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A steel bar is washed and dried, and then put into a glass pipe which is sealed to obtain stirring magneton; a mixed solvent of Gamma-methacrylate oxide propyl trimethoxy silane and methanol is prepared and added with an organic base to obtain a pre-bonding solvent; the stirring magneton is immerged into the pre-bounding solvent, and the outer surface of the glass pipe of the stirring magneton is bonded with a layer of silane substance with alkenyl; a reactive monomer mixture and a pore-forming agent mixed solvent are evenly mixed and then added into a container, the stirring magneton after the pre-bounding is put into the container which is sealed and then put into a water bath; after reaction, the container is removed, the stirring magneton coated with a polymer is taken out and put into a extractor and extracted by using a solvent; or the stir bar is immersed into the solvent till no impurity can be checked in the solvent, thereby obtaining the product.

将铁条清洗干燥，放入玻璃管内，密封得搅拌磁子；配制γ-甲基丙烯酸氧丙基三甲氧基硅烷与甲醇的混合溶液，加入有机碱得预键合溶液，将搅拌磁子浸入预键合溶液，在搅拌磁子的玻璃管外壁键合一层带烯基的硅烷化物质；将反应单体混合剂和致孔剂混合溶液混匀后倒入容器中，将预键合后的搅拌磁子放入容器，密封后置于水浴中，反应后除去容器，取出涂有聚合物的搅拌磁子，置于提取器中，以溶剂提取，或将搅拌棒置于溶剂中浸泡至溶剂中无杂质检出，得产物。

Dis- Fly away lonely souls into the endless sky so far away When you're all alone Feeling down and lonely Tell me what you see deep inside your soul You only know who you are So don't be afraid Just open your heart to me You will spread your wings like an eagle Learning how to fly in the sky Just like the sun rises after the rain you'll shine in the air Take off your mask and be true to your heart trust yourself and fly away Into the light of joy and truth Where there's a grief there will always be a hope For a place where we can be who we wanna be Your tears may fade away But always keep the faith and live tomorrow Break through the night away into a brand new day Life's just begun Yeah, feelin' the melody of eternity It has the power to set us free Travlin' through the galaxy with only space around Follow the suns peaceful sound It's time for us to end this illusion Fightin' ourselves will only cause confusion Shinin' light is the key of life Let's all get together and make this better I don't know how but there has got to be a way There are times when I Wish I could just disapear Far from this place of pain and hurt If only I could be gone There's something in your eyes Pulls me back from running away Oh, yes it's hard sometimes But now I'm strong enough to tell the sadness goodbye Just like the moon fills the night with the stars I'll dance in the air Seek in the darkness there's nothing to fear I will stand and fly away Into the night I'll find the truth The pain never ends but there's always be a hope To be free eternally in this galaxy To live another day the sun will show the way and I will follow Break through the night away into a brand new day My journey goes on Into the light of joy and truth Where there's a grief there will always be a hope For a place where we can be who we want to be Your tears may fade away But always keep the faith and live tomorrow Break through the night away into a brand new day Your journey's begun

阴间的神-飞孤单的灵魂进入在如此远处的永无止境的天空之远之内当你全部很孤独的时候感觉下来和孤单的告诉我你在你的灵魂里见到起来深的你只知道你是谁如此不害怕仅仅对我开放你的心你将会像一只鹰一样传布你的翅膀学问该如何在天空中飞仅仅在雨之后喜欢太阳上升你将会在空气中发亮脱掉你的假面具而且忠于你的心信赖你自己和飞行之远进入欢喜和事实的光之内哪里总是有伤心事在那里意志是一个希望对于我们能是的一个地方我们想要当谁你的泪滴可能褪色离开但是总是保存信心和活的明天进入商标新的日子之内夜晚之久突破仅仅被开始的生活是的，feelin'永恒的曲子资讯科技有力量释放我们 Travlin'经过银河由于只在附近隔开跟随太阳和平的声音资讯科技是我们结束这一个幻影的时候 Fightin'我们自己只将会引起混乱 Shinin'光是生活的钥匙全部让我们聚在一起而且使这变成更好我不知道如何除了那里之外已经开始是一个方法有时代此时我希望我可以正直的 disapear 离痛苦和伤害的这一个地方很远如果只有我可能不见了某事在你的眼睛中从逃走把我拉回来哦，是的资讯科技努力地有时是但是现在我够强壮来告诉悲哀再见仅仅喜欢，月亮将夜晚装满星我将在空气中跳舞黑暗的找寻没有东西害怕，我将会站而且飞离开进入夜晚之内我将找事实痛苦从不结束但是总是有是一个希望在这银河中是自由地永恒地在另外的一个日子居住太阳将会表示方式而且我将会跟随进入商标新的日子之内夜晚之久突破我的旅程继续进入欢喜和事实的光之内哪里总是有伤心事在那里意志是一个希望对于我们能是的一个地方我们想要当谁你的泪滴可能褪色离开但是总是保存信心和活的明天进入商标新的日子之内夜晚之久突破你的被开始的旅程

