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Objective: To identify and distinguish common staphylococcal species by developing a real-time PCR assay with melt curve analysis.

目的： 建立一种基于葡萄球菌16s～23s rRNA间隔区序列特征的荧光定量PCR/熔解曲线分析鉴定。

Therefore, it can be regarded as a great improvement of the investment decision-making theory and methods .This article makes a systematic research on real options theory and method and its application, including the following several main aspects:Firstly, it makes a detailed discussion about the common theoretical basis of financial options and real options, including no-arbitrage equilibrium principle, risk-neutral definition, complete market and dynamic tracking.Secondly, it analyze three basic characteristic of investment decision-making in modern market economic system: irreversibility, uncertainty and flexibility, and from the angle of their mutual quantitative and qualitative interactions, it makes a comparison between the traditional NPV methods and real options methods and makes a summary of the general analytical methods of real options.Thirdly, it discusses the all elements in the application of real options such as the ^leakages in value of real assets, basis risk, and private risk, and furthermore, it attempts to put forth a normalized strategy of its application and construct a basic framework of its application.Fourthly, it applies real options theory to strategetic investment of enterprises and the buy-out of company, putting forth a value model for strategetic investment program and making a concrete analysis of its application to buy-out. Furthermore, it advocates that a flexible investment decision-making system should be setup according to real options theory in our country.

本文对实物期权理论和方法及其应用进行了系统研究，主要内容包括如下几个方面：(1)详细讨论了金融期权和实物期权共同的理论基础，主要包括无套利均衡原理、风险中性定义、完全市场以及动态复制方法，对这些理论方法的探讨既是本文的出发点和基础，它们提供了研究实际应用实物期权的一个基准点，也是贯穿全文的线索和灵魂；(2)分析了现代市场经济体系中投资决策的三个基本特征即不可逆性、不确定性和时机选择，并从它们数量上和质量上相互作用的重要意义的角度，分析了传统的净现值法与实物期权方法的差异，指出了净现值法在不确定环境中存在的缺陷及实物期权处理投资灵活性的价值，并将净现值法纳入实物期权投资分析决策体系当中，总结了企业投资决策实物期权的一般分析方法；(3)探讨了实物期权应用中要考虑的各种要素，如实物资产的价值漏损、基差风险和非市场风险等，并进一步提出了实物期权应用的规范化策略和构建了一个实际应用实物期权方法的基本框架，尝试将期权的基本原理与企业在现实经济环境中投资决策的要素结合起来，从而可以在一定程度上超越期权的复杂的数学计算而汲取期权思想的精华，从而促进期权决策方法在实际投资决策中的应用；(4)将实物期权方法应用于企业战略投资，得出了战略投资项目的价值模型，指出可以将实物期权理论和方法应用于企业并购，分析了应用的具体方面，并提出应根据实物期权理论建立一套适合我国企业的灵活的投资决策系统。

Considering gas-solid flow in annular space and BoYun Guo's hydrodynamic parameters model, For aired underbalanced drilling, combined with the minimum energy Angel law and based on the mininum gas injection scale at the key points, it is established a bottom-hole pressure model for gas-solid two phase stream; meanwhile, calculated the downhole depth of the Downhole-Deployment-Valves; and then fabricated the corresponding calculation software. For streamingly underbalanced drilling, it is fully given a step-by-step solution based on analysis from the relationship of various wellbore pressures at the course of underbalanced drilling as well as from the streaming behavior in different wellbore sections, adding consideration on multiphase stream pressure drop and Downhole-Deployment- Valve into account, it is of common application for development wells.

对于气体欠平衡钻井，考虑环空为气固两相流，参考BoYun Guo的流体力学参数计算模型，结合Angel的最小动能法标准，从满足关键点处的最小注气量入手，建立了气固两相流的井底压力计算模型，同时对套管阀的下入深度进行了计算，并利用VB语言编制了相应的计算软件；对于液体欠平衡钻井，考虑环空为多相流，在充分分析欠平衡钻井井内基本压力关系的基础上，从环空多相流压降分析入手，通过对井筒内各段的流态分析，逐段求解。

The use of unbonded tendons is now common in prestressed concrete structures, and they may be in the form of internal or external tendons.With the increasing use of unbonded tendons in both new construction and retrofitting of existing structures, there is the need for a closer look at their design and analysis.

