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When the other conditions were stable, the effect of different concentration of the microinjected DNA, and different timing of micromanipulation in CZB medium, different quality of zygotes on microinjection was investigated in vitro. Results: The higher the concentration of the microinjected DNA, the worse the development of zygotes.

获取小鼠受精卵，在其他条件固定的情况下，分别比较不同的注射基因浓度，在CZB操作液中显微操作时间以及注射前受精卵的质量对显微注射后的胚胎体外培养结果的影响。

Nine wethers selected from a group at 2-years-old of age were fed with fresh mixtures of Leymus chinensis (30%), Cleistogenes squarrosa (20%) and Artemisia frigida (50%) in known proportions and amounts for 14 days. Artificial QSM capsules were dosed during the experiment. The samples of herbage and feces were collected daily for 7 days and dried for analysing the n-alkane concentration by a gas chromatography. The results showed that there was a difference in concentration of n-alkanes among 3 species forages, and fecal recoveries of n-alkanes linear increased with increasing carbon chain lengths.

于2004年秋季在中国科学院内蒙古草原生态系统定位研究站用不同放牧演替阶段的优势植物羊草、糙隐子草和冷蒿按一定比例混合组成日粮，饲喂9只2岁羯羊，每只羊投喂一粒QSM胶囊，试验期内每天记录绵羊实际牧草采食量、采食成分和排粪量，利用气相色谱分析牧草和粪样的链烷含量，应用链烷技术测定绵羊的排粪量、不同牧草采食比例和总干物质采食量，并与实际值进行比较。

In typeⅡdiabetes mellitus,the concentration of free fatty acid and triglycerine is generally high, especially in obesity.Long-term exposure ofβ-cells to high concentration of FFA will induceβ-cell s apoptosis. Many studies have shown that glucose and FFA effect synthesis and secretion of insulin stimulated by the insulin secreted byβ-cells trigged by themselves.

并进一步对功能受损的β细胞进行分子生物学检测，通过观察游离脂肪酸在不同浓度葡萄糖协同作用下对PI-3K、P-PKB、PDX-1活性及表达的影响来揭示这种协同作用导致β细胞功能损伤的分子机制。

The compatibility of both Gvirens and B.firmus was researched. The fermentation filtrate of Gvirens , under the concentration of 70%, had not distinct inhibitory activity to B.firmus, the biomass of B.firmus would decrease with above the 70% filtrate of Gvirens, but the fermentation filtrate of B.firmus had not inhibitive role to Gvirens. 7, The cooperation control of both Gvirens and B.firm to the pine needle blight was explored:(1) Pathogen inoculation happened prior to the bio-control treatment for 7 days, however, the disease index and control effect had not distinct differences in any treatments even if combination of the two antagonists, but the susceptibility for different kind of pine were different.(2)when antagonist and pathogen were inoculated at the same time, some control effect to the pine needle blight, including certain extent cooperation control of both Gvirens and B.firm were proved, but it wasn't distinct when two antagonists used separately, and the change of the antagonist concentration had little effect to the control effect.

G.virens与B.firmus对松赤枯病的协同控制显示：(1)预先接种病菌后(7天)再进行生物防治处理，不管那种情况，感病指数和防治效果在处理间差异不明显，浓度影响无显著差异，即使是两种生防制剂联合作用也未显著提高其防效，但不同树种间的感病性有明显差异；(2)领抗菌与病原菌同时接种，对松赤枯病有一定防治效果，两菌联合有一定的协同作用，两菌分别单独施用防效差异不显著，浓度变化对防效影响不大；(3)预先接种颇抗菌能显著提高防治效果，两菌联合处理防效更优，感病树种的感病指数可降至13一15，抗病树种的感病指数可控制在3%左右；在同一浓度下B.f1'r功US、叹叮厂即s间无显著差异，在同一领抗菌下，浓度愈高，防效愈高：松，(4)三种生防接种方式的平均效果分析表明，叹F行ens最高平均防效49.5%黑 B。

Cells and tissue damaged will also occur when cultured fish live inhigh concentration of nitrite environment.With the concentration of nitriteincreasing,the activity of antioxidase will decrease,especially in headkidney,spleen and liver of cultured fish immune relatively organ.