The carbons in explosives are decomposed into gas-like carbon atoms in the detonation reaction zone, then unoxidized carbon atoms coagulate into carbon liquid droplets and finally consolidate into solid diamonds The new mechanism differs from one conclusion that carbons of explosives are at first transformed into solid graphites, then into diamonds, and also differs from the conclusion that gaseous carbons coagulate into liquid droplets in the reaction zone, then crystalize into diamonds in Taylor rarefaction wave zone.

修正了有关炸药中的碳在反应区内先转变为石墨，然后石墨再发生相变生成金刚石的论点以及认为在反应区内气态碳凝聚为碳液滴，液滴在Taylor稀疏波区凝固成金刚石的论点。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Hey yo jim man why don't ya, you don't you kick some of that you know you know how you do it man it's a trip people don't even believe were together right now but tell your story you know the one i like say it for me riders on the storm riders on the storm into this house we're born (into this house we're born) into this world we're thrown (into this world we're thrown) like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah goin' off of this off of that with the lizard king bumpin' in the back how bout that driftin', liftin', swiftin', coastin', testaroastin' but the wheels won't stop 200 on the highway fresh up off the block he's a rider, na he's a killer dresses in all black but his hat says stealla pedal to the metal i gotta go hard drive by and say hello hey fredwreck you my mello now let me here what i sound like acapella wow ride dip swish now bring it back just like this like a dog with out his bone unlike a g with out his chrome it's hard to imagine the homey dog in a jag and he's checkin' for the checkered flag comin' in first never in last cause my car to fast i neva eva run out of gas cause i just to clean i do it upper class so fasten your seat belts its so hot it will even make heat melt so get a bowl and roll and ride slip through the slip and slide like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah need for speed i'm trying to take the lead hold on little homey before you run into the trees i've seen thinks that i would have never saw before hey yo jim let'em in, let'em in open up my back tire smokin' the whole street and now the police wanna flash there lights and chase the dogg all night but i won't pull over nor give up cause i just don't give a fuck yeah from the side boy where we was born and raised straight up to ride boy continuously, we get to an expeditiously keep the light on east side on snoop dogg and the doors and yeah we bout to ride on riders on the storm riders on the storm into this house we're born into this world we're thrown were thrown

嘿摇摇弯轨机人为什么不唉，你不你踢一些那你知道你知道如何你这样做人这是一次旅行人们甚至不相信是在一起此时但告诉你的故事你知道那个我像为我说它骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔像个狗没有一根骨头一个演员那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀去'离开对于这离开关于那和蜥蜴国王 bumpin'在后面如何拳击比赛那 driftin'，liftin'，swiftin'，coastin'，testaroastin'但是这个车轮不会停止两百在公路上新鲜向上远离块他的一个骑士，娜他的一个杀手衣服在纯黑但他的帽子说stealla 踩踏板金属我有去难驶过和问好嘿fredwreck 你我的梅洛现在让我这里什么我听起来像acapella 哇骑现在倾斜豪华带来它回来只是这样像个狗与出来像个狗没有一根骨头一演员借出的骑士在暴风雨上那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀需要速度我是试图着先鞭继续小像家一样在你未碰撞树木我已经看到想那我从来不锯在以前嘿摇摇弯轨机让'em 在，让'em 在开发我的后面疲劳smokin'一街一巷那么警察要闪光那儿灯光和追逐道格整夜但我不会拦截也不放弃造成我只是不给一个性交是呀自边男孩我们在那里出生和提升直直至骑男孩骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔我的翻译不太标准别见怪

