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On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明,所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达;在缺乏AMBN情况下,与对照组相比,实验组牙胚在体外可以继续生长发育至钟状晚期,出现成釉细胞和成牙本质细胞的分化,成釉细胞可以分化成为分泌期型成釉细胞,胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体,缺乏溶酶体,表明对蛋白合成和脚的能力降低;实验组牙胚有牙尖形成和基质分泌,但牙尖形态异常,基质形成减少,牙尖周围基质最厚处为O.6卜m,明显薄于对照组的5.spin,基质中胶原纤维粗细不等,排列稀疏, 3 第四军医大学硕士学位论文未见钙化现象,充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程,影响胶原纤维和牙本质基质的合成,促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

Coculturing of the transfected fibroblasts with neonatal rat ventricular myocyte cultures resulted in a significant reduction (68%) in the spontaneous beating frequency of the cultures compared with baseline values and cocultures seeded with naive fibroblasts.

与基线值和原始的成纤维细胞的再复制相比,乳鼠心室肌细胞培养基里转录的成纤维细胞的再繁殖,导致了培养基里自律性频率的显著下降(68%)。

Coculturing of the transfected fibroblasts with neonatal rat ventricular myocyte cultures resulted in a significant reduction (68%) in the spontaneous beating frequency of the cultures compared with baseline values and cocultures seeded with naive fibroblasts.

结果发现,与基线、与幼稚成纤维细胞共培养相比,转染的成纤维细胞和新生大鼠心室肌细胞共培养导致培养物自发搏动频率的下降(68%)。

Compared with Simvastatin Tablets and the capsule of Xuezhikang,the effects of Wendan decoction on serum triglycerides,serum total cholesterol,serum LDL-C and serum HDL-C in adult rats with chronic hyperlipidemwere observed after30dates.Result:By using pharmacological meth-od,the results revealed that Wendan decoction could effectively reduce serum of TC,TG,LDL-C levels in the rats with chronic hyperlidemia,it has raisen the concents of blood HDL-C and its function was more effective than the capsule of Xu-ezhikang.

除空白对照组喂基础饲料外,其余各组均采用高脂乳剂灌饲方式建立大鼠高脂血症模型,喂养10日后眼球取血,测得受试动物血清中总胆固醇、总甘油三酯、低密度脂蛋白胆固醇显著升高,说明高脂血症模型复制成功。30日后,运用药理学方法,观察温胆汤对高脂血症成年大鼠血清中TC、TG、LDL-C、高密度脂蛋白胆固醇含量的影响,并与西药辛伐他汀、中成药血脂康进行比较。

Per urinary tract transitional cell carcinoma in patients. Methods:Urinary Fn was detected by ELISA technique in 30 cases with upper urinary tract TCC, 24 cases with other urogenital diseases and 19 healthy subjects. Meanwhile the analysis for creatin in urine was carried out and the Fn/Cr ratio was calculated. The discrepancy of urinary Fn/Cr among three groups and tumor grades and stages was analized as well. Sensitivity and specificity of the urinary Fn/Cr were compared with that of the urinary cytology.

采用ELISA法检测30例上尿路移行细胞癌、25例其它泌尿系疾病患者及20例正常人尿液Fn含量,并同时测定尿肌酐,计算Fn/Cr比值,比较各组人群尿Fn/Cr比值之间的差异;分析不同肿瘤分期、分级尿Fn/Cr之间的差异;比较其与尿脱落细胞学检测上尿路移行细胞癌的敏感性。

Foliar sprayed with 500μmoll〓FePS has showed better regreening effects in dicots compared with root supplied with 1μmoll〓〓FePS.

对双子叶植物而言,叶面喷施FePS的效果要好于根施1μmolL〓的FePS,而对单子叶植物根施的效果要明显好于叶面施用。

It's usually fastened with clips and nails to a metal frame of certain size used for seats or beds; Compared with unstable elastics, it is tensile and flexible with high endurance; moreover, it largely increases the comfort of products. That's why it is widely used in furniture industry.

它一般用平钩和螺丝安装在一定大小的铁架上,作为整个座椅或者床的一部分;与其他产品相比,S簧具备更强的的伸张力、弹性和更久的疲劳性能,并且极大地增加了底座的舒适度,这就是为什么它会成为家具行业的首选配件的原因。

Experimental results indicate that the B/A value greatly affects the internal Nusselt number and friction factor of the twisted tube because the parameters decrease with the increase of B/A and S/d values, more rapidly with the former, and that the Nusselt number and friction factor of the twisted oblate tube increase respectively by 30%~150% and 20%~50%, as compared with those of the smooth tube at the same Reynolds number and Prandtl number.

实验结果表明:高扭曲比螺旋扁管的管内努塞尔数和阻力系数均随B/A值和S/d值的增大而减小,但随B/A值的增加而减小得更快,B/A值对高扭曲比螺旋扁管的管内传热与流阻性能影响更大;在相同雷诺数和普朗特数下,高扭曲比螺旋扁管管内的努塞尔数为光滑管的1.3~2.5倍;阻力系数为光滑管的1.2~1.5倍。

Gear shaper cutter with convex rake face with hard-tooth-face can improve the ability of anti-collapse efficiently while guarantee the shape precision compared with traditional gear shaper cutter.

硬齿面凸曲前刀面插齿刀和传统插齿刀相比能够在保证构形精度的前提下有效提高刀齿的抗崩刃能力。

The fatigue strength of duplex stainless steel welded jointing with longitudinal fillet welded gusset and with longitudinal flat side gusset welded on plate were evaluated by hot spot stress,and the hot spot stress S-N curves were obtained and compared with the nominal stress S-N curves.

针对双相不锈钢焊接结构的疲劳性能问题,通过试验对双相不锈钢纵向角接板焊接接头和侧面角接板焊接接头疲劳强度用热点应力进行了评定,做出了热点应力S N曲线并与名义应力疲劳评定进行了比较。

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