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The Zoea Ⅳ had LC50 of 0.085 and 0.047 mg/L at 24 h and 48 h, and safe concentration of 0.0043 mg/L, indicating that the LC50 and safe concentration increased with the development of the larvae. The megalopa had LC50 of 0.085 and 0.050 mg/L at 24 h and 48 h, and safe concentration of 0.0052 mg/L.

分别为0.019 mg/L和0.010 mg/L,安全质量浓度为0.0038 mg/L;三疣梭子蟹Ⅱ期溞状幼体24 h LC50和48 h LC50分别为0.085mg/L和0.047 mg/L,安全质量浓度为0.0043 mg/L;三疣梭子蟹大眼幼体24 h LC50和48 h LC50分别为0.085 mg/L和0.050mg/L,安全质量浓度为0.0052 mg/L。

Their 95% effective concentration (EC95) value was 0.4735 μg/mL. Flumorph at the concentration of 0.5 μg/mL, can lead the inhibit rate of growth of mycelium, formulation of sporangium and germination of zoospore of Phytophthora sojae to 100%, the concentration was lower than Apron XL, Metalaxy which lead to the same inhibition rate. It indicated that Flumorph can obviously inhibit the growth of P.sojae in vitro, and effect is better than that of Apron XL and Metalaxy.

结果表明:氟吗啉对所测定的菌株4个发育阶段均有显著的抑制作用,氟吗啉对大豆疫霉菌菌丝生长EC50 值为0.1906μg/mL,EC95 值为0.4735 μg/mL;在0.5 μg/mL 浓度下对大豆疫霉菌孢子囊形成和孢子萌发抑制率达100%,低于达到相同抑制效果的金阿普隆和甲霜灵;说明该药剂在离体条件下能够有效抑制大豆疫霉菌的生长,效果好于金阿普隆和甲霜灵。

The ultrafilteration is applied in the separation and concentration of the zymin, it can be operated in the normal temperatures, overcome the effects heat does to the quality of the fermented products, abstract small molecular admixtures, and guarantees filtrates purity and quality; Filtering process does not change, the energy consumption and the cost compared with the traditional method are lower; The equipment running is simple, and is easy to operate and the control, thus it quickly became concentration separation craft the first choice of concentration separation such as biology medicine manufacture.

现在采用超滤技术进行酶制剂的分离浓缩,则可在常温下操作,克服热对发酵产品品质的影响;除去其中的小分子杂质,保证滤液的纯净度和品质的良好性;过滤过程无相变,能耗和成本较传统方法都低;设备运行简便,易于操作和控制,因而很快成为了生物制药等行业分离浓缩工艺的首选。

The experimental results showed that the partition coefficients and yields of 2,3-butanediol and acetoin reached 11.9,9.10,83.48%, 79.37%,respectively,when the concentration multiple was 70%,the concentration of K_2HPO_4 added was 45%,and the concentration of 2,3-butanediol in top phase reached

结果表明:当发酵液浓缩70%后,加入45%的磷酸氢二钾,乙偶姻、2,3-丁二醇的分配系数最高,分别为11.9、9.10;回收率较高,分别为83.48%、79.37%;上相中2,3-丁二醇浓度最高可达420g/L。

The optimal determining conditions were obtained: adding quantity of a monolayer medium 15ml, the suspension concentration of Micrococcus luteus 109 cfu/ml, the adding quantity of Oxford Plate 100μl, agar concentration 1%, the concentration of Na2HPO4 1%, and the pH of the medium adjusted to 7.0. The medium was optimized by single factor and the orthogonal experiments on the basis of the centrifugate of distiller's grains. The optimal composition was obtained as follows: sucrose 0.6, peptone 0.1, yeast extract 0.2, K2HPO4?

以管碟法为基础,对影响Nisin效价测定的各种主要因素进行了分析,并得出了Nisin效价测定的最佳条件:采用单层培养基(每个平板加量为15ml),检测培养基中Na2HPO4·12H2O浓度改为1%,菌悬液浓度109cfu/ml,不加吐温20,琼脂浓度1%,培养基pH7.0,牛津杯加液量100μl ,30℃培养24小时。

After the oxidative polymerization of DMP,styrene was in-situ polymerized under the initiation of dibenzoyl peroxide and dicumyl peroxide,finally thermodynamically compatible PPO/PS alloy was prepared.When styrene content was 50 wt%,for the synthesized PPO/PS alloy the yield and the weight-average molecular weight were determined to be 95%and 1.7×10~5 for PPO,93%and 2.0×10~5 for PS,respectively.The influence of the concentration of DMP and catalyst,molar ratio of N/Cu, oxygen partial pressure,NaOH concentration and temperature on the initial polymerization rate(R_0) of DMP was studied.It was found that the initial polymerization rate of DMP increased with the concentration of DMP and catalyst. The initial polymerization rate increased with the molar ratio of N/Cu at first and then decreased.

