查询词典 come into effect

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

To sb's notice bring/call to mind bring to a halt bring into action catch one's breath call down / lay a curse upon sb. cast a shadow over catch at shadows catch sb's eye catch fire catch / get a glimpse of catch sight of come to sb's knowledge come to sb's notice come to a point come /go into effect come into a fortune come into fashion come to a halt combination with commander in chief communication with commit a crime commit / make an error come into possession of sth. come / go into operation come to one's senses come to / arrive at a decision come to / arrive at an agreement with reach / make an agreement with come to sb.'s assistance complaint about/of competition with / against sb.

do a threat to do damage to do/try one's best 与----接触不平衡与-----保持友好与----一致对----负责违背诺言实施，使生效给某人带来羞辱使某人注意----想起使停止采取行动喘息诅咒某人蒙上阴影捕风捉影引人注目着火，烧着对----瞥一眼看见被某人知道引起某人的注意到紧要关头实施，生效继承一笔财产开始流行停止与------结合总司令与-----交流，与----通信犯罪犯错误占有某物实行，生效恢复理性，苏醒过来做出决定与-------达成一致与-------达成一致帮助某人对------抱怨与某人竞争对----有威胁损害-----尽力，努力

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

The Sailor Song 歌手:Toy-Box 专辑:Fantastic 歌词: So if we all come together, we know what to do We all come together, just to sing we love you And if we all come together, we know what to do We all come together just for you Racing all around the seven seas Chasing all the girls and making robberies 'Causing panic everywhere they go Party-hardy on Titanic Sailing, sailing, jumping off the railing Drinking, drinking,'till the ship is sinking Gambling, stealing, lots of sex-appealing Come, let us sing the sailor-song So if we all come together, we know what to do We all come together, just to sing we love you And if we all come together, we know what to do We all come together just for you Sailorman, you really turn me on Now the guys are gone, come let us get it on Girls like me are pretty hard to find So if you go, I'll kick your Heine Sailing, sailing, jumping off the railing Drinking, drinking,'till the ship is sinking Gambling, stealing, lots of sex-appealing Come, let us sing the sailor-song So if we all come together, we know what to do We all come together, just to sing we love you And if we all come together, we know what to do We all come together just for you Now, let's fight!

如此如果我们全部一起来，我们知道什么做我们所有一起来，唱我们爱您并且如果我们全部一起来，我们知道什么做我们全部一起来为您赛跑全部在七海附近追逐所有女孩和做盗案造成恐慌到处他们是党强壮在力大无比航行，航行，跳跃栏杆喝，喝，船下沉的'till 赌博，窃取，许多性呼吁来，让我们唱水手歌曲如此如果我们全部一起来，我们知道什么做我们所有一起来，唱我们爱您并且如果我们全部一起来，我们知道什么做我们全部一起来为您水手们，您真正地使我狂热现在人去，来让我们得到它女孩象我是相当困难发现如此如果您去，我会反撞力您的航行，航行，跳跃栏杆喝，喝，船下沉的赌博，窃取，许多性呼吁来，让我们唱水手歌曲如此如果我们全部一起来，我们知道什么做我们所有一起来，唱我们爱您并且如果我们全部一起来，我们知道什么做我们全部一起来为您现在，我们来战斗！

Cast a shadow over catch at shadows catch sb's eye catch fire catch / get a glimpse of catch sight of come to sb's knowledge come to sb's notice come to a point come /go into effect come into a fortune come into fashion come to a halt combination with commander in chief communication with commit a crime commit / make an error come into possession of sth.

