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The purified N protein was used to coat 96-well plate, each step was optimized, such as coating concentration of recombinant nucleocapsid protein, scample dilutim, chromogen and stop solution as well as concentration.

在此基础上，以纯化的重组N蛋白作为包被抗原，对各种条件进行优化（如抗原的包被，作用时间及底物的选择），确定了判定标准，建立了检测PRRS抗体的间接ELISA方法。

Methods①The minimum inhibitory concentration and the minimum fungicidal concentration of citral against C.

方法①参照NCCLS M27A方案中的微量液基稀释法测定柠檬醛和氟康唑对原始白念珠菌的最低抑菌浓度和最低杀菌浓度；②采用多步诱导法，观察在含梯度浓度(1～50MIC)柠檬醛培养基上连续转种的白念珠菌MIC的变化情况，以氟康唑作为阳性对照，同时设空白对照和溶媒对照；③将敏感性下降的诱导株在无药培养基上连续转种10次后，检测其MIC值；④测定柠檬醛对氟康唑敏感性下降诱导株的MIC值。

So Strain M12-69 is a potential strain, which can be used to produce RFR with high concentration of monacolin K and low concentration of citrinin.

因此，应变的M12 - 69是一种潜在的压力，这可以用来制造RFR高浓度的莫纳可林K和低浓度的桔霉素。

Compared with previous papers (Sabater-Vilar et al., 1999; Xu et al., 1999), RFR produced by M12-69 has higher concentration of monacolin K and lower concentration of citrinin.

与此前的文件（ Sabater -维拉尔等。， 1999年；徐等人。， 1999年）， RFR生产的M12 - 69具有较高浓度的莫纳可林K和低浓度的桔霉素。

Collector current is proportional to concentration of dopamine in the range over 5.0×10〓mol/L to 5.0× 10〓mol/L and remains independent of ascorbic acid concentration to approximately 5. 0×10〓mol/L.

由于在收集电极上消除了由电位变化引起的充电电流和阴极-阳极工作时的反馈作用，多巴胺的检测限可达7.5×10〓mol/L，线性范围为5.0×10〓～5.0×10〓mol/L。

In order to reduce the composite probability of Cd and O, simultaneity , to enhance the Cd-N covalent characteristics and improve the hole concentration, it is important to control the proper concentration of Cd and O.

研究表明共掺体系中，适当控制Cd和O的浓度，可以减小Cd和O的复合机率，加强Cd-N的共价特性，对改善AlN的p型特性有重要意义。

Methods Exerting backwards force on rats mandible and measuring concentration of rats' blood cAMP in three groups with radio-immunity. Drawing the time-concentration curve and making cosine analysis.

给大鼠下颌戴向后上牵引的矫形器，用放射免疫法测定不同加力组大鼠血中环磷腺苷含量，绘制时间-浓度曲线，并进行时间生物学的余弦统计分析。

The photolysis decoloration rate increases with the concentration of H2O2, but, with a high concentration of H2O2, the improvement is not obvious.

染料光解脱色速率随H2O2浓度的增加而增加，但在高H2O2浓度时，降解速率增加不明显。

Objective: To study different concentration of sodium sulfide depilatory which have different degree injury to new zealand rabbits skin and to find the small injury and good unhairing concentration.

目的：探讨不同浓度硫化钠脱毛剂对新西兰家兔皮肤的损伤程度，试图找到对实验动物皮肤损伤小、脱毛效果佳的脱毛剂浓度。

The results show that, the static interfacial polymerization was conducted in organic phase. The optimized conditions for NF membrane preparation were obtained: the concentration of PDMAEMA was 2%, the concentration of p-xylylene dichloride was 1 ~ 1.5%, NaHCO_3 can be used to maintain alkalescence, The polymerization can be carried out at room temperature and the reaction time was 5 hour.

研究结果如下：此界面聚合反应在有机相中进行；较优的制备条件为：PDMAEMA浓度为2%，对二氯苄浓度为1～1.5%，应加入少量的NaHCO_3来维持溶液的微碱性，室温下反应即可进行，反应时间为5小时。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！