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The chemical structure of the product was characterized by means of the melting point and IR. The results show that tetraethylammonium bromide which was used as a phase transfer catalyst in this reaction had optimization effect on the interfacial diffusive reaction of BTC and PNP. Higher concentration of sodium hydroxide, lower temperature and a higher dosage of BTC produced a small amount of 4-nitrophenyl chloroformate, while lower concentration of sodium hydroxide and a lower dosage of BTC made reaction incomplete, which affected the yield.

结果表明,在碳酸二(4-硝基苯基)酯合成中使用的相转移催化剂四乙基溴化铵对受界面扩散控制的BTC气与对硝基酚钠反应有良好的相转移催化效果;氢氧化钠质量分数较低时,酚钠盐向溶有BTC的油相扩散速度较小,较高时会产生氯甲酸对硝基苯酯;温度较低、BTC量较高时,会产生少量氯甲酸对硝基苯酯,BTC用量较低会使反应不完全,这些都影响着收率。

Results The IC50 of SPG-Rg3 was (155.7±0.71) mg·L-1,when the concentration of SPG-Rg3 was arranged from 37.5 to 600.0 mg·L-1,the growth inhibitory rate of MCF-7 cells was higher following the increase of its concentration,the cell growth was greatly inhibited compared with control group(P.05); cell cycle was changed,the cell number of S period increased compared with control group(P.01),the cell number of G2/M period decreased compared with control group(P.01),in experiment group there was an apoptotic apex before G1 apex,and the number of apoptotic cells increased; most of cell nuleus in SPG-Rg3 (150 mg·L-1)experiment group appeared yellow or chrysoidine fluorescence,contracted,beaded and crescent; the result of immunocellularchemical staining of caspase-8 indicated that caspase-8 protein had no expression in control group,but stronger expression in SPG-Rg3 group.

结果:SPG-Rg3 IC50为(155.70±0.71) mg·L-1,当SPG-Rg3浓度在37.5~600.0mg·L-1时,MCF-7细胞的生长抑制率随SPG-Rg3浓度的增加而增大,与对照组比较,细胞增殖受到明显抑制(P.05);MCF-7细胞的生长周期也发生变化,S期细胞数增加,明显高于对照组(P.01,G2/M期细胞数则明显少于对照组(P.01;并且在G1峰前出现明显的凋亡峰,凋亡细胞数增加。

Study the activity of inducible nitric oxide synthase in the liver of rabbits infected with Eimeria stiedai, the concentration of nitric oxide in the serum、the coccidian oosysts per gram feces ,Score the pathologies according to anatomic structures of rabiits'livers and micoscopic pathological changes. So as to study the effect of exogenous NO and NOS of the rabbits infected with E.stiedai. The results showed that when the rabbits were infected with E.stiedai, the activity of iNOS in the liver and the concentration of NO in the serum gradually increased and they both reached the peak after 12 days in the control group , GTN and GSNO groups.

为了探讨外源性一氧化氮和一氧化氮合酶在家兔球虫感染时的作用,本试验以腹腔注射的途径给予感染斯氏艾美耳球虫的家兔NO供体硝酸甘油和亚硝基谷胱甘肽,对球虫感染家兔诱导型一氧化氮合酶活性、血清中NO的浓度、每克粪便的球虫卵囊数、肝的剖检和镜下病变及其病变计分进行了研究。

The influences of technic conditions of preparation, such as concentration of sodium silicate, concentration of vitriol, reaction temperature and cocurrent time were studied by orthogonal design experiment.

利用正交设计安排实验,采用并流法制备沉淀二氧化硅,研究实验制备工艺条件硅酸钠浓度、硫酸浓度、反应温度和并流时间对沉淀二氧化硅的BET比表面积的影响。

When the concentration of MAH is higher than that of St, some MAH monomer reacts with St to form SMA, others can directly grafts onto PP macroradicals. When the amount of St added is higher than that of MAH, part of St monomer may preferentially react with PP macroradicals to form more stable styryl macroradicals, while others copolymerize with MAH, yielding SMA. MFR of the grafted PP is lower in this case. It is considered that MFR of the grafted PP is decided by concentration of free monomers, or, number of grafting reactive species. FTIR analysis confirmed the above mechanism.

当MAH单体用量多于St单体时,一部分MAH单体与St反应生成SMA,另一部分可直接与PP大分子自由基进行接枝反应;当St单体用量多于MAH单体时,除与MAH反应生成SMA之外的St单体可先与PP大分子自由基反应,并起到稳定PP自由基的作用,这时接枝物的MFR较小,亦即接枝物的MFR与体系中可进行接枝反应的活性种数目有关。

At a concentration of 10~(-7) M/10~6 cell, cUMP elicited an increase of cGMP level by 38.3%(p.005), without affecting the concentration of cAMP.

在10~(-7)M/10~6细胞cUMP作用下,胞内cAMP水平几乎没有变化,而cGMP水平升高38.3%(P.005)。

A one-year investigation on the N and P concentration and accumulation of Cyperus malaccensis in the wetlands of Minjiang River estuary showed that the N and P concentration of C.

对闽江河口湿地优势植物短叶茳芏N、P养分含量与积累量进行了1年的监测。

By means of the transmission electron microscope we found the typical morphology variation of apoptosis,such as the concentration and side-accumulation of the nuclear chromatin,the fragmentation of the nuclear and the concentration of the cytochylema etc.Cell cycle detected by flow cytometry showed one visible apoptosis peak (20.6%) in front of the peak of G1.The typical ladder was found in the 15 g.L-1 agarose gel electrophoresis of the nucleus DNA.

透射电镜下可见细胞核染色质浓缩、边集、核碎裂及胞质浓缩等凋亡细胞典型的形态学改变;流式细胞术周期分析显示在G1期峰前存在一个凋亡峰(20.6%);核琼脂糖凝胶(DNA 15 g.L-1)电泳呈梯状。

The toxicity of trichlorphon would become degradation after a week under the same conditions. For example, the survival rate of Daphnia pulex reached to 100 percent in the concentration of 200μg/L after 144h and 65 percent in the concentration of 400μg/L after 168h.

在相同的实验条件下,敌百虫溶液的毒性可在一周后降解:如200μg/L的敌百虫溶液在放置144h后,蚤状溞的存活率就达到100%;168h后,400μg/L的存活率为65%。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞,用100 TCID50柯萨奇B3病毒(CVB3)感染心肌细胞,建立病毒性心肌炎实验模型,然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞,观察心肌细胞的形态和搏动,用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白,四唑蓝比色法测定心肌细胞活性和病毒抑制率,流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

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