通过测定DMP氧化聚合过程中氧气吸收量随时间的变化,研究了DMP和催化剂浓度、N/Cu摩尔比、氧气分压、氢氧化钠浓度及温度对水介质中DMP氧化聚合初始速率的影响,发现DMP氧化聚合初始速率与氧气分压的0.1次方成正比;DMP氧化聚合初始速率随DMP和催化剂浓度及温度的增加而增加;随氢氧化钠浓度和N/Cu摩尔比的增加DMP氧化聚合初始速率先增加后降低。

It improved the quality of grapy fruits: a very small amount of nanoparticles increased the concentration of selenium for about 5 times, the concentration of sugar increased by about 30%, and that of calcium increased by about 40%, but the concentration of acid decreased by 30%, compared with contrast teams.

淀粉-硒纳米颗粒作为植物营养调节剂应用于巨峰葡萄植株,成熟葡萄果实中的硒含量比对照提高了5倍,糖份提高了30%,钙含量提高了40%,而酸含量则降低了30%,大大改善了葡萄的风味。

To our knowledge, reverse phase of high performance liquid chromatography was firstly developed to analyze the metabolites of 7-tocopherol with ideal results. Meanwhile, Gas chromatography/Mass chromatography were used to analyze the structures of unknown metabolites of 7-tocopherol and a main unknown metabolite was identified as a side chain ω- hydroxylate of 7-tocopherol.The kinetic results of of 7-tocopherol reacted in the S9 of male SD-rat liver showed that the optimal time was 45 min; the optimal concentration of S9 5 mg/ml; the optimal concentration of NADPH 1 mM; and the optimal concentration of 7-tocopherol 8 μg/ml. The result of γ-tocopherol metabolized in the S9 of male SD-rat was the best; male Brazil turtle better; and male cobia worst.

本试验首次确立了γ-生育酚代谢物的RP-HPLC分析方法,结果理想且重现性好;用GC/MS对γ-生育酚的未知代谢物结构进行分析,判断其中一种主要的代谢物为γ-生育酚侧链初级羟基化代谢物;γ-生育酚在雄性SD-大鼠肝S9的代谢动力学表明,本实验条件下代谢反应的最佳时间为45min,S9浓度为5mg/ml,NADPH浓度为1mM,γ-生育酚浓度为8μg/ml;发现γ-生育酚在雄性SD-大鼠肝S9中代谢最高,雄性巴西龟次之,雄性军曹鱼最低。

Methods In selecting incept concentration of taking of sensitivity, to do mix-skin-test basing on tradition commend concentration and choosing the best concentration to begin to take of sensitivity.

方法在选择脱敏起始浓度时,在传统推荐浓度的基础上,行混合皮试,择取最佳浓度开始脱敏

The results indicated that the concentration of Cu in the soil is 220.16 mgkg^(-1) contrast to the CK 500.31 mgkg^(-1) significantly decrease when the concentration of EDTA in the soil is 2 mmolkg^(-1) that explains EDTA treatment significantly decreased soluble Cu content in soil because of translating into Cu of water distill floating on the surface of soil and most accumulating around root. Contemporary, application of EDTA to the soil significantly enhanced mobilization of Cu in soil and increased Cu uptake in shoot of Leersia hexandra Swartz., the highest concentration in the leaves 336.54 mgkg^(-1) is 4 times higher compared with the ck 80.34 mgkg^(-1). However, citric acid, oxalic acid and malic acid inhibited mobilization of Cu and less significantly affected shoot Cu accumulation.

结果表明,当向土壤中施加质量摩尔浓度为2mmolkg^(-1)的EDTA时,土壤中Cu质量分数为220.16mgkg^(-1)与空白土壤中500.31mgkg^(-1)相比明显减少,说明EDTA可以极显著的降低土壤中的铜质量分数,使其转化成水提取态的Cu浮于土壤表面,并大部分聚集在根部周围,同时向土壤中施加EDTA不但促进了对Cu的活化而且显著提高了李氏禾对Cu的吸收,叶中最高质量分数达到336.54mgkg^(-1)是对照中80.34mgkg^(-1)的四倍;而柠檬酸、草酸和酒石酸则抑制了Cu的活化,但对李氏禾地上部分的Cu质量分数影响不大。

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