查询词典 come into effect

The result makes clear, matrix scale, into discharge, wet the processing result that does the TN in comparing pair of sewage, TP all has extremely distinct effect, among them matrix (beehive is cindery ∶ vermiculite) scale is 1 ∶ 2, it is into discharge 28.8 L/d, wet dry than be 1 ∶ 5 when, best to the purify effect of TN, TP.

结果表明，基质比例、进水流量、湿干比对污水中TN、TP的处理效果均有极显著影响，其中基质比例为1∶2、进水流量为28.8 L/d、湿干比为1∶5时，对TN、TP的去除效果最好。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

After filtration of the air into a cold wind into the interior and motor in the 1.1 kilowatts, resulting from the effect of wind pressure 180Pa and 18,000 cubic meters per hour of air volume, the interior has the original foul smell of the hot air discharged through the doors and windows to achieve the cool, filter dust, ventilation, improve the efficiency of the effect of workers.

过滤后的空气变成冷风送入室内并在1.1千瓦电机的作用下形成180Pa的风压和每小时18000立方米的风量，将室内原有的污浊有异味的热空气通过门窗排出，从而达到降温、过滤风尘、通风换气、提高工人效率的效果。

To this kind of problem, a lot of parents can feel is intractable very, also feel be ashamed ages at opening, or does not ask these with " dot " comes prevaricate he, or collects the " that come," to hold the " that come in the arms to come from the hospital from ash-bin with " flicker

对于这一类新问题，很多家长会感到很是棘手，也感到羞于启齿，要么用"小孩子不要问这些"来搪塞他，要么用"从垃圾桶里捡来的"、"从医院里抱来的"来忽悠

It was the first Blackpool for both of us we've come to since our separation – I wasn't able to come to the festival year earlier as I was in the hospital and Victor did not come either.

这是在Blackpool对我们两个第一，我们是来，因为我们的分离-我不能来节一年前我在医院和维克多也没有前来。

But Chelsea wants me to come back, I come back and am very happy to come back.

可切尔西想让我回来，我就回来了，我也很高兴回来。

Come to one's rescuers 帮助 come true 实现 I know, but it's still a dream come true.

我知道 但这终究是一个梦想实现了。

Come to one's rescuers 帮助 come true 实现 I know, but it's still a dream that has come true.

我知 道但这终究是一个梦想实现了。

Ladies up in here tonight No fighting, no fighting We got the refugees up in here No fighting, no fighting Shakira, Shakira I never really knew that she could dance like this She makes a man wants to speak Spanish Como se llama, bonita, mi casa, su casa Shakira, Shakira Oh baby when you talk like that You make a woman go mad So be wise and keep on Reading the signs of my body And I'm on tonight You know my hips don't lie And I'm starting to feel it's right All the attraction, the tension Don't you see baby, this is perfection Hey Girl, I can see your body moving And it's driving me crazy And I didn't have the slightest idea Until I saw you dancing And when you walk up on the dance floor Nobody cannot ignore the way you move your body, girl And everything so unexpected - the way you right and left it So you can keep on taking it I never really knew that she could dance like this She makes a man want to speak Spanish Como se llama, bonita, mi casa, su casa Shakira, Shakira Oh baby when you talk like that You make a woman go mad So be wise and keep on Reading the signs of my body And I'm on tonight You know my hips don't lie And I am starting to feel you boy Come on lets go, real slow Don't you see baby asi es perfecto Oh I know I am on tonight my hips don't lie And I am starting to feel it's right All the attraction, the tension Don't you see baby, this is perfection Shakira, Shakira Oh boy, I can see your body moving Half animal, half man I don't, don't really know what I'm doing But you seem to have a plan My will and self restraint Have come to fail now, fail now See, I am doing what I can, but I can't so you know That's a bit too hard to explain Baila en la calle de noche Baila en la calle de d Baila en la calle de noche Baila en la calle de d I never really knew that she could dance like this She makes a man want to speak Spanish Como se llama, bonita, mi casa, su casa Shakira, Shakira Oh baby when you talk like that You know you got me hypnotized So be wise and keep on Reading the signs of my body Senorita, feel the conga, let me see you move like you come from Colombia Mira en Barranquilla se baila assay it! Mira en Barranquilla se baila as? Yeah She's so ***y every man's fantasy a refugee like me back with the Fugees from a 3rd world country I go back like when 'pac carried crates for Humpty Humpty I need a whole club dizzy Why the CIA wanna watch us?