与----接触不平衡与-----保持友好与----一致对----负责违背诺言实施，使生效给某人带来羞辱使某人注意----想起使停止采取行动喘息诅咒某人蒙上阴影捕风捉影引人注目着火，烧着对----瞥一眼看见被某人知道引起某人的注意到紧要关头实施，生效继承一笔财产开始流行停止与------结合总司令与-----交流，与----通信犯罪犯错误占有某物实行，生效恢复理性，苏醒过来做出决定与-------达成一致与-------达成一致帮助某人对------抱怨与某人竞争对----有威胁损害-----尽力，努力伤害某人为----贡献自己的力量知识改变命运，思路决定人生！

You Better Not Come Home U can't be trusted, boy you got busted, got me angry and disgusted, what's this, attitude no gratitude, what you doin' on the side boy, got me mad at you, but what I'm bout to do it tighten up, get rough, had enough, what's up, times are about to get real tough,- cause your covers got blown, now you out on your own - so you know you better not come back home Better not, better not, better not come home you better not come home Better not, better not, better not come home you better not come home It's really gettin\' hot tonight so u better not come home - uuuuh baby gotta treat me right so u better not come home - it's really gettin\' hot tonight so u better not come home - uuuuh baby gotta treat me right so u better not come home It\'s like hot in the kitchen why you act\'en like that, why you tripp\'en and flippen girl I see that you won\'t listen, it\'s like I\'m pippen on a underground mission, why you acten foul, girl you getten kinda loud,- with them accuasations, I can see you don\'t understand what i\'m facin\' the aggravation unjustified retaliation, girl you better wake up and tecognize the facts, ain\'t no need to act like that Now you playen out of bounds, better check your tags, I used to be a straight mack recognize homegirl I don\'t get down like that, way back in the days, had my street fame now I changed my game, better educated, straight up better dedicated, playen\' for real girl, never hesitated, so why you wanna go and leave your brotha alone, what you mean girl I better not come back home.

更好地为您不来主页ü不能信任，男孩你破获，让我感到愤怒和反感，这是什么，态度没有任何感激之情，你的乐队'的一方的男孩，让我疯了你，但是我比赛做它收紧，让粗糙，受够了，这是怎么回事，时间大约获得真正的困难，-造成您的涵盖了吹，现在你对你自己的-所以你知道你最好不要回来最好不要，最好不要，最好不要回家你最好不要回家最好不要，最好不要，最好不要回家你最好不要回家这真是gettin \ '热今晚所以ü最好不要回家- uuuuh婴儿对待我爱的权利所以ü最好不要回家-这真是gettin \ '热今晚所以ü最好不要回家- uuuuh婴儿对待我爱的权利所以ü最好不要回家这\ '就像在厨房里热为什么你的行为\ '恩这样，你为什么特里普\ '恩和flippen 女孩我看到你赢得\ '吨听，它\ '，就像我\ '米皮蓬在地下的使命，为什么你acten犯规，女孩你朋友太多还挺响亮，-与他们accuasations ，我可以看到你不\ '吨理解我\ '米facin \ '加剧不合理的报复，女孩醒来，更好地为您和tecognize的事实，艾因\ '吨没有必要采取这样的现在您playen出界，更好地检查您的标记，我曾经是一个连续麦克承认homegirl我不\ '吨开始这样，回程的日子，有我街名利现在我改变了我的游戏，更好的教育，更好的专用直线上升， playen \ '真正的女孩，从未犹豫过，为什么你想要去并留下您的brotha ，仅你的意思的女孩我还是没有回来。

Mosley Im glad youre my maker My Loyalty lies in your hands, youre my breath taker Your body, your kiss is in unknown demand So take command, go Timbo I be the same when it all goes up I be the same when it all goes down Not the first one, open it up I be the last one closin it out Dont know if Ill give you a shot yet Lil Mama Im peepin your style Do I think youre dope enough, yup One way of findin it out The way you came at me, boo Dont care, not afraid Im like Wild Really want it from head to toe Question if she gon let it out Anyway the hour glass go I dont worry anyhow Why dont we see where it go Lets figure it out When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after Dark Owww, Oooohhh Owww Come on SoShy I got a little secret for ya I never sleep when comes the night But everytime I smack my fingers I switch back into the light My moon belong to your sun Your fire is burning my mind Is it love or is it lust Something that I just cant describe Am I the one and only Cause youre the only one It felt so long and lonely Waiting for you to come Its lookin bright and early Im willing to close my eyes This is the unusual story Timbo and SoShy When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after Dark Heyy, Heyy, Heyy, Go Nelly!