Put Twisted Sisters on MTV, yeah[01:46.40]Those were the days[01:49.49]I'll come back[01:50.75]To Iron Maiden and 7-Up[01:53.99]Bananarama and Breakfast Club[01:57.35]Come back[01:59.37]Come back[02:00.93]I'll come back[02:03.77]When Huey Lewis was the news[02:07.46]And we went flying with Tom Cruise[02:11.26]When shoulder pads and looking tough, ooh-oh-oh[02:15.26]Was the dirty David Hasselhoff[02:18.64]Too many men with heavy makeup on[02:22.34]Too many poodles with guitars[02:25.97]Thanks for all the joy you've given us[02:29.81]You will always be our superstars[02:35.28]Back to the 80s[02:36.86]Back to Soap[02:38.99]Back to Rocky and Cherry Coke, yeah[02:42.62]Those were the days[02:45.10]And we go[02:46.47]Back to the 80s[02:47.94]Say 'Hey there, Mister T.

无论里根是演员还是总统，[04：35.09]都在80年代留下了无法抹灭的印记。[04：39.15]satellite："卫星"，[04：40.81]在歌词中指的是"orbital satellites轨道卫星"，[04：44.80]这暗指里根时代的 star wars "星球大战"计划，[04：49.21]此计划在克林顿被停止执行）[04：53.80]skinny leather ties：是80年代在欧美国家风行的装束，[04：57.75]男孩们都喜欢戴着细条长的皮质领带，[05：00.89]尤其受到乐队的乐手们的宠爱。

Come on, come on Give me everything I deserve all I can get, you aint seen no-nothing yet I'm gonna grab the brassy golden ring Come on, come on I want everything I know it's gonna happen...

来吧，来吧给我的一切我应该所有我可以，你是不是没有看到，但没有我一定抓住黄铜金戒指来吧，来吧我想要的一切我知道一定发生过。。。

Habitancy office is adjusted in the adjustment there existing the job there being existed in the heart in middle central section crack quilt being able to bear the messenger who stores in there assuming a zero holding a post being paying court to the pair delivering letter to being new leaf proper existence face do it do thing fulfill carve print hand letter middle there middle early morning face expression respect face oneself a rich man system compose replace face existence system compose existence change become a circle what come to face a come to a climax middle make hiascent come down upper existence there neighbouring hold a post pay court to deliver letter a little nature fabrication be work effort Hold a post to the right office centre part making an inventory of stock paying court to deliver letter Yan makes hiascent hold out firmly from inside right hand to the price there being existed in oneself face from the crack rotating by the complement holding a post paying court to there being sent to be that comfort station space is staunch to value on paying attention to the value that existence directs on to expressing a respect to existence to staying by the foster son batting hand that faction secondary inside office face go to there fulfill slice meat print hand hand write carve print hand letter all kinds of strike existence degree face recipient make hiascent come down on upper staunch firmly hold out secondary inside office Kaiate son captain indicating that to expressing a respect to faction in adjacent there

Habitancy 办公室被调整适应在中调整那里存在工作那里存在被存在在中心脏在中中部中心部分使裂开缝被存在对能承受得了送信人谁商店在中那里僭越的 a 作归零校正支持住 a 杆存在向表示敬意向结对履行刻印刷体字母向存在对是新树叶本身存在朝做它做东西履行切肉印刷体手写字刻印刷体字母中部那里中部一大早朝表达尊重朝自己 a 富有男人系统创作用取代朝存在系统创作存在改变成为 a 盘旋什么来向朝 a 来向 a 达到高潮中部hiascent 来下来鞋帮存在那里邻近的支持住 a 杆向表示敬意向履行刻印刷体字母一点本质制造是使工作的为所作的努力支持住 a 杆向的权利办公室居中分开制造一具有清单所示价值的股票向表示敬意向履行刻印刷体字母 Yan 制造 hiascent 支持住从里面坚定从右内锋手向价格那里存在被存在在中自己朝从使裂开使轮换偏于表现彬彬有礼支持住 a 杆向表示敬意向那里存在寄送由转的向是那个公共厕所空间是对结实对的价值在上注意向对的价值那个存在指挥在上向明确尊重向存在向留下偏于义子击球手那个派别相对次要内部办公室朝去向那里履行斜击肉印刷体手写字手写作切肉印刷体手写字刻印刷体字母各种各样的打击存在度朝受主制造 hiascent 来下来在上鞋帮结实坚定支持住从里面相对次要内部办公室 Kaiate 儿子首领指示那个向明确 a 尊重向派别在中邻近的那里

