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OBJECTIVES: To predict duration of azotemia and distinguish acute renal failure clinically from chronic renal failure by measuring the concentration of hair creatinine and analysing the correlation between Hcr and serum creatinine in 59 patients with acute renal failure and 64 patients with chronic renal failure .

目的:通过对59例急性肾功能衰竭(Acute renal failure,ARF)病人和64例慢性肾功能衰竭(Chronic renal failure,CRF)病人头发肌酐含量(Hair creatinine concentration,Hcr)测定、比较ARF组和CRF组病人就诊时30-40mm段头发肌酐含量之间的差别、对Hcr与血肌酐浓度(Serum creatinine,Scr)相关关系的研究,在临床上利用头发肌酐含量测定来判定肾功能衰竭持续的时间,进一步鉴别急、慢性肾功能衰竭。

It will also be the keystone of a new strategy of civic boosterism for the former mining town, which will soon be able to boast the largest concentration of work by Le Corbusier outside Chandigarh in India.

对于这个昔日的采矿城市而言,它亦将成为公民自主建设新政策(new strategy of civic boosterism)的典范。而这座城市,将能够自夸为除印度昌迪伽尔以外现存柯布的作品规模最大、最集中的欧洲城市。

The trial model with chicken encephalomalacia was set up by using the feed of low congtent of V〓 and supplying excessive unsaturated fatty acid in feed for studying the molecular mechanism of chicken encephalomalacia in the study. On the basis of observing the symptom and postmortem examination in clinic and determining the content of V〓, the bodyweight, the weight of cerebrum and cerebellum, the focus of the study was on follows which were relative to neuropathic injury. The concentration of free radical and the function of antioxidant defense system of chicken with encephalomalacia were determined systematically.

中文题名雏鸡脑软化症的分子机理副题名外文题名 The molecular mechanism of chicken encephalomalacia 论文作者王振勇导师石发庆教授学科专业临床兽医学研究领域\研究方向动物营养代谢病学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数110页关键词雏鸡脑软化鸡馆藏号BSLW /2003 /S858 /7 为了深入探讨雏鸡脑软化症发病的分子机理,本课题通过在采用低V〓饲料和添加过量UFA建立了雏鸡脑软化症实验模型。

These essentially comprise a constant test volume flow, a uniform velocity profile for the air over the filter's face area, and a temporally constant concentration of test particle size.

这些本质上包括一个恒定的测试容量流,一致的速度空气的profile在过滤器的面孔区域和临时地恒定的集中 of试验粒子大小。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实:两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用,并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著,最佳效应浓度为400nM;2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响:①CREG蛋白对VSMC迁移的影响:刮伤实验发现,加入最佳效应浓度的wtCREG和mCREG蛋白24h后,OB2组迁移能力下降,HITASY组无明显变化;细胞外基质金属蛋白酶-2,9(Matrix metallo-proteinase 2,9,MMP2 ,9)明胶酶电泳检测和Western blot检测结果证实,两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2,9减少,而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases,TIMPs)增加;②CREG蛋白对VSMC分化的影响:加入400nM的wtCREG和mCREG蛋白12h后,OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加,LM-1、FN表达减少;③流式细胞仪分析细胞周期和BrDU染色分析证实,加入400nM的wtCREG和mCREG蛋白后,OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572,HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034;3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用:①免疫共沉淀和免疫荧光双染色分析结果显示,CREG蛋白与M6P/IGF2R存在直接结合;②应用抗体阻断实验:将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中,CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱,而且与加入anti- M6P/IGF2R浓度正相关。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003~2007年临床分离的220株Pa,对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况,同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration,MIC),筛选出对亚胺培南耐药的铜绿假单胞菌,并分析其对其它抗生素的药物敏感率;采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株,采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因,采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因,用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况;同时对产AmpC酶的Pa(25株,含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

The results showed that at the anaerobic phase, the PHB concentration in the cell and orthophosphate concentration of the anaerobic effluent obviously increased, the poly-P concentration in the cell decreased, and the cucci and bacilli with a size of 1.0~1.5 μm were dominant and formed grapy cellular cluster. At the anoxic phase, the PHB concentration and orthophosphate concentration decreased significantly, the amount of poly-P increased, and the elliptical cucci of 0.5~1 μm and spheral bacilli of 1.0~1.5 μm were dominant. At the aerobic phase, the PHB and orthophosphate concentration almost reduced to zero, the poly-P increased significantly, cucci appeared single or in pair, and the contour of sphere was blur and almost disappeared.