莫斯利先生您好我很高兴youre进出口公司我的忠诚度掌握在你们手中，youre我的呼吸接受者你的身体，你的吻是在未知的需求因此，接受命令，去廷博俺是相同的时，所有的上涨俺是相同的时，一切都下降不是第一个，打开它俺是最后一次closin出来 Dont知道病患者给你一个镜头还进出口peepin律妈妈你的风格我认为youre涂料不够，烨一个方法是findin出来你的方式来我，嘘 Dont护理，不怕像野生进出口真的想从头到脚它问她是否坤把它放出但无论如何，沙漏去余请勿担心无论如何为什么dont我们看到它去可以让数字出来当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在上午的后当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在天黑后早晨 Owww，Oooohhh Owww 来吧SoShy 我得到了亚一个小秘密我从来不睡觉的时候来晚但是，每次我拍击我的手指我转回到光明我属于你的月亮太阳你的火是我心中燃烧是爱还是欲望一些我公正不能描述我是唯一的原因youre唯一的它认为这样漫长而孤独的等着你来它lookin一大早进出口愿意接近我的眼睛这是不寻常的故事廷博和SoShy 当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在上午的后当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在天黑后早晨日Heyy，日Heyy，日Heyy，直接耐莉！

Come up against come up smiling come up to come up with come upon come on come what may come what will come with come within Coming up!

职权范围走近；上来；长出，发芽；被提出；流行起来；进大学；进城；上升；抬头；呕吐；快！

The effect of treatment is negatively correlated with the course of disease, generally, the effect is much worse with the course of disease is much longer; The effect is positively correlated with the course of treatment, it is better if the course of treatment is longer.The group of more than one year is much excellent than the group of less one year. The ex-electroencephalogram before treatment is normal, on edge and a little abnormal, the effect is better. As regards CT or MR result, there is difference between the effective group and the ineffective group. The result of CT and MR is negative, the effect is better, and the effect to demyelination and leukoencephalopathy is most worst.However,for the tipe of much abnormality, the effect is the most worst.The effect is related to course of disease.The effect is better if the course is shorter.There is no difference in the tipe of disease the symptoms before episode, the frequency of episode, the inducing reasons, the age, sex and so on.

病程的长短与疗效存在负相关关系，病程越长疗效越差；疗程与疗效存在正相关关系，疗程越长疗效越好；疗前脑电图的异常程度与疗效存在负相关关系，正常、边缘及轻度异常者，疗效较好，重度异常者疗效最差；无效组与有效组在CT及MR检查结果上有差异，有阴性者疗效好，阳性者疗效差，脱髓鞘及脑白质病疗效最差的趋势；疗效与性别无相关性；无效组与有效组在发作类型、既往病史、辨证分型、发作先兆、疗前发作频率、诱因、发病年龄、就诊前所用药物方面无差异。

As a whole, this article investigates the investment effect problem from a new view of regional economics. It covers the economic, social and ecological effect of regional investment; it defines the concepts of investment effect and regional investment effect, deciphers the connotation, classification and influential factors of investment effect, and establishes a basic theory framework of investment effect research; constructs a fire-new regional investment effect evaluation model that could evaluate comprehensively the economic, social and ecological effect of regional investment, including evaluation index system and evaluation method, which supplies the gap of regional investment effect evaluation in academic community.

总体上看，本文从区域经济学这一新的视角研究投资效应问题，内容涵盖了区域投资的经济效应、社会效应和生态环境效应；界定了投资效应、区域投资效应的概念，解读投资效应的内涵、分类与影响因素，建立了投资效应研究的基本理论框架；构建一个对区域投资的经济效应、社会效应和生态环境效应进行综合评价，包括评价指标体系和评价方法在内的全新的区域投资效应评价模型，弥补了学术界在区域投资效应评价方面的空白。

No complex distressful things on your mind but only our love and care for each other.

无复杂痛苦的事情，对你的心，但只有我们的爱和照顾对方。

At first light, on his own initiative, Vick located the enemy emplacements andfired on them, using 15 rounds.

破晓时分，维克主动地去查找敌军的炮位并向它们开火，打了15发弹药。