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objective to compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on the skin of d-galactose-induced mice aging model.methods 60 female mice were randomly divided into 4 groups (n=15):mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of d-galactose[1000 mg/(kg1·d)] for 42 d to establish subacute aging model.the experimental groups included agingmodel group,lycium barbarum polysaccharides [200 mg/group,tanshinone [1 500 mg/] group.forty two days later,the dorsal skin samples were collected to determine sod activities、mda and hydroxyproline contents.
60只雌性昆明种小鼠,随机分为4组(n=15):正常对照组皮下注射生理盐水;其余3组每日颈背部皮下注射 d-半乳糖[1000 mg/]造成小鼠衰老模型,同时各组分别灌胃生理盐水、枸杞多糖[200 mg/]、丹参酮[1 500 mg/]。42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。
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Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.
本论文在上述研究成果的基础上,选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型,进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制,探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制;以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制;以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响,从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。
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Random cent is 120 patients that the method excises appendix of lens of travel abdominal cavity the operation observation group and contrast group, each 60, contrast group give convention to nurse, observation group restores to hold in the application on this foundation take exercise, compare 2 groups of bowel to cry sound restore time, anal exhaust time and costive occurence rate.
方法将行腹腔镜阑尾切除手术的120例患者随机分为观察组和对照组,各60例,对照组给予常规护理,观察组在此基础上应用恢复操锻炼,比较2组肠鸣音恢复时间、肛门排气时间及便秘发生率。
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Cruciferae vegetable crop s were chosen to compare their resistance to cadmium contamination because of many hyper-accumulation plant species reported belong to this family.
选择目前报道富集植物相对比较集中的十字花科蔬菜作物,采用室内水培方法,观察镉对种子萌发及早期幼苗生长的抑制程度,确定其毒性效应。
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To search the genetic heterogeneity of ADPKD and to compare the clinical presentation between ADPKD type 1 and 2 in Shanghai Han population, develop a method of gene diagnosis by linkage analysis with microsatellite DNA tightly linked to PKD1 and PKD2 and make gene diagnosis of mutation carriers before cystogenesis, detect single nucleotide polymorphism of PKD2 and its frequence in Han population.
根据知情同意的原则,选取80名无亲缘关系的上海市汉族健康志愿者进行与PKD1和PKD2连锁的微卫星DNA多态性的研究,选取43个常染色体显性多囊肾病家系进行遗传异质性、表型差异研究及基因诊断。
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objective:with the local lobster shell as the raw material,we inquire into the abstraction and preparation of chitin and chitosan to develop a new raw material resources for chitosan.methods:use methods such as take off protein,calcium,colour,deacetylate and purification to prepare chitosan to study deacetylate degree,molecular weight and other characters of chitosan,then compare with infrared spectrum of bio-chemical chitosan on market.results:the chitin and chitosan obtained from lobster shell are 14.8% and 8.9%, respectively.
目的:以地产龙虾壳为原料,探讨其甲壳素的提取和壳聚糖的制备方法,以开发壳聚糖制备的新原料资源。方法:用脱蛋白、除钙、脱色、脱乙酰基、纯化等方法制备壳聚糖,研究其壳聚糖的脱乙酰度、分子量等性质,并与市售生化级壳聚糖的红外光谱进行比较。
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Customers' and service employees' opinions about the critical incidents which impacts on service encounters of fast-food restaurants and deluxe restaurants were collected, coded, and analyzed under the dramaturgical theory and Bitner's standard. It is to compare customers' view with service employees' and to identify the critical factors to service encounter satisfaction of fast-food restaurants and deluxe restaurants in Taiwan.
所发展出的分类原则两种标准,进行多次的分类归纳,比较顾客与服务人员两方面对影响服务接触满意之关键因素看法的异同,并确认对於台湾的速食业与高级餐厅而言影响服务接触满意的关键因素为何,及此关键因素是否会因行业别的不同而产生差异。
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laser of co2 of lattice of τ肊 ncore activefxtm caves to 40 place sexual acne scar has treatment 2 times, the watch of ecca weight grading that treats around to use b.dreno has grading statistic to scar place, compare its curative effect. result: soul of acute communicable diseases holds?
应用encore activefxtm点阵co2激光对40处凹陷性痤疮瘢痕进行治疗二次,治疗前后使用b.dreno的ecca权重评分表对瘢痕部位进行评分统计,比较其疗效。
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Enteritidis)strains in Shanghai from 2006 to 2007.[Methods] A retrospective analysis from 2002 to 2007 was performed to explore the source of food-borne S. Enteritidis strains and compare its antibiotic resistance with strains isolated from diarrhea patients.Data of S.
方法回顾2002—2007年肠炎沙门菌食品株的来源并与肠炎沙门腹泻株作耐药性比较;对2006—2007年全球沙门菌监测病例和食品分离的肠炎沙门菌进行表型验证、药敏试验和脉冲凝胶电泳分析。
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Objective1 To observe the biofilm formation on urinary catheters from critical patients.2 To analyse the risk factors of biofilm formation on urinary catheters from critical patients.3 To compare the eradicative effects of different irrigation methods in vitro.
目的1观察重危患者留置尿管表面生物被膜的形成情况。2分析重危患者留置尿管表面BF形成的危险因素。3比较体外不同冲洗法在清除尿管表面BF中的作用。
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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.
曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。
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The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.
稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。
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When I was in school, the rabbi explained everythingin the Bible two different ways.
当我上学的时候,老师解释《圣经》用两种不同的方法。