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B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明：(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料；(2)在所试验到的MS、MS1/2（将MS的所有成分均减半）、WPM、DKW、B〓、SH等六种基本培养基中，MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基；(3)水曲柳的体细胞胚胎发生以直接发生为主，体细胞胚主要来自于从合子胚分离的完整子叶；(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响，诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2，5，10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0，2mg/L+TDZ 0.05mg/L；(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力，少数愈伤组织可以分化出体细胞胚；(6)愈伤组织的诱导也受培养基和激素配比的显著影响，最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2，5mg/L+TDZ0.05mg/L；(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力，此时种子尚未成熟，胚乳已呈固态，种胚的各个部分已分化完全，种胚体积占胚腔的大约2/3；(8)在各自综合的最适条件下，完整子叶的体细胞胚诱导率可达33.68%，下胚轴的愈伤组织诱导率可达90.7%。

Methods:43 patients including 10 with mitral stenosis associated aortic regurgitation, 12 with mixed mitral valve lesions and 21 with mixed mitral valve lesions and combined with aortic regurgitation were studied.MVA determinded by (1) two -dimensional echocardiography with a cross-sectional area in the short-axis view (2DE),(2) pressure half-time using formula of 220/PHT,(3) color Doppler flow imaging with a jet cross-sectional area in the short-axis view with planimetry,(4) CDFI with a color jet width at the mitral valve orifice in the apical four-chamber view and long-axis view, assuming an elliptic mitral orifice, compared with the orifice area of excised mitral valve during mitral valve replacement.

材料与方法∶分别用二维超声(2DE)、压差减半时间、彩色多普勒二尖瓣口血流截面积和椭圆形面积法测定43例风湿性心脏病二尖瓣狭窄拟行人工瓣置换术患者的MVA，其中合并主动脉瓣反流 10例，合并二尖瓣反流 12例， 21例，并与手术标本的实测面积相比较。

The relationship between the genetic deficiencies and hemorrhagic disorder were characterized. The homozygosity of the Thr359Met in the propositus with FⅦ: C activity 2% was related to severe clinical symptoms; The proband with double heterozygous lesions of Arg152Leu and one single nucleotide deletion at position 11487-9, combined with Arg304Trp in exon 8 with FⅦ activity 1% suffered severe clinical bleeding tendency. one proband with double heterozygous mutations (Agr304Trp and Arg304Gln) with 10% of FⅦ activities characterized asymptom. the heterozygous mutations (Thr359Met, Arg152Gln,-55C→T) with FⅦ: C 1%～5. 5% showed moderate or mild clinical symptoms.

其中8961 G＞T（Arg152Leu）和10966-8delC两种突变为国际首次报道，IFSla+5g＞a突变导致的异常剪接为国际首次报道。1个F7及F10基因联合缺陷家系，其中，F10基因28139 G＞T（Val384Phe）突变为国际首次报道；发现了1个由F7及组织因子(tissue factor，TF)两种基因联合缺陷导致的出血家系，其突变分别为F7基因启动子区-55C＞T杂合突变；TF 9363 C＞T（Arg131Trp）杂合多态性（频率2.63％）。6种F7基因突变发生在催化区；2种突变发生在裂解位点；1种发生在启动子区；1种突变发生在剪接位点，除一种缺失突变外，其余均为点突变；所有家系的基因突变都来自先证者的父亲和/或母亲。

Results The highest drug use frequency was biguanide. There was 50.9% in the oral antidiabetic drug combination. Leading the first 3 places on the list of drug combination were sulfonylureas in combination with biguanide, sulfonylureas in combination with α-Glycosidace inhibitor, biguanide in combination with α-Glycosidace inhibitor, while there was 16.1% in 3 kinds combination. When oral antidiabetic drugs were combined with other drugs, combination with antihypertensives drugs led the fist, while with antihypertensives drugs led the second.

结果 二甲双胍使用频率最高，口服降糖药联合用药的比例高达50.9%，居前3位的依次是磺脲类＋双胍类、磺脲类＋α-葡萄糖苷酶抑制剂和双胍类＋α-葡萄糖苷酶抑制剂，而3种以上口服降糖药联合用药的比例也达16.1%；口服降糖药与其他药物联合使用情况看，配合使用抗高血压药的频率最高，其次是降脂药。

Results 15 eyes of peripheral retinal dry hole and peripheral retinal degeneration were treated only by laser treatment and successful rate was 100%;10 eyes with local rhegmatogenous retinal detachment,were initially treated with herbal dehydrant,followed by laser treatment and successful rate was 80%;2 eyes failed due to the traction of vitrous were successfully treated with the aids of local pad;141 eyes with retinal detachment were treated by scleral buckling combined with krypton laser and success rate was 96.5%,5 eyes failed due to the traction of vitrous were successfully treated with the aids of vetroretional operation;27 eyes with complicated retinal detachment were treated by vetroretional operation,followed by intro????and post????

