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From ultrastructure of RBC which co-cultured with HAP|,HAP4,HAP5,we have observed that HAPi and HAP4 can induce RBC membrane to deform and decrease stability of RBC membrane, so the particles can enter intramembrane, as a result, intracellular hydroxyapatite nanoparticles may heighten the concentration of Ca2+ and effect structure of membrane protein, membrane lipid and hemoglobin, subsequently,the deformability of RBC decreased all the better and leaded to erythrocyte aggregation. However,HAP.s uniformly dispersed around cell and didn't adhere to it,also, cytomorphosis cannot be found.

观察HAP_1,HAP_4,HAP_5与细胞作用后红细胞的超微结构中,HAP_1与HAP_4引起红细胞膜变形和膜稳定性下降,粒子进入胞内,进入胞内的HAP纳米粒子可能升高胞内Ca~(2+)浓度,从而影响膜蛋白、膜脂、血红蛋白的结构,使红细胞变形性进一步下降,最终导致聚集的发生。

It is found that the concentration of sulfuric acid in liquid and the reaction temperature are the most sensitive factors for this process. Increasing the ratio of solid to liquid is disadvantageous for growth of crystals even though it does not effect obviously for the rate of dehydration of FGD gypsum. On the other hand,the more closed to the neutral range of pH value the liquid is adjusted,the better stability of the α-form calcium sulfate hemihydrate appear,the more favorable for modification of crystal habit the process is made.

研究中发现pH值和盐溶液浓度是影响FGD石膏脱水速度最敏感的因素,固液比虽然对脱水速度影响不大,但固液比增大对晶体生长和习性改良不利。pH值愈接近中性范围,愈能增强α半水石膏结晶在液相中的稳定性,有利于结晶习性改良。

The results indicated that there was no significant difference in hemolymph proteins between irradiated individuals and the controls. Only the concentration of 70kD proteins in irradiated silkworms were observed higher at day 0 of the 5th instar and lower at the later stage of the 5th instar than those of the respective controls.

但在成分分析时发现,5龄起蚕血淋巴中70kD附近的3条蛋白质谱带比对照蚕的浓度要高,随着个体的发育两者的浓度都上升;5龄后期则相反,对照蚕的浓度比照射蚕高;脂肪体中贮藏蛋白质的含量具有相似的变化趋势。

The animal tests indicated that: the levels of plasma and hepatic LPO were elevated in the rats with hyperlipidemia and hypercholesterolemia and at the same time, the red blood cell osmotic fragility and the concentration of SGPT were also in- creased; when the α-Toc was given, the LPO levels of serum and hepar could be de- pressed, the RBCOF and the level of SGPT were reduced too, which suggessted that the membranes of RBC and hepatic cells might be damaged by LPO.

动物实验表明,具有高脂、高胆固醇血症的大鼠血清、肝组织的LPO亦增高,同时红细胞渗透脆性,SGPT也增高,给予α-Toc可以降低血清和肝组织LPO的浓度,RBCOF,SGPT也下降,提示LPO可能引起了红细胞和肝细胞膜的损害。

In iron-deficient cells, photosynthetic pigments content decreased, efficiency of photosynthesis was low, With the increase of the concentration of the iron-chelator 2, 2'-Dipyridyl in media, Fv/Fm decreased, indicating photoinhibition increased, the results were consistent with previous research; Heterocyst induction had one delayed time dependent on the status of iron-deficiency, More severe of the iron-deficiency condition, more late of the heterocyst induction, It is possible that certain iron-related genes may directly regulate heterocyst development; under different iron limitation conditions, Protein pattern were different, more serious of the iron-deficiency conditions, less bands of the protein pattern, We could not find the iron-deficiency induced protein; In general the toxic effects of ROS were potentiated by excess iron, But our results revealed that even under iron-deficiency conditions, Oxidative damage existed.

