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Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

Actually network advertisement this thing is very the thing of entirely imaginary, for finite to our financial capacity individual, basically won't have the effect with practical what, perhaps should say effect =o, perhaps you should say those big websites, big door, big forum, advertisement frightens a the dead more, ah, that is much really, but the advertisement effect in the advertisement effect to them and our eye is different, why so say inside, the advertisement effect in our eye, the actualest is to give us to bring clinch a deal quantity, if if clinch a deal,the quantity is no good, retreat and beg next, bring constant flow to also be the silver that him not let sb down spends to us, however those old ad advocate the advertisement great majority that join is to sell a product, the advertisement effect that they ask is to reveal closely, the website that does not need me brings how many discharge, sell go out a few products of what.

其实网络广告这个东西是个很虚无缥缈的东西，对于我们财力有限的个人来说，基本上不会有什么实际的效果，或者应该说效果=o，也许你要说那些大网站，大门户，大论坛，广告多的吓死人，呵呵，那确实是多，但是对于他们来说的广告效果和我们眼中的广告效果又是不一样的，为什么这么说内，我们眼中的广告效果，最实际的就是给我们带来成交量，如果成交量不行的话，退而求其次，给我们带来一定的流量也算是对得起自己花的银子，然而那些大广告主投的广告大多数是卖产品，他们所要求的广告效果紧紧是展示，不需要给我的网站带来多少流量，卖出去几个产品什么的。

The therapeutic effect of HYJDT on glomerulosclerosis was observed by way of five aspects:(1) Effect on renal tissue morphology;(2) Effect on urine protein and blood creatinine and blood uria nitrogen ;(3) Effect on superoxide dismutase and malonydialdehyde in reual cortex;(4) Effect on matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-l (TIMP-1) in glomerulus;(5) Effect on MMP-9 mRNA and TIMP-lmRNA and type-1 plasminogen activator inhibitor (PAI-1) mRAN in glomerulus.

用药治疗12周取肾，HE、PAS染色，在光镜下观察肾组织形态学变化，并在透射电镜下观察肾小球超微结构的变化。用半自动生化分析仪检测肾皮质匀浆中SOD活力、MDA含量。用免疫组化技术检测肾小球中MMP-9、TIMP-1的蛋白表达，用原位杂交技术检测MMP-9mRNA、TIMP-lmRNA、PAI-lmRNA的基因表达。免疫组化和原位杂交结果，采用计算机图象分析系统测量各自的平均吸光度。所有的实验数据均用SAS6.12统计软件包进行统计学处理。

The impact of circulation on industrialization is to promote and coordinate the lubricating effect,the ripple effect,the inductive effect;while the impact of industrialization on circulation is mainly seen in the pulling effect,the buttressing effect and the infiltrating effect.

流通对工业化的影响可概括为促进与协调润滑效应、波及效应、诱导效应；工业化对流通的影响主要有拉动效应、支持效应和渗透效应。

One Wild Night It's a hot night, the natives are restless We're sweating by the light of the moon There's a voodoo mojo brewing at the go-go That could knock a witch off her broom We slither on in and shed our skin Make our way into the bump and the grind So I'm passing by, she gives me the eye So I stop to give her a light Chorus: One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild, one wild, one wild, one wild night Havin' as much fun as you can in your clothes Margarita had me feelin' alright It just might be that I found religion I've been on my knees for half of the night Then I'm rolling the bones with Jimmy 'no dice' Gonna take him for a couple weeks' pay Man, if you lose this roll I take your girlfriend home So I stopped But you're not gonna believe who comes walking out Chorus: One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild night (hey, c'est la vie) One wild night One wild night (life is for the living so You gotta live it up, come on let's go) One wild, one wild, one wild, one wild night Guitar solo Chorus: One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild night (hey, c'est la vie) One wild night One wild night (life if for the living so You gotta live it up, come on let's go) One wild, one wild, one wild, one.. One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone) For one wild night One wild night (hey,c'est la vie) One wild night One wild night All we've got is one wild night One wild night (4x) One wild night blinded by the moonlight One wild night 24 hours of midnight One wild night stepped into the twilight zone for One wild, one wild, one wild, one wild night.