试验结果表明,在厌氧阶段,微生物细胞内PHB的量大幅提高,上清液中磷酸盐的含量上升,聚磷酸盐含量明显下降,粒径为1~1.5 μm、呈球状和棒状的菌群构成的葡萄状细胞簇占居优势;在缺氧阶段,微生物细胞内PHB的量下降,上清液中磷酸盐含量下降,聚磷酸盐含量上升,粒径为0.5~1 μm的椭球菌与1.0~1.5 μm的球杆菌占优势;在好氧阶段,微生物细胞内PHB的量较低或接近零,上清液中磷酸盐的含量接近零,聚磷酸盐含量明显上升,此时粒径1.0~1.5 μm的球菌以单个或成对出现,球菌不再饱满,呈现接近消失的状态。

Results For single treatment groups,serum drug concentration of chlorpromazine and clozapine were related to age of patients (P<0 05),concentration of clozapine was related to dosage (P=0 01).For combination group,concentration of chlorpromazine was related to dosage (P<0 01),also related to scores of PANSS and general psychopathological symptoms (P=0 01),concentration of clozapine was related to scores of PANSS,negative and general psychopathological symptoms (P<0 05).Multiple linear regression analysis showed that forms and methods of treatment affected concentration of chlorpromazine,age of patients and methods of treatment affected concentration of clozapine.

结果 单用氯丙嗪和采用氯氮平组的血药浓度均与年龄相关(P<0 05),氯氮平血药浓度与剂量线性正相关(P=0 01);在双氯组中的氯丙嗪血药浓度与剂量线性正相关(P<0 01),与PANSS总分及一般精神病理分相关(P=0 01),而氯氮平血药浓度与PANSS总分、阴性量表分及一般精神病理分明显相关(P<0 05);多元回归分析显示,治疗形式和治疗方法影响氯丙嗪血药浓度,年龄和治疗方法影响氯氮平血药浓度。

Var. pyramidalis is not positively correlated with ion concentration in growth environment, the capacity of selective absorption and transport of ions in Populus alba L. var. pyramidali under salt stress in drainage canal is higher than Populus alba L. var. pyramidal is of normal growth in irrigation canal. Under salt stress, the concentration of K(superscript +)、Na(superscript +) of roots and leave of Populus alba L. var. pyramidalis have changed, the concentration of K(superscript +) of roots and leave dropt the concentration of Na(superscript +) of roots markedly increased, the concentration of Ca(superscript 2+), Mg(superscript 2+) have slightly changed, the concentration of Ca(superscript 2+) of roots dropted, the concentration of Mg(superscript 2+) increased, the concentration of Ca(superscript 2+), Mg(superscript 2+) of leave increased.

主要结论为:新疆杨各器官的离子含量与生长环境中的离子浓度不存在正相关趋势;排渠盐胁迫下生长的新疆杨对离子选择吸收和运输的能力高于灌渠正常生长的新疆杨;盐胁迫下,新疆杨根和叶中K、Na的浓度发生了明显的变化,K浓度在根和叶中呈下降趋势,Na浓度在根中明显增加,根和叶中Ca(上标 2+)、Mg(上标 2+)浓度的变化趋势略缓,根中Ca(上标 2+)浓度下降,Mg(上标 2+)浓度增加,叶中Ca(上标 2+)、Mg(上标 2+)浓度有所上升。

It is revealed that natural diamonds may contain as much as 59 different trace elements. The concentration of each trace element is above 1 ppm(10〓). However, the major impurities are nitrogen, hydrogen, oxygen and boron. The study of the forms of hydrogen in diamonds not only enriched the contents of mantle mineralogy, material sciences and gemology, but also provided some important informations for dealing with some problems concerning the Earth's mantle and core.

中文题名金刚石中氢的赋存状态研究副题名外文题名 The existing states of hydrogen in diamonds 论文作者杨志军导师谢先德研究员彭明生教授学科专业矿物学、岩石学、矿床学研究领域\研究方向学位级别博士学位授予单位中国科学院广州地球化学研究所学位授予日期2002 论文页码总数10,113页关键词空隙氢晶格氢金刚石馆藏号BSLW /2003 /P57 /1 天然金刚石中的微量元素达59种之多,其含量都在10〓级规模以上,且最主要的杂质元素为氮、氢、氧和硼等。

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