铁限制的细胞光合色素含量下降,光合作用能力下降,随着铁螯合剂2,2'-dipyridyl浓度的增加,叶绿素荧光F〓/F〓逐渐降低,显示光抑制变强。与前人的研究结果相似;依赖铁缺失状态,异形胞诱导有一段推迟的时间,铁缺失越严重,诱导越迟,推断某些铁相关的基因会直接调节异形胞发育;在不同铁营养条件下,蛋白质图谱的变化趋势很明显,缺铁越严重,则蛋白质谱带数愈少,但本文未能找到特异诱导蛋白;一般认为铁过量情况下ROS产生加强,本文结果显示即便在铁缺失条件下,也有氧化胁迫伤害,而且缺铁越严重,活性氧伤害越大。

While the use of synthetic LaTeX continues to increase, at the most recent full LaTeX lines of glue has been developed, and the development of high-concentration of coatings are the same.

而合成乳胶的使用比例在不断增加,最近全乳胶系的接着剂也被开发出来,这和涂料的高浓度化发展是一致的。

It was found that catalyst activity of USY-1 was the highest under weight ratio of H3PW12O40/USY-1=1∶1,the concentration of H3PW12O40 of 25%,the temperature of 480 ℃ and the time of 4 h.

通过对催化剂制备的正交试验,优选了催化剂的制备条件:H3PW12O40与USY-1质量比为1∶1,H3PW12O40浓度为25%,480 ℃焙烧4 h制备的催化剂活性最高。

We coupled the IgG of monoclonal antibody N34 specific to UK and the Fab of monoclonal antibody SZ-51 specific to the membrane protein GMP-140 of activated platelet. The bispecific antibody (N34 SZ-51) is able to bind UK and plasma-clot simutaneously. It enhanced plasma clot lysis 5 fold by HUK whereas it enhanced plasma clot lysis 38 times by LUK. UK in association with the bispecific antibody didn't decrease the concentration of fibrinogen in the assay supernatants.

利用抗体介导使纤溶酶原激活剂定向到血栓表面,可大大提高其纤溶作用,我们用双功能团试剂将抗尿激酶的单克隆抗体N34的IgG和抗活化血小板膜蛋白GMP-l40的单抗SZ-5l的Fab片段通过二硫键共价偶联,合成的双专一性抗体(N34-SZ-51)既具有血栓亲和性也具有尿激酶亲和性,从而增加了尿激酶对血栓的专一性,使高分子量尿激酶溶栓率提高了5倍,使低分子量尿激酶溶栓率提高了38倍之多,且不影响血浆中纤维蛋白原的含量。

The molecular structural formula of the rhodamine 6G hydrazide salicylal azomethine is shown on the right, and the present invention discloses the synthesization method of the rhodamine 6G hydrazide salicylal azomethine and rhodamine 6G hydrazide salicylal azomethine solution used for the content determination of a small amount of cupric ions in water sample: 0 percent to 98.99 percent of ethanol, 99.99 percent to 1 percent of acetic acid / sodium acetate buffer solution with the pH value of 5 to 9, in which the total concentration of acetic acid is 1 to 100 millimoles per litre, 0.01 percent of N, N-dimethylformamide and 1ppm to 100ppm of rhodamine 6G hydrazide salicylal azomethine, all measured in mass percentage.

罗丹明6G酰肼水杨醛希弗碱,分子结构式如右,本发明公开了罗丹明6G酰肼水杨醛希弗碱的合成方法及其用于水样中微量铜离子含量测定的溶液:0~98.99%乙醇,99.99~1%pH值5~9的醋酸/醋酸钠缓冲溶液,该缓冲溶液中醋酸总浓度为每升1~100毫摩尔,0.01%N,N-二甲基甲酰胺和1~100ppm罗丹明6G酰肼水杨醛希弗碱,均为质量百分含量。

Using the length of cells' growth cycle,the density of cells and the activity of induced hydrogenase as criteria,the optimal formulation of culture medium was obtained as seTA(se -Tris Acet) which contains acetic acid as C source,higher concentration of N and stronger pH buffer system based on the se formulation.

结果发现,培养基的pH和氮素的含量是影响莱茵衣藻生长的重要因素;而培养基中醋酸根的含量则有助于提高厌氧产氢过程中衣藻的氢酶活性,从而提高其产氢量。

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