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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Later advancements saw the ends of the can made out of aluminum instead of steel. In 1962, Ermal Cleon Fraze of Dayton, Ohio, invented the integral rivet and pull-tab (also known as rimple or ring pull), which had a ring attached at the rivet for pulling, and which would come off completely to be discarded.

之后的技术进步经历了铝替代铁做原材料使之能够冷凝。1962年，来自俄亥俄州代顿的艾马尔·克里昂·弗拉泽﹙Ermal Cleon Fraze﹚发明铆钉和拉标签：铆钉上外套一个环锚紧，拉开后可整个被丢弃。

Is how Konrad Steffen, a climatologist at the University of Colorado, put it last week, releasing the results of a new study. The Arctic sea ice is shrinking so fast that within the next few decades, if not years, it could be completely gone in summertime.

北极的海冰正在飞速消融，照此速度，在今后几十年里，可能就在某一个盛夏时节，它们将完全融化。

The change of cloaca configuration is related to the differentiation of rectum and bladder; however, it is inappropriate to emphasize this change too much while completely deny the role URS plays.

虽然泄殖腔构型的变化也参与了直肠与膀胱分离的过程，但过份强调泄殖腔构型的变化而完全忽视甚至否定尿直肠隔对泄殖腔的分化作用是不恰当的。

At first has been unable from the city to countryside Miyori to adapt the local life completely, looks like in her, not the familiar paternal grandparents, the cloddish school and the student, all look like are such are incompatible with.

最初由城市到了乡下的Miyori完全无法适应当地的生活，在她看来，并不熟悉的祖父母、土里土气的学校和学生，一切看起来都是这么的与自己格格不入。

"Playing soccer" the post-game title is "Cloe Ze and Borowski brings the attractive goal"(schone tore von klose und borowski), the article analyzes: In the German cup second round, Bayern has won "Bavaria Derby" the victory, Kelinsiman this competition changes four guard's tactics, this has also obtained the success, if is not the Bayern luck is unsatisfactory, they have the possibility to obtain more goals completely, but in Berry also celebrated his this season to present for the first time.

踢球者》赛后的标题则是"克洛泽和博罗夫斯基带来漂亮进球"（schone tore von klose und borowski），文章分析到：在德国杯第二轮，拜仁赢得了"巴伐利亚德比"的胜利，克林斯曼本场比赛变回了四后卫的战术，这也取得了成功，如果不是拜仁运气欠佳，他们完全有可能取得更多的进球，而里贝里也庆祝了他的本赛季首次亮相。

When I asked a man near me about it, he explained that they were Carmelites, members of an order that lived a cloistered existence completely apart from society.

我向旁边的一名男子打听其中的原因，他告诉我说，她们是加尔默罗会修女，按会规，这个会的人生活在一个完全与世隔绝的修道院里。

Rock'n'Roll circle is a home-like place, people are very closed, just like a group of brothers that are completely bound to each other.

"摇滚圈是一个像家一样的地方，人和人之间特别亲，像一帮相依为命的穷兄弟。"

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。