淑女提高在这里今晚没有战斗，没有战斗我们向上争取难民在这里没有战斗，没有战斗 Shakira， Shakira 我从不真的知道她可以像这一样的跳舞她使一个男人想要说西班牙人哦宝贝当你谈话同类哪一你使一个女人去疯狂因此是明智的而且保存在读我的身体的告示而且我在今晚你知道我的臀部不是谎言而且我开始感觉它是权利所有的吸引，紧张做不你见到宝贝，这是完美嗨女孩，我能见到你的身体移动而且它是驾驶我发狂的而且我做不已经最微小的主意直到我锯子你舞蹈而且当你散步在跳舞上上面地板没有人不能够不理睬方法你动作你的身体，女孩而且每件事物如此料想不到-方法你左右方的它因此你能继续拿它我从不真的知道她可以像这一样的跳舞她使一个男人想要说西班牙人哦宝贝当你谈话同类哪一你使一个女人去疯狂因此是明智的而且保存在读我的身体的告示而且我在今晚你知道我的臀部不是谎言而且我开始感觉你男孩发生让去，真正的减慢做不你见到宝贝两头尖的中型雪茄烟哦我知道我是在今晚我的臀部做不谎言而且我开始感觉它是权利所有的吸引，紧张做不你见到宝贝，这是完美 Shakira， Shakira 哦男孩，我能见到你的身体移动一半动物，一半男人我做不，不真的知道什么我正在做但是你似乎有一个计划我的意志和自制能力有来到不及格现在，不及格现在见到，我正在做什么我能，但是我罐子不因此你知道那太是一一点点难的解释我从不真的知道她可以像这一样的跳舞她使一个男人想要说西班牙人 Shakira， Shakira 哦宝贝当你谈话同类哪一你认识你是得到我施催眠术因此是明智的而且保存在读我的身体的告示感觉康茄舞，让我见到你动作起来像你一样来从哥伦比亚她是如此每个男人的幻想像我一样的一个难民背面与来自第三个世界的国家我需要一全部俱乐部晕眩的为什么中央情报局需要对手表我们？

Ladies up Up in here tonight No fighting, no fighting We got the refugees up in here No fighting, no fighting Shakira, Shakira I never really knew that she could dance like this She makes a man wants to speak Spanish Como se llama, bonita, mi casa, su casa Shakira, Shakira Oh baby when you talk like that You make a woman go mad So be wise and keep on Reading the signs of my body And I'm on tonight You know my hips don't lie And I'm starting to feel it's right All the attraction, the tension Don't you see baby, this is perfection Hey Girl, I can see your body moving And it's driving me crazy And I didn't have the slightest idea Until I saw you dancing And when you walk up on the dance floor Nobody cannot ignore the way you move your body, girl And everything so unexpected - the way you right and left it So you can keep on taking it I never really knew that she could dance like this She makes a man want to speak Spanish Como se llama, bonita, mi casa, su casa Shakira, Shakira Oh baby when you talk like that You make a woman go mad So be wise and keep on Reading the signs of my body And I'm on tonight You know my hips don't lie And I am starting to feel you boy Come on lets go, real slow Don't you see baby asi es perfecto Oh I know I am on tonight my hips don't lie And I am starting to feel it's right All the attraction, the tension Don't you see baby, this is perfection Shakira, Shakira Oh boy, I can see your body moving Half animal, half man I don't, don't really know what I'm doing But you seem to have a plan My will and self restraint Have come to fail now, fail now See, I am doing what I can, but I can't so you know That's a bit too hard to explain Baila en la calle de noche Baila en la calle de día Baila en la calle de noche Baila en la calle de día I never really knew that she could dance like this She makes a man want to speak Spanish Como se llama, bonita, mi casa, su casa Shakira, Shakira Oh baby when you talk like that You know you got me hypnotized So be wise and keep on Reading the signs of my body Senorita, feel the conga, let me see you move like you come from Colombia Mira en Barranquilla se baila así, say it!

不会说谎女士们今夜在这里舞动起来没有是非没有争斗我们在这里舞动起来没有是非没有争斗我从未意识她能如此欢快的跳舞人们纷纷说起西班牙语你的名字是？美丽的我的家他的家哦宝贝当你这样说话的时候人们为你如此着迷所以用你的智慧继续细读我的舞蹈于是今夜我欢快的跳舞你知道我的舞蹈不会说谎于是我开始感觉到这一刻所有的吸引所有的不安你看见了吗这便是完美嗨女孩我能看见你舞动的身影于是我着迷于是我迷失直到看见你在跳舞当你舞动的脚步划过地面所有人都为你而注目女孩让人意想不到-忽左而忽右所以请继续跳吧我从未意识她能如此欢快的跳舞人们纷纷说起西班牙语你的名字是？美丽的我的家他的家哦宝贝当你这样说话的时候人们为你如此着迷所以用你的智慧继续细读我的舞蹈于是今夜我欢快的跳舞你知道我的舞蹈不会说谎于是我开始感觉到你的存在来吧让我们一起慢慢的舞动你看见了吗这便是完美哦我知道今夜我正欢快的跳舞我的舞蹈不会说谎于是我开始感觉到这一刻所有的吸引所有的不安你看见了吗这便是完美哦男孩我能看见你舞动的身影如梦如幻我不确定自己在做什么但是你却不同我的意志我的约束现在开始失去开始失去看我正做我能做的但却无法做到你知道这似乎太难解释他在夜晚的街道上跳舞他在白天的街道上跳舞他在夜晚的街道上跳舞他在白天的街道上跳舞我从未意识她能如此欢快的跳舞人们纷纷说起西班牙语你的名字是？

Article 144 The Government of the Hong Kong Special Administrative Region shall maintain the policy previously practised in Hong Kong in respect of subventions for non-governmental organizations in fields such as education, medicine and health, culture, art, recreation, sports, social welfare and social

第一百四十四条香港特别行政区政府保持原在香港实行的对教育、医疗卫生、文化、艺术、康乐、体育、社会福利、社会工作等方面的民间团体机构的资助政策。原在香港各资助机构任职的人员均可根据原有制度继续受聘。

Small wonder, then, that the Chinese spend more in the shop than any other group of foreign visitors do .

这样的小惊喜，使中国顾客比任何国家的人消费得更多。