Someone is tossing petals in a stream Somewhere someone is standing at the foothills of their dreams Someone got a paintbrush, is painting over doubts Someone opened up his eyes and saw the sun coming out Someone was captive and found the courage to get off From a boulder in the well, somewhere the rain has stopped Someone is finding the place where they belong Everyday is summer somewhere in the world And the summer boys are headed for the falls to kiss the girls With their impatient hands groping honey breasts and curls They are filled with desire And high in the hills there's a baby being born As forgiveness and peace wash over bruises and sores People bridging the distance over nettles and thorns Everyone aboard on the merry-go-round Some things will rise up so that others come down If the devil don't dance, heaven won't shine It's a mighty thick haze and it's a pretty thin line If the facuet is tightened up the love won't flow If the love isn't bright enough the corn won't grow If the night isn't dark enough the moon won't glow A rich man counting money, a tired man counting sheep While the safe man counts his blessings, the hungry man has beans There's a million people praying, raising up their eyes To what turns out to be the same god, the same sky We are slightly scared of death, a little bit afraid So we celebrate everything we can think to celebrate We shall sing out loud to keep the hounds away Everyone aboard on the merry-go-round Some things will rise up so that others come down If the devil don't dance, heaven won't shine It's a mighty thick haze and it's a pretty thin line If the facuet is tightened up the love won't flow If the love isn't bright enough the corn won't grow If the night isn't dark enough the moon won't glow Prisons will crumble and governments will fall It's the order of freedom to be preceded by walls Cause the truth would be worthless if no one ever lied So we carry our shame in the interest of pride And we have all these questions to make us go roam And we've got all this distance to make us come home As the sun burns, a child learns, the tide churns, the world turns Everyone aboard on the merry-go-round Some things will rise up so that others come down If the devil don't dance, heaven won't shine It's a mighty thick haze and it's a pretty thin line If the facuet is tightened up the love won't flow If the love isn't bright enough the corn won't grow If the night isn't dark enough the moon won't glow

有人扔花瓣在流某处有人站在山脚下的他们的梦想有人了画笔，画的是怀疑有人打开了他的眼睛，看到太阳出来有人圈养和发现的勇气下车从巨石的很好，某处雨已停止有人的地方发现它们属于日常是夏天在世界一些地方和夏季男孩前往瀑布亲吻女孩不耐烦的手与他们摸索蜂蜜乳房和卷发他们是充满希望和高的山上有一个婴儿出生作为宽恕与和平洗了伤痕和疮人民缩小距离，荨麻和荆棘 93号航班上的旋转木马有些东西会起来，使其他人下降如果没有魔鬼舞蹈，天上不会服务这是一个强大的浓雾，这是一个非常薄线如果是加紧facuet的爱将不会流如果爱情是没有足够明亮的玉米不会增长如果是没有黑暗的夜晚足够月球不会发光富翁数钱，一个疲惫的人计数羊虽然安全的人把他的祝福，在饥饿的人有豆有100万人祈祷，提高了他们的眼睛什么结果是相同的上帝，同样的天空我们稍微害怕死亡，有点害怕因此，我们庆祝所能想庆祝我们将唱大声保持距离猎犬 93号航班上的旋转木马有些东西会起来，使其他人下降如果没有魔鬼舞蹈，天上不会服务这是一个强大的浓雾，这是一个非常薄线如果是加紧facuet的爱将不会流如果爱情是没有足够明亮的玉米不会增长如果是没有黑暗的夜晚足够月球不会发光监狱将崩溃和政府将下降有秩序的自由之前，墙壁原因真相将是毫无价值的，如果没有人说谎因此，我们在履行我们的耻辱的利益骄傲和我们所有这些问题让我们去漫游我们还有这一切的距离，使我们回家太阳灼伤，儿童学习，潮流，提供世界轮流 93号航班上的旋转木马有些东西会起来，使其他人下降如果没有魔鬼舞蹈，天上不会服务这是一个强大的浓雾，这是一个非常薄线如果是加紧facuet的爱将不会流如果爱情是没有足够明亮的玉米不会增长如果是没有黑暗的夜晚足够月球不会发光

