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The results indicated that the content of cytosolic CaM in cells treated with exogenous Ca2+ has increased indistinctively, while fluorescence intensity in cells treated with TFP decreased. So we believed that exogenous Ca2+ has little effect on the expression of CaM. High concentration of TFP can enter yeast cells and combine to CaM to make it inactive, which is the reason that TFP restrain the growth of yeast cells. 5mmol/L EGTA could completely arrest the cell proliferation of MFP7 after 28h, when the fluorescence intensity in cells wasobviously increased with flow cytometry and LSCM.
被较高浓度TFP处理过的MFP7胞内荧光强度则相对较弱,推测是因为TFP进入胞内与CaM结合从而使其失活,这也是高浓度TFP抑制酵母细胞增殖的原因。5mmol/L EGTA处理28h左右后,酵母细胞的生长被抑制在G1晚期,此时通过细胞流式法和在激光扫描共聚焦显微镜下观察到胞内荧光强度显著高于对照,说明CaM的表达水平在G1后期开始上升;回加10mmol/L Ca~(2+)处理24h后,细胞开始恢复生长,细胞流式法测定胞内荧光强度有所下降,表明多数细胞完成G1/S转换,进入生长对数期。
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the digitized mapping technique is to combine the field data collection system with the computer assisted mapping system in surveys by computer technology.it aims to realize the information collected and processed digitally and automaticaity.the digitized mapping technique can cut short the working time,lighten the labor intensity and enhance the precision of the productions.the system consists of three parts,such as data input,data processing and data output.the survey-record digitized mapping technique is widely used in the digitized mapping working pattern.for its superiority over traditional plane-table mapping in accuracy and efficiency,the large scale digital mapping is becoming more and more popular.compared with traditional analogue mapping,digital mapping has more quality control pivotai points,and its contents and methods are more complex.with the appearance of new technology gps ,the coordinate of different levels controlling points may be surveyed in high precision and it has been applied widely in topographic survey.the operation process of gps electronic tachometer is introduced and its application in topographic survey is br
计算机应用论文,计算机论文, rtk ,全站仪,数字测图,地形测量, gps论文摘要:数字测图是在测量工作中利用电子计算机技术将野外数据采集系统与内业机助制图系统相结合,其目标是实现信息采集处理的数字化、自动化、信息化。数字测图可以缩短作业时间,减轻劳动强度,提高成果精度。数字测图系统主要由数据输入、数据处理和数据输出3部分组成,数字测图作业模式中测记式数字测图应用最为广泛。大比例尺数字测图正以其测图精度高,成图速度快等优势逐步的取代传统的,以平板仪为主的模拟测图。与传统的模拟测图相比,数字测图的质量控制关键点更多、内容与方法更为复杂。gps 新技术的出现,可以高精度并快速地测定各级控制点的坐标,在地形测量中已得到广泛地应用。本文介绍了gps 配合全站仪的作业流程,简要阐明了其在地形测量中的应用。在利用实测数据成图的过程中,解决一些常见的问题,并给出解决的办法及依据,同时给出一些有益的结论,以适应实际使用的需要。
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This text human life state problem root visual field in human life meaning the inside, Increase actual in the demand of contemporary human life state, with the human life"s important function that China with the west already the cognition of the some human life state is a foundation to shine on, pass to analysis human life state innate character with study, announce to public the human life state to path, combine as to it"s the exaltation proceeds studying of first step.
本文将人生境界问题植根于人生意义的视野中,立足于引导和提升当代人人生境界立场,以中国和西方已有的人生境界的理论认识为基础和参照,通过对人生境界本质的分析和研究,揭示了人生境界对人生的重要作用,并对其提高的途径进行了初步的探讨。全文共分为三大部分:第一部分主要阐述人生境界的本质。
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Pass to count to Wisterch parameter , and the steel wire is different from the molding tool get in touch with the length, and settle the path the research taking the length work the stabber angle to get under differently pull to stir the term, mold core adoption of best severally why size, combine the best work stabber of research the Cape, and settle the path to take the length nextly steel wire is in the molding tool of have fever to measure, air inside dispel-heat calories, winding dispel-heat on the coil-canister , and its molding tool dispel-heat calories of, and cause the steel wire the temperature difference pass in and outing the molding tool to influence steel wire machine function, under the experiment inside pull to stir the term according to the same race different work stabber Cape half of adoption the Cape(7 degree,10 degree,12 degrees, the best mold Cape, experience mold Cape) settle the path to take the length(2-3 mm,3-4 mm,4-5 mms, theories length, experience length) to proceed to experiment, and get the conclusion, work stabber the Cape then is more big the steel wire get in touch with the length with
通过对Wisterch参数,钢丝与模具接触长度、定径带长度、工作锥角度的研究得到了在不同拉拨条件下模芯采用的最佳几何尺寸,并研究最佳工作锥角、定径带长度下钢丝在模具中的发热量、空气中散热量、卷筒上的散热量及其模具散热量的不同,导致钢丝进出模具的温度差别对钢丝机械性能的影响,在实验中根据同种拉拨条件下采用不同工作锥角半角、定径带长度进行试验,得出结论,工作锥角越大则钢丝与模具接触长度越短,钢丝变形越剧烈,在模具中发热量就越高,导致钢丝出模温度高,使钢丝强度、硬度升高,弯曲、扭转次数降低,并且降低模具使用寿命。在其它条件相同的情况下,定径带越长则钢丝与定径带简壁磨擦增大,使钢丝发热量加大,从而使钢丝强度、硬度略升高,弯曲扭转次数略降低但定径带的增大减小了钢丝变形产生的弹性后效使钢丝出模直径比较稳定。
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Secondly, On the basic of existing research about mode classification of E-U-R cooperation, combine high school in Xinjiang in the aspects of producing to learn to grind to cooperate of actual, high school in Xinjiang in the research result conversion, product and technical developments and produce to learn to grind the cooperation to induce appliedly serves to consult with the mode, technique to turn to let with technique for the technique the mode, cooperation development mode, set up the entity mode, school to do the industry mode, the university science and technology park totally of the mode six greatest modes, detailed analyzed the influence factor, merit and shortcoming of the manifestation, characteristic, implement of a mode and open the exhibition circumstance in Xinjiang, return for a mode chose the typical model with meticulous care of case example.