结果 视网膜周边干性裂孔和视网膜周边变性单纯用氪多波长激光治疗15眼，成功100%；局限性孔源性视网膜浅脱离10眼，先使用脱水剂、卧床休息、单眼加压包扎等措施，而后行激光治疗，成功率80%，2眼因玻璃体有牵引结合局部垫压术后成功；巩膜外扣带术后激光封孔141眼，成功率96.5%。5眼因玻璃体有牵引行玻璃体手术后眼内激光封闭裂孔成功；复杂性视网膜脱离经玻璃体切割手术，术中及术后激光封闭裂孔27眼，成功100%。

Results 15 eyes of peripheral retinal dry hole and peripheral retinal degeneration were treated only by laser treatment and successful rate was 100%;10 eyes with local rhegmatogenous retinal detachment,were initially treated with herbal dehydrant,followed by laser treatment and successful rate was 80%;2 eyes failed due to the traction of vitrous were successfully treated with the aids of local pad;141 eyes with retinal detachment were treated by scleral buckling combined with krypton laser and success rate was 96.5%,5 eyes failed due to the traction of vitrous were successfully treated with the aids of vetroretional operation;27 eyes with complicated retinal detachment were treated by vetroretional operation,followed by intro-and post-operation,and success rate 100%.

结果 视网膜周边干性裂孔和视网膜周边变性单纯用氪多波长激光治疗15眼，成功100%；局限性孔源性视网膜浅脱离10眼，先使用脱水剂、卧床休息、单眼加压包扎等措施，而后行激光治疗，成功率80%，2眼因玻璃体有牵引结合局部垫压术后成功；巩膜外扣带术后激光封孔141眼，成功率96.5%。5眼因玻璃体有牵引行玻璃体手术后眼内激光封闭裂孔成功；复杂性视网膜脱离经玻璃体切割手术，术中及术后激光封闭裂孔27眼，成功100%。

The survival rates of CEM cells cultured with cis-diamminedichicloroplatinum added 24 h later were higher than that cultured with hTERT ASODN and DDP added 24 h later. The survival rates of CEM cells cultured with DDP were similar with that cultured with hTERT SOND and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells treated with DDP or DDP combined with hTERT ASODN ro SODN at 48 h, displayed classic apoptotic changes. Apoptosis rates of CEM cells treated with DDP for 48 h after 24 h of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic cells of CEM cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively.

结果： hTERT ASODN作用于CEM细胞24 h再加入柔红霉素、长春新碱、足叶乙甙，对细胞生长的抑制分别与单用柔红霉素、长春新碱、足叶乙甙及hTERT正义寡核苷酸联合柔红霉素、长春新碱、足叶乙甙组相比，统计学上无显著差异(P＞0.05)。hTERT反义核酸作用于CEM细胞24 h加入顺铂，再共同作用48 h，CEM活细胞均数为2.318×108 cells/L，与单用顺铂组(3.250×108 cells/L)及hTERT正义核酸联用顺铂组(3.175×108 cells/L)相比，对细胞抑制明显增强(P＜0.05)。hTERT ASODN作用于CEM细胞24 h再加入顺铂作用48 h，细胞出现典型的凋亡形态学改变。hTERT ASODN与2.5 μmol/L顺铂联合作用于CEM细胞48 h的凋亡细胞百分率(19.47%)分别同SODN与顺铂联合作用组(6.97%)、单用顺铂作用组(6.02%)进行比较有显著差异(P＜0.01)。

In order to probe the relationships between wave equation offset domain common image gathers/angle domain common image gathers with migration velocity, we quantitatively derive the changes of ODCIGs and ADCIGs along with migration velocity on the assumption of uniform velocity medium with a horizontal reflector. We come to conclusion that ODCIGs are hyperbolas with half offset; on the contrary, they are ellipses. Combined with the results of depth focusing analysis, we find the apparent incident angles in ADCIGs are larger than the real incident angles when the migration velocity is larger than the true one and vice versa. With lower migration velocity, the residual moveout on ADCIGs is an ellipse with the tangent of apparent incident angle; otherwise, it's a hyperbola.

为了得到波动方程偏移距域共成像点道集和角度域共成像点道集与偏移速度的关系，在匀速单层水平反射情况下，对ODCIGs和ADCIGs随偏移速度的变化进行了定量的推导，得到偏移速度偏小时ODCIGs与半偏移距成双曲关系，反之成椭圆关系；结合深度聚焦分析，得到偏移速度偏大时ADCIGs中视入射角大于真入射角，反之，小于真入射角；在速度偏小时，ADCIGs上剩余时差与视入射角的正切值成椭圆关系，反之成双曲关系。

