772 were all found to be close correlated with the concentration of melamine, although their up- and/or down-regulations showed different patterns when the concentration of melamine changed. Support vector regression leave-one-out method, with the optimized polynomial kernel function and a poly-degree of 3, was chose to evaluate the correlation between the alteration of Tetrahymena pyriformis main proteins'relative contents and the concentration of melamine in the culture medium.

支持向量机软件中支持向量回归support vector regressi(来源：A3cBC17论b1文网www.abclunwen.comon， SVR留一法预报结果表明，选用幂指数为3的多项式核函数进行建模，相对误差的平均值为7.238%，说明梨形四膜虫主要蛋白质相对含量的变化与培养基中三聚氰胺浓度变化相关。

The nursery water was the best, when microencapsulated diets of "OK" and rotifera were blended at the percentage of 80% and 20%, the nursery water quality was the best during the rearing period, the highest concentration of NH3-Nt (NH3-Nm) was 1.025mg/L (0.0836mg/L),and the highest concentration of NO2-- N was 0.0236mg/L. When larvae emerged, the concentration of COD was 1.526mg/L, Puyrrop Hta was 1000 Entries/ml, and the metamorphic rates of all periods exceeded symmetrically 97%, and the survival rates also higher than the control group fed pure crude diets. The emergence status in P7 period was described as follows: the metamorphic rate: 73.33%; the average tail weight: 2mg; average body length: about 7.604mm; which were 18.30%, 19.3%, 8.4% higher than that of control group respectively.

其中80％欧开牌微胶囊饲料和20％卤虫配合使用的育苗效果最好，在整个育苗期间该实验组水质较好，NH_3-N_t(NH_3-Nm)含量最高为1.025mg／L(0.0836mg／L)，NO_2~--N_2含量最高为0.0236mg／L，出苗时COD为1.526mg／L，甲藻类含量为1000个／ml，幼体各期的变态率较稳定均衡，均大于97％，成活率也高于只投喂天然饵料的对照组，至仔虾第7天(P_7)出苗时，出苗率为73.33％，平均尾重为2mg，平均体长达7.604mm，分别比天然饵料的对照组高出18.30％、19.3％和8.4％，各项出苗指标均明显高于对照组(P＜0.05)；且虾苗活泼健壮、体表洁净、均匀度高。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。