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Through the comparison and balance of the treatment effects and economic rationality, the thesis put forward a series of treatment techniques of anaerobic digestion-ANAMMOX -SBR, with consideration of the the high concentration of organic substance , ammonia nitrogen and malodor characteristic of swine wastewater.

通过比较和权衡处理效果及经济合理性,并针对养猪场废水有机物和氨氮浓度高、恶臭严重等特点,提出了厌氧-厌氧氨氧化-SBR的处理工艺。

The morphological analysis of strain EMZY-1 showed that it was a straight rod, about 0.3×1.7 in size, and spherical and terminal spore appeared. Results of physiological and biochemical test showed that strain EMZY-1 was a Gram-positive bacteria, and could not grow when temperature was lower than 10℃ or higher than 60℃, pH was lower than 5.0 or higher than 12.0, and the concentration of NaCl was more than 10%. The strain EMZY could grow on the medium as a of sugar, glucose and mannite. The strain could use saccharose, glucose and mannitol as carbon resource, and NH4(superscript +) and NO3(superscript -) as nitrogen resource.

通过对该菌株的形态、培养特征、生理生化特征的研究以及16S rDNA序列分析表明:该菌株为革兰氏阳性菌;细胞为棒杆状,端生孢子,大小约0.3×1.7;温度低于10℃、高于60℃无明显生长;pH低于5.0、高于12.0无明显生长;NaCl质量浓度达到10%菌株不能生长;能在蔗糖、葡萄糖、甘露醇为C源的培养基上生长;NH4、NO3为菌株良好N源。

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示: VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性;经4 mmol/L VPA处理MUTZ1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞浆内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示:VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性;经4mmol/LVPA处理MUTZ-1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞装内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

With a quantitative research on the chemical and clay compositions of marly stones, their weathering rocks and residuals, as well as SEM observations, the process of dissolution of CaCO 3 and concentration of clay minerals with complex...

通过泥灰质岩石及其不同风化岩和残坡积土的化学成分、粘土矿物成分定量研究和扫描电镜观察,揭示了 Ca CO3溶蚀和粘土矿物富集过程及粘土矿物的复杂成分,发现了风化过程中伊利石向伊利石/蒙脱石混层粘土矿物的转化,解释了岩土工程性质恶化的原因,从而提高了地质研究和地质灾害研究的水平。

With a quantitative research on the chemical and clay compositions of marly stones, their weathering rocks and residuals, as well as SEM observations, the process of dissolution of CaCO 3 and concentration of clay minerals with complex components has been disclosed, and the conversion of illite to mixed-layer illite/montmorillonite discovered, which explain the reason for deterioration of geotechnical properties of the rocks and soils, thereby enhancing the level of geological and geohazards researches.

通过泥灰质岩石及其不同风化岩和残坡积土的化学成分、粘土矿物成分定量研究和扫描电镜观察,揭示了CaCO3溶蚀和粘土矿物富集过程及粘土矿物的复杂成分,发现了风化过程中伊利石向伊利石/蒙脱石混层粘土矿物的转化,解释了岩土工程性质恶化的原因,从而提高了地质研究和地质灾害研究的水平。

Experiments were carried out to study minimum bactericidal concentration of 5 kinds of disinfectants (double fouthdiamine sait-iodine solution, compound aldehyde, chlorine-containing disinfectant; sodium hydroxide and potassium permanganate) against 5 kinds of pathogenic bacteria, and the above MBCs of disinfectants were 1: 600-1: 800,1:1000-1: 2500,1: 30000-1: 40000,0.01%-0.03% and 0.0008%-0.004%, respectively. It emphatically evaluated the sterilized efficacy of each kind of disinfectant to salmonella at different temperatures.

为了解目前猪场常用消毒剂对猪场常见肠道致病菌的杀菌作用,开展了5种猪场常用消毒剂(特碘、复合醛、消特灵、氢氧化纳和高锰酸钾)杀灭5种猪场常见肠道致病菌(沙门氏菌、大肠杆菌、奇异变形杆菌、弗氏拘橼酸杆菌和绿脓杆菌)最低浓度的试验,依次为l:600~l:800,1:1000~l:2500,l:30000~l:40000,0.01%~0.03%,

Objective:To study the extraction in the processes of Weitongliying Soft Capsule after extracted by Supercritical carbon dioxide fluid.Methods:The most appropriate technology was screened based on the comparative study among the technology of ethanol circumfluence,ultrasonic extraction,water warm immersion and water decoction,with the concentration of menstruum, volume and extracting time as the evaluating factors,on the basis of orthogonal design.

目的:研究胃痛立应软胶囊制备中二氧化碳超临界流体(SFECO2)萃取后药材的提取工艺方法:对比乙醇回流、温浸、超声及水煎等提取工艺,以溶媒浓度、用量、提取时间研究因素,经正交设计进行提取工艺条件的优选。

The analysis of the samples collected from the wastewater treatment plant showed that the recycled supernatant had a pH of 7.16~8.21, a conductivity of 371.1~769.1 μmho/cm, a total dissolved solid of 193.3~399.9 mg/L, and a turbidity of 0.901~54.3 NTU. The suspended solids of the recycled supernatant was found to be 0.4~45.6 mg/L, lower than the standard value in the Effluent Standards (50 mg/L). The ammonia nitrogen (NH3-N) and the Total Organic Carbon of the recycled supernatant were found to be 0.06~1.5 mg/L and 1.533~17.437 mg/L, respectively. The analysis of the Chemical Oxygen Demand of the wastewater treatment plant showed a concentration of 12.1~128.5 mg/L in the sample of the recycled supernatant.

在废水处理单元采样分析结果方面,回收之上澄液之pH范围为7.168.21间、导电度值范围在371.1769.1 μmho/cm间、总溶解固体量范围在193.3399.9 mg/L间、浊度范围则在0.90154.3 NTU间;在悬浮固体方面,其回收上澄液之范围在0.445.6 mg/L间,且回收水之悬浮固体均小於放流水水质法规标准值50 mg/L;在氨氮(NH3-N)方面,回收上澄液之范围在0.061.5 mg/L间;在总有机碳部份,回收上澄液之浓度范围在1.53317.437 mg/L间;而在净水场废水处理单元化学需氧量分析结果上,可发现净水场在回收上澄液之样本部份其浓度在12.1128.5 mg/L间。

A substrate concentration of 0.277 kg/L, enzyme/ substrate level of 1408 U/g, temperature of 39.2 ℃, and a hydrolysis time of 4.86 hour were found to be the optimum conditions for high antibacterial activity. Under this condition, the bacteria Escherichia coli, Micrococcus luteus and Bacillus subtilis in the hydrolysates had the inhibitory rate of 71.40%, 56.88%, and 64.54%, respectively.

经Design Expert 7.1.2软件优化,得出胃蛋白酶酶解鲶鱼骨蛋白的最优条件为:底物浓度0.277kg/L,加酶量1408 U/g,水解温度39.2℃,水解时间4.86 h,在此条件下获得的水解物,对大肠杆菌、藤黄微球菌及枯草芽孢杆菌的抑菌率分别为71.40%、56.88%和64.54%。

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