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The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜,采有用酶消化法和组织培养法获取成骨细胞体外培养并传代,观察细胞形态,生物特点及成骨特性,并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径,将不同浓度的钛合金颗粒(1mg/ml,0.1mg/ml,0.01mg/ml)与成骨细胞共同培养,分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml,0.1mg/ml,0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

In the present study, full-length rDNA sequences from the domestic and wild type of Chinese oak silkworm, Antheraea pernyi (GenBank No. GU073314 and GU073315, respectively) were obtained and comparatively analyzed on their genetic variation.

测定了放养型和野生型柞蚕的rDNA ITS-2全长序列(GenBank登录号:GU073314、GU073315),并分析了二者之间的遗传差异。

The conclusion shows that Anthracene is the main compound in the extracts from water solution of YM coal oxidation. The contents of Hexadecanoic acid、Anthrone and 9,10-Anthracenedione are comparatively high. Aromatic and nitrogenous heterocyclic compounds were also detected, which shouldn't be neglected.

结果表明:在所得水溶液的苯可溶物中检测出绝对高含量的蒽;含量较高的棕榈酸、蒽酮和蒽醌;另外还有一些微量的芳环、杂环类化合物和一系列的长链烷烃。

amusement news accept and welcomed with interest and irritating their by people.though theory circle yet have finalconclusion by amusement to news at present,audiences'essential demand of psychology offers the demand for news amusement production,official silent approvement of will news amusement realistic terms that phenomenon can store in continuously.therefore the amusement of the tv news rises gradually,the comparatively swift and violent growth momentum appears.at the same time,we must take strict precautions against the trend of vulgarization urgently.

娱乐化新闻因其趣味性和刺激性逐渐被人们接受并受到欢迎。尽管理论界目前对新闻娱乐化尚无定论,但受众的心理本质要求为新闻娱乐化的产生提供了需求,而官方意志的默许则是新闻娱乐化现象能够持续存在的现实条件。因而电视新闻娱乐化逐渐兴起,并呈现较为迅猛的发展势头。与此同时,媚俗化倾向亟待加以防范。

Comparatively accurate colorimetric effect could be obtained when to use armillary light as light source and to ensure control group and test group were located in symmetrical location of the center.

不同方向的光会对数码色度值造成影响,使用环形灯作光源,并保证对照色标和被测色标处于画面中央对称位置则可以获得较为准确的比色效果。

The chromatic value were the same basically.Conclusion:The light of different direction would affect the chromatic value. Comparatively accurate colorimetric effect could be obtained when to use armillary light as light source and to ensure control group and test group were located in symmetrical location of the center.

不同方向的光会对数码色度值造成影响,使用环形灯作光源,并保证对照色标和被测色标处于画面中央对称位置则可以获得较为准确的比色效果。

Our experiment has indicated that high-dose epinephrine for resuscitating patients with asystole has got the comparatively goo...

实验提示:对心搏停止的病人,大剂量肾上腺素复苏取得了较好的效果;而对室颤病人,大剂量用药效果不确切。

Our experiment has indicated that high-dose epinephrine for resuscitating patients with asystole has got the comparatively good result,but the e...

实验提示:对心搏停止的病人,大剂量肾上腺素复苏取得了较好的效果;而对室颤病人,大剂量用药效果不确切。

Our experiment has indicated that high-dose epinephrine for resuscitating patients with asystole has got the comparatively good result,but the effect of usi...

实验提示:对心搏停止的病人,大剂量肾上腺素复苏取得了较好的效果;而对室颤病人,大剂量用药效果不确切。

Furthermore, cell culture and amplification are comparatively easy so we can get a great quantity of cells applied by autografting and allografting.

目的:探讨由脂肪组织分离获得脂肪基质细胞的方法及其在体外经诱导培养后向成骨细胞分化的潜能。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。