The paper investigates both block-type pilot linear minimum mean square error algorithm and IEEE802.16 OFDM channel estimation analysis. Simulation results confirm the block-type pilot structure is more suitable for IEEE802.16 system. An optimal pilot setting algorithm, based on space frequency domain, is explored and the pilot chart is presented. The proposed algorithm finds its application in MIMO-OFDM. Simulation results show the algorithm presents a good approximation to the perfect channel state information curve, with about 2dB difference in SNR. The simulation results also demonstrate the algorithm achieves fairly high stability in the environment of fast fading, performance of the proposed algorithm is better that of pilot training channel estimation.3. Taking into account the characteristics of IEEE802.16 OFDM system, OFDM system simulation platform is constructed in Matlab, exploiting simulink as a tool. OFDM modulation and demodulation simulation system are configured on LabVIEW platform. All the system signal processing is simulated, including defining system parameters, designing modulation model, and verifying all the proposed algorithms under different environments, such as white Gaussian noise, multi-path fading, with or without guard interval etc. Bit error performance is evaluated. The research provides valid theoretical basis for practical OFDM system performance evaluation.4. Taking advantage of software radio, the paper designs a hardware platform with both 256-IFFT/FFT and 512-IFFT/FFT OFDM schemes co-existing in one platform. You can predetermine one from the two schemes to carry out almost the same model function with different system performance and parameter setting.5. Referring to IEEE802.16 standard, the paper proposes a design method for generating signals and frames suitable for laboratory investigations implemented in laboratory environment.6. Based on 6701evm digital evaluation card, combined with analogy front-end, the paper designs a DSP software model to deal with baseband signal processing. An overall OFDM scheme, with modulation and demodulation function, is accomplished.

讨论分析了MIMO-OFDM中一种基于空频域的最优导频设置算法，给出了导频图案，通过仿真实验表明，该算法与理想的信道状态信息曲线非常接近，信噪比差距约在2dB左右，并且在快衰落条件下具有较好的稳定性，其性能要优于基于前导训练的信道估计方法。3、根据IEEE802.16OFDM系统特点，论文分别在Matlab中应用Simulink工具构建OFDM系统仿真平台、在LabVIEW平台上实现了OFDM调制解调仿真系统，模拟了整个系统的信号流程，进行了OFDM仿真系统参数的选择和调制模块的仿真设计、论证各算法性能，并根据各种不同的条件：例如高斯噪声、多径衰落、有无保护间隔等，对系统的误码特性进行了评估，为正确评价实际OFDM系统的性能提供了有效的理论依据。4、论文以软件无线电思想作为指导，提出了以256点IFFT／FFT为核心和以512点IFFT／FFT为核心的两种OFDM算法模式并存于同一个硬件平台、且可预选的方案，它们在参数选取和性能指标上有所差异，均实现了相似的模块和功能。5、论文参考IEEE 802.16无线网络标准的参数设置，针对本设计系统的应用环境和系统硬件的性能速率，提出了一种应用于实验室环境的信号结构、帧格式等参数设计。6、论文基于TI公司的6701evm数字评估板卡，结合模拟前端搭建数字中频平台，设计了基带处理的DSP软件模块并进行系统调试，基本实现了一套完整的OFDM调制解调方案。

I'll describe a shot from the Exterior Brookfield sequence of an exterior of a row apartment on fire with an explosion blasting out the third story windows. The shot was composited on an Inferno by Bob Wiatr. We started with a live plate of the street scene. Our first task was to remove a foreground chainlink fence and camera crew. We recreated the fg matching the flashing ambulance lights. To create a snowy, misty look we used a number of logic ops of the bg with a mist plate which was basically the bg heavily blurred and multiplied back onto itself, graduating to create a sense of dimensionality. Glows were added around lights and flames using Sapphire plug-ins and light beams were added to streetlights. Four or five layers of 3d particle snow created in Maya were combined with practical elements varying in size and focus depending on distance from the camera.A miniature explosion element was incorporated into the comp, also with added glows, interactivity with the building, and rotoscoping of foreground objects. The entire comp was then time warped for editorial matching with the subsequent cut.

我可以描述一个Exterior Bookfield的几个镜头，那是一排的公寓着火，在三楼窗户引起爆炸，这个镜头是Bob Wiatr用Inferno合成的，我们用一个现场的街道镜头开始，我们的第一个任务是删除一个在前景中的链条状的篱笆和摄像人员，我们重新制作了前景以期与前景中的闪烁的救护车灯匹配，为了制作一个下雪的雾蒙蒙的效果，我们用了大量的logic ops，用了一个雾层，这个层基本上就是把背景尽量模糊然后再把它重新加到背景上面，渐变式的制作出一个层次感，自发光用Sapphire插件加在灯光和火焰的周围，而光线被加到路灯上，在maya中制作的四到五层的三维的粒子雪用来和不同大小的真实的素材混合在一起，焦距则依赖镜头的景深，同时在楼房爆炸的地方加入了一个缩小了的爆炸素材，以及爆炸的辉光效果，和重新放置的前景物体，整个的镜头接着匹配时间用来做最后的剪辑制作。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。