一狂放的夜它是热的夜，当地人是不安定的由那里月亮的光冒汗是巫毒教mojo酿造在可能敲巫婆她的笤帚的去去的我们我们滑行并且流洒我们的皮肤做我们的方式成爆沸和研磨，因此我通过，她给我眼睛，因此我停下来给她轻的合唱：一狂放的夜一狂放的夜(24个小时午夜)一狂放的夜(我跨步入暮色区域和她左我的心脏以眩晕)一狂放，一狂放，一狂放，一狂放的夜 Havin同样多乐趣，象您在您的衣裳玛格丽塔酒能有我feelin它也许行是我发现了我是在我的膝盖为夜的一半的宗教我然后滚动骨头与吉米'没有模子' 去采取他为几星期的薪水人，如果您丢失我采取您的女朋友家的这卷，因此我停止了，但您不相信谁来走出去 Chorus： One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild night (hey， c'est la vie) One wild night One wild night (life is for the living so You gotta live it up， come on let's go) One wild， one wild， one wild， one wild night Guitar solo Chorus：一狂放的夜一狂放的夜(24个小时午夜)一狂放的夜(我跨步入暮色区域和她左我的心脏以眩晕)一狂放的夜(嘿， c'est la竞争)一狂放的夜一狂放的夜(生活，如果为生活，因此您得到居住它，进展我们是)一狂放，一个狂放，一个狂放，一个一狂放的夜一狂放的夜(24个小时午夜)一狂放的夜为一狂放夜一个狂放的夜(嘿， c'est la竞争)一狂放的夜一狂放的夜我们有的全部是一狂放的夜一狂放的夜(4x)一狂放的夜由月光蒙蔽了一狂放的夜24个小时午夜狂放的夜跨步入暮色区域为一狂放的一，一狂放，一狂放，一狂放的夜。

The Underwriter may terminate its commitment under the Underwriting Agreement at any time prior to the Latest Time for Termination if: there shall develop, occur, exist or come into effect: any new law or regulation or any change in existing laws or regulations or any change in the interpretation or application thereof by any court or other competent authority in Hong Kong or any other place in which any member of the Group conducts or carries on business; or any event or series of events resulting or likely to result in any change in local, national or international financial, political, military, industrial, economic or market conditions; or any change in the conditions of local, national or international securities markets (including but without limitation, the imposition of any moratorium, suspension or restriction on trading in securities generally on the Stock Exchange due to exceptional financial circumstances or otherwise); or any change or development involving a prospective change in Hong Kong taxation or exchange control which will or may affect the Group or a proportion of the existing Shareholders of the Company in their capacity as such, which, in the reasonable opinion of the Underwriter:(1) is or will or is likely to have a material adverse effect on the business or financial condition of the Group as a whole or the Rights Issue; or (2) has or will have or is likely to have a material adverse effect on the success of the Rights Issue or the level of Rights Shares taken up; or (3) makes it inadvisable or inexpedient for the Company to proceed with the Rights Issue; or there comes to the notice of the Underwriter: any matter or event showing any of the representations, warranties and undertakings made by PIL and the Company was, when given, untrue or misleading or as having been breached in any respect; or any breach by any of the other parties to the Underwriting Agreement of any of their respective obligations or undertakings under the Underwriting Agreement or under any of the PIL Letter of Undertaking, Option Undertakings or the Sub-underwriting Letter, then and in any such case the Underwriter may, upon giving notice to the Company and PIL, terminate the Underwriting Agreement with immediate effect.

倘发生下列事件，包销商可于最后终止时间前任何时间终止其根据包销协议之责任：以下情况将会出现、发生、存在或生效：香港或本集团任何成员公司经营或从事业务所在任何其他地方之法院或其他主管机关颁布任何新法律或法规，或更改现行法律或法规，或有关法律或法规之诠释或应用有变；或发生任何事件或连串事件导致或可能导致本地、国家或国际金融、政治、军事、工业、经济或市况有变；或本地、国家或国际证券市场之状况有变（包括但不限于因特殊金融状况或其他原因而暂时禁止、暂停或限制港交所之整体证券交易）；或香港税务或外汇管制有变或出现可能导致变动之事态发展，而将会或可能对本集团或本公司部份现有股东产生影响，而包销商合理认为上述情况：(1)会或将会或可能会对本集团整体之业务或财务状况或供股有重大不利影响；或(2)经已或将会或可能会对供股之成功或供股股份之认购数量有重大不利影响；或(3)令本公司进行供股变得不智或不宜时；或包销商得悉：任何事情或事件显示太平船务及本公司所作出之任何陈述、保证及承诺于作出时在任何方面属失实或构成误导或遭违反；或包销协议之任何其他订约方违反彼等各自根据包销协议或任何太平船务承诺函、购股权承诺或分包销函件之任何责任或承诺，而在此情况下，包销商可向本公司及太平船务发出通知即时终止包销协议。