不同浓度的亚硝酸盐对机体的影响实验结果表明，随着环境中亚硝酸盐含量的上升，机体内各器官抗氧化物酶的活力极显著下降，SODp＜0.05；CAT p＜0.05；而体内的脂肪氧化代谢产物MDA含量显著升高(p＜0.05)，头肾中的MDA与正常组相比，其MDA的含量极显著上升(p＜0.05)。

The twig production of different clones is different, from 5.5 twigs per month to 14.8 twigs per month. The rooting rate, root number and root length change regularly as the concentration of IBA goes up from 0.05 to 2.00 mg/L. At the concentration of 0.05 mg/L ppm IBA, rooting rate and root length are the highest. Cutting substrate also has eminent effect on rooting rate and survival rate, 50% peat plus 50% sub-top soil is the best substrate. The survival rate differs greatly among clones, from 56.7% to 90.0%. Rooting rate and survival rate change regularly as season changes. The rooting rate of May and June is middle, that of July and August is high and that of September and October drops rapidly. The survival rate is low in May, September and October and high in June, July and August.

结果表明：不同无性系的萌条数量有显著差异，高的达14.8条／月，低的5.5条／月，平均9.1条／月，从0.05~2.00 mg/L，随着IBA浓度的提离，生根率、根条数、总根长等生根指标均呈现有规律的变化；综合考虑各种生根指标，以IBA0.05 mg/L为最佳生根激素处理，扦插介质对柚木插穗生根有显著的影响，以泥炭土＋黄心土按1：1混合为最佳扦插介质；不同无性系的扦插成活率不同，从56.7%至90.0%，变动幅度大，随扦插季节变化，生根率和成活率均呈现有规律的变化，5~6月份的生根率中等，7~8月最高，9~10月份迅速下降，成活率随季节的变化也是两头低、中间高，6~8月的成活率最高，大于93%。

The range of few cytotoxic concentration of Napp varied from 0μg/ml to 1000μg/ml and at concentration from 10μg/ml to 1000μg/ml could significantly decrease the level of intracellular RNA of HCV subgenomic replicon cell modelP

体外实验无细胞毒药物浓度区间为0～1000μg/ml，在10μg/ml～1000μg/ml区间内对HCV复制子RNA有明显抑制作用P

The effects of concentration of complexing agent isooctyl alcohol and different dilution agents (kerosene, toluene and methyl-phenol) on extraction efficiency, coefficient of distribution and phase splitting time were investigated. The best extraction result was achieved with toluene as dilution agent and the concentration of complexing agent isooctyl alcohol of 0.5.

论文研究了络合剂异辛醇浓度、不同稀释剂对萃取率、分配系数和分相时间的影响，当甲苯作为稀释剂，络合剂异辛醇浓度为0.5时萃取效果最好。

The results showed that lower concentration of the NaCl had scarcely any influence on the regenerative velocity. Higher concentration of the NaCl caused death of regenerating planarians, KCl has stronger poisonosness towards regenerating planarians, CaCl2 (0.1% and 0.3%) could increase the regenerative velocity.

结果表明：跟对照组相比，低浓度氯化钠对涡虫再生速度影响不大，高浓度氯化钠引起涡虫解体；氯化钾对再生涡虫的毒性较大；0.1%和0.3%氯化钙可以提高涡虫的再生速度。

In the first cultivation stage,as relatively high concentration of phosphorus,phosphorus from water column was removed to a certain extent by periphyton.The end of the incubation,as low concentration of phosphorus periphyton releases phosphorus to water column instead of removal phosphorus from water,this may closely relate to the alkaline phosphatase secreted by periphyton in water body.

从培养过程中水体磷的浓度变化来说，培养初期，水体中磷的浓度比较高，附着生物对其有一定的去除作用；培养后期，水柱中磷的浓度比较低，附着生物不但不去除水中的磷，而且还向水柱中释放磷，这可能与附着生物向水体分泌碱性磷酸酶密切相关。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！