I'm the Fireman Fire, Fa, Fireman I got that fire I'm hollering I got that fire come and try me and You can spark it up and I'ma put you out You can spark it up and I'ma put you out [Verse 1] Ain't nobody fuckin with me man, Heatman Ski Mask spending next weeks cash, he fast And I don't even need a G pass I'm pass that I'm passing em out now and you can't have that And my chain Toucan Sam That tropical colors you can't match that Gotta be abstract You catch my gal legs open betta smash that Don't be surprise if she ask where the cash at I see she wearing them jeans that show her butt crack My girls can't wear that why, that's where my stash at I put my mack down that's where you lack at She need her candlelit and I'ma wax that I rekindle the flame She remember the name It's Weezy Baby January December the same Mama gimme that brain Mama gimme that good Cause I'm the fireman You hear the firetruck I'm the Fireman Fire, Fa, Fireman I got that fire I'm hollering I got that fire come and try me and You can spark it up and I'ma put you out You can spark it up and I'ma put you out Fresh on campus it's the Birdman Jr Money too long teachers put away ya rulers Raw tune not a cartoon No shirt, tattoos, and some war wounds I'm hot but the car cool She wet that's a carpool Been in that water since a youngin you just shark food Quick Draw McGraw I went to art school Yeah the lights is bright but I got a short fuse Don't snooze Been handling the game so long my thumb bruise Ya new girlfriend is old news Yeen got enough green and she so blue yeah Cash Money Records where dreams come true Everything is easy baby leave it up to Weezy Baby Put it in the pot let it steam let it brew Now watch it melt don't burn ya self I'm the Fireman Fire, Fa, Fireman I got that fire I'm hollering I got that fire come and try me and You can spark it up and I'ma put you out You can spark it up and I'ma put you out Ridin' by myself well really not really So heavy in the trunk make the car pop-a-wheelie Who?

我是消防员消防，花园，消防员我接到火警我喊叫我接到火警来试试我，它可以激发起来，我是把你出去它可以激发起来，我是把你出去 [诗歌1] 不与我没有fuckin人，Heatman 滑雪面具的现金支出今后几周，他快我甚至不需要一个G通过我传递我现在传递时间内，你不能有链巨嘴鸟和我的山姆这种热带色彩不能匹配肯定在抽象你明白我的加仑双腿粉碎的开放斗鱼不要惊讶，如果她问其中现金我看到他们，她穿着牛仔裤，显示她的屁股裂缝我的女孩不能穿这个原因，这就是我的藏匿在我把我的下这就是你缺乏麦克她需要她的烛光和我是一个蜡余点燃圣火她记得这个名字这是12月1月Weezy婴儿一样妈妈gimme，大脑妈妈gimme，良好原因我是消防员你听到救火车我是消防员消防，花园，消防员我接到火警我喊叫我接到火警来试试我，它可以激发起来，我是把你出去它可以激发起来，我是把你出去新鲜校园它是鸟人Jr 钱太长教师收起亚统治者原料调整不是一个卡通没有衬衫，纹身，和一些战争创伤我觉得又热，但赛车冷却她湿这是一个用车在这水自1 youngin你只鲨鱼的食物快速绘制麦格劳我去艺术学校是的，灯光明亮而我得到脾气暴躁不要打盹被处理的游戏，只要我的拇指挫伤亚新女友是旧闻陈吕令意有足够的绿色和蓝色是啊，她这样现金货币记录下梦想成真一切都留给孩子容易到Weezy婴儿放入锅蒸让它让它酿造现在看它融化不烧亚自我我是消防员消防，花园，消防员我接到火警我喊叫我接到火警来试试我，它可以激发起来，我是把你出去它可以激发起来，我是把你出去我自己也实在没有真正因此，在主干沉重让赛车弹出一，离地平衡特技谁？

In contrast to the ubiquitous rising-sun-with-rays military flag of the Japanese, Chinese banners and ensigns feature a range of designs.

与遍地都是的太阳军旗不同，中国人的旗帜和徽章设计得各式各样。

From their small corner of Feng's Guangzhou headquarters -- a jumble of pink leashes, squeezable rubber steaks, and plastic doggy Santas for Fido's stocking -- Soleil's designers come up with at least five new products a month.

从Feng 设在广州总部的产品展示柜台上可以看到，Soleil的设计师每月至少设计出5件新产品。