When vitamin A effect having stout , lubricating skin , is short of, skin becomes dry , rough; Vitamin B has the fleck effect to extend a wrinkle , removes; Vitamin C may prevent the skin blood vessel from ageing , strengthen blood vessel elasticity , increase white skin, effect cutting down skin chromatosis; Decrease acne the and pigment come into being Wei Sheng Su E , have and resisting the effect old and feeble.

维生素A有强健、润滑皮肤的作用，缺乏时，皮肤变得干燥、粗糙；维生素B有舒展皱纹、消除斑点作用；维生素C可防止皮肤血管老化，增强血管弹性，增白皮肤，减少皮肤色素沉着的作用；维生素E可减少痤疮和色素生成，并有抗衰老作用。

Firstly, based on literature review and qualitative surveys, the modified risk factors that affecting the effect of hypertension management in the underdeveloped rural area were identified, the specific questionnaire and SIPHF were developed, and the first cross-sectional study was conducted. Secondly, the surveyed population were then divided into intervention group and control group, and periodic health education and following up were conducted to the intervention group for six months; Thirdly, the second cross-sectional study was conducted to evaluate the effect of intervention by qualitative and quantitative survey, the indicators include the knowledge awareness rate and the right belief rate for hypertension control, and the behavior prevalence related to hypertension intervention, the disease burden, the life quality and clinical outcomes resulted from hypertension, the dynamic tendency of blood pressure and the patients\' satisfaction. And the factors related to the effect of hypertension management was evaluated; Finally, SWOT analysis was conducted to evaluate the factors affecting the intervention process and effect, based on the results of qualitative and quantitative surveys on the factors related to effect of the intervention, and to provide evidence-based suggestions for SIPHF improvement and policy-making in the underdeveloped rural area in China.

首先，在文献综述和定性调查的基础上，确认影响欠发达地区农村高血压患者管理效果的因素，设计调查问卷和高血压管理方案，并开展第一次横断面调查；其次，将调查乡镇分为试验组和对照组，对试验组患者开展健康教育和定期的随访管理，对照组患者则按常规工作对待；第三，实施规范化管理6个月后，采用定量调查和定性访谈等方式评价管理效果，通过第二次横断面调查，对试验组与对照组干预前后的危险因素变化情况及干预效果进行评估，评估内容包括患者的知识、信念和行为等与高血压防治有关的危险因素指标，及其产生的疾病负担、生活质量、临床指标、血压水平变化趋势、干预组满意度等结果指标，并分析影响高血压干预效果的因素；最后，采用SWOT分析，根据定性和定量调查结果分析该类地区今后全面开展该项工作所面临的内部和外部环境因素，并为欠发达地区农村高血压规范化管理方案完善及政策制定提供循证建议。

The mechanism of additional effect is very complicated. Experiment indicated that the additional effect of shallow water-accumulating gob had significant impact on transient electromagnetic signals. And the additional effect could not be simply incorporated into disturbance. The cause of additional effect was analyzed theorically. The examples confirmed that a the application of additional effect in detection of water-accumulating gob can produce ideal results.

附加效应产生的机理很复杂，试验表明，浅层的积水采空区的附加效应对瞬变电磁信号的影响很大，不能将这种现象简单的归结为干扰，本文从理论上分析了附加效应产生的原因，实例证明将附加效应应用到积水采空区的探测中能取得理想的效果。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Add it into your food such as to yogurt, cottage cheese and such.

你可以把它加入到像酸奶、奶酪一类的食物中一起进食。

The man was rescued about three hours later when his partner arrived home, SAPA said.

SAPA说，这为男子在三个小时后妻子回家后被解救