采用无粘结预应力是现在常见的预应力钢筋混凝土结构，并且它们可能采取的形式是内部或外部tendons.with越来越多地使用无粘结预应力在这两个新的建设和改造现有的结构，有必要建立更密切看看他们的设计与分析。

4Basis on the analysis of the risks of arbitrage on stock index future in China, there are lots of risks induced by the unconsummated spot market besides common risks.

需要套利交易者引起重视以及谨慎判断现实交易过程中的套利机会。

Secondly, taking unstiffened plate as an example, the paper applies finite element analysis to checking the validity of buckling assessment equivalent formulae of CSR including JTP and JBP, carries out comparison between JTP and JBP Rules in buckling strength assessment, and analyzes the main reasons for error. The paper also provides reference for the harmonization of Common Structural Rules.

其次，以非加筋板为例，应用有限元方法验算了共同结构规范中对屈曲强度进行等效计算的有效性，并比较了共同结构规范中JTP规范和JBP规范对屈曲强度校核的不同点，分析了规范中造成误差的主要原因，为规范的进一步调整提供了参考。

Bimetal strip is one of the most common structure in the field of electrical appliance and other fields but the traditional analysis method for working characteristics is not systematic and complete and is not easy to analyze all parameters.

摘 要：条形双金属片是电器及其他领域中应用最多的结构型式之一，其传统的工作特性分析方法在理论上欠系统与完整，且不便于分析其全部参数。

The article makes the analysis of one shallow to the common issue that buy a house from legal angle, in helping the person that buy a house answer the process that buy a house a variety of ambuscading.

本文从法律的角度对常见的购房纠纷作一粗浅的分析，帮助购房者应对购房过程中的种种埋伏。

Then he analyses all factors which are relatively with tourism real estate, then he colligates the key factor and common factors and establishes the preparing target system. Then he uses the method of relativity analysis to filtrate the targets, constitutes textual system. He adopts arrangement analytical method to ensure each target's tentative weight.

同时，对影响旅游地产项目开发模式选择与评价的各方面因素进行分析，综合各种开发模式的关键因素和各方面因素，我们建立了旅游地产项目开发模式选择与评价的备选指标体系，在运用相关性分析和定性分析等方法对指标进行筛选之后，确立了本文的指标体系。

This article starts form carding the formation and development of the animations in the United States, Japan and Europe, in their respective national television animation style characteristics. In the second chapter, from the national culture, folk customs and ways, the character and geographical four conditions explore the United States, Japan, Europe, ethnic style animation in the film formation due to the dynamic; Chapter III trays to find the common aesthetic features of the Television Animation factors in United States, Japan, Europe, also this part describes the characters from four aspects, such as to find truthfulness, to find kind and so on. Chapter four is the key of this article, this part colludes several parts , such as film animation level, the way how animation to present to the concept of integrity, the character and language of the text character and so on . This end of this article is a summary of papers, down from globalization understanding of the vision of China Television Animation, the theoretical study of the ultimate goal is to practice creative services, what is more important is a comparative analysis, which received some of reference, identify China Television Animation and the gap between other countries, in order to learn from foreign advanced development of the film and television animation experience in the domestic film and television animation themes and concepts updates, in the localization of the close integration with the international on the road to the former OK, can have a more far-reaching significance.

论文首先从美、日、欧、中影视动画各自民族风格的特点出发，对其民族风格的形成与发展进行系统的梳理；进而在第二章中，从民族文化、民风民俗、民族性格和地域条件四个方面探究美、日、欧、中影视动画民族风格形成的动因；第三章是对美、日、欧、中影视动画的同质性因素进行比较，论文从启真、扬善、弘美、尚趣四个维度来寻求其共通的美学特征；第四章中外影视动画民族风格的异质性因素比较是本文的重点，也是论文的难点，这一章涉及到许多影视动画本体性的层面，拟从动画的观念品格、文本品格和行为品格三个方面进行把握、比较；最后一章是论文的总结，从全球化的视野下来认识中国影视动画，理论研究的最终目的是要为实践创作服务的，因而，论文更主要的是通过比较分析，从中获得一些借鉴性意见，找出中国影视动画和其他国家之间的差距，更好的学习国外影视动画先进的发展经验，以期对国产影视动画在题材和观念的更新上、在本土化与国际化紧密结合的道路上如何前行能够有更为深远的指导意义。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。