其次,在参考现有研究对产学研合作模式分类的基础上,结合新疆高校在产学研合作方面的实际,把新疆高校在科研成果转化、产品和技术的开发和应用方面的产学研合作归纳为技术服务和技术咨询模式、技术转让模式、合作开发模式、共建实体模式、校办产业模式、大学科技园模式六大模式,详细分析了每种模式的表现形式、特征、实施的影响因素、优缺点以及在新疆的开展情况,还为每种模式精心选择了典型的案例。
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Some methods aiming at the two problems have been researched. First, the optimal number of ATE site can be calculated based on a cost model of DAT-ATE. Second, the problem of test schedule which is equitant with two-dimensional Bin-Packing problem is presented. Then a TCG denotation satisfied with T-admissible rule is more convenient for combinational optimization. With Simulated annealing algorithm, better test scheduling results can be achieved; but there is no great optimization space because the efficiency of scheduling is greater than 90%. Third, A novel test compression method——Multi-capture testing is proposed to compress the stimuli. And the ATPG results show that MC achieves high compression ratio which is greater than 90% in some large-scale circuits. MISR, widely used in LBIST, is employed to compress the responses. The aliasing analysis demonstrates that the fault coverage reduction is little due to the existence of aliasing. Fourth, an improved TIC (called S-TIC) aiming at structured test is proposed based on ARM's TIC. A MC scheduling algorithm is proposed to compute the SoC test time and combine MC vectors to SoC test vectors. The scheduling results shows that test time is greatly reduced when using the proper scheduling priority.
首先,给出了低成本ATE的成本模型,根据此模型得到最低测试成本时系统级测试调度的基本参数——测试Site数;其次,本文将DAT-Scan方式的SoC测试调度等价为两维BP问题,为有效地求解该问题,将该BP问题表示为TCG图并通过模拟退火算法解决其求解过于复杂的问题,调度的结果表明该方法在测试时间上有一定改善,并指出DAT-Scan测试调度效率已经大于90%,进一步优化的空间较小;第三,本文尝试通过测试压缩来解决激励所需的测试带宽,提出了Multi-capture结构并解决了MC测试过程中的&矢量&退化问题,MC测试的ATPG结果表明其测试压缩率很高(大电路接近90%);第四,在激励获得很高压缩比的情况下,测试响应也需压缩,本文采用LBIST中常用的MISR作为MC测试的响应压缩电路,理论分析和实验结果都证明了MC测试的别名对故障覆盖率影响较小(小于2%),并对两类别名的成因做了具体分析;第五,在MC测试及其响应压缩的基础上,本文改进了ARM公司的测试控制器TIC使之适合于MC测试,为了解决MC测试矢量合成问题,本文抽取了MC测试模型,通过固定优先级的MC测试调度模拟将MC矢量合成为ATE矢量,并模拟出了总的MC测试时间。
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The main contributions of this research lie in that, Firstly, with reference to major research in this field, the study does not confine itself to the common methods used. With the study of the foundermental financial institutions as a starting analysis point, it helps to broaden the view of international academic research on operational risks, and deepen the understanding of the intensions, characteristics and attributes of commercial banking operational risks. Secondly, studying operational risks on the basis of the unique economic and institutional conditions of China and utilizing the concept of operational risks to redissect the risk structure of SOBs contributes to the comprehensive understanding of the root risk structure of China's financial risks and the practical difficulties encountered in the governance of SOBs. It also helps to implore how to combine the international standard rules with the economic development and the financial system reform.
本研究的主要意义在于:第一,博采众长,不拘泥于现有银行风险研究方法,从对基本金融制度的探讨展开分析,有助于拓宽学术界研究操作风险的视角,同时也深化了对商业银行操作风险的内涵、特征和属性的认识;第二,立足中国国情研究操作风险,利用操作风险概念重新解析国有商业银行风险结构,有助于更全面地理解中国金融风险的深层结构和中国国有银行治理的真正困难,为探索如何将国际通行规则与中国经济发展和金融体制改革有机结合开辟了新思路;第三,在理论研究和实证研究的基础上,为商业银行操作风险管理和监管部门监管提供对策,对于操作风险管理和监管的现实操作具有借鉴意义。
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The fifth chapter combine the contemporary international social research on the principle of use of force with the international anti-terrorism, which is the issue that this thesis needs to solve, with the international terrorism deluging and under the gradual arduous international condition to fight against the terrorism, this chapter mainly does some research on the manners and methods to battle against the terrorism as well as some issues relating with anti-terrorism in the international law.
然而冷战的结束并没有使我们生活的地球变成一个没有争端也没有冲突的完全和平的星球,各种国际争端和武装冲突不断的提醒我们在通向和平的道路上荆棘密布。尽管完全的和平还无法企及,但维持国际和平与安全无疑是当今国际社会各国共同追寻的目标,禁止非法使用武力,和平解决国际争端是实现这一目标的国际法保障。
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Why not combine live action with animation together with his friend ub iwerks, the two youngsters began production of the alice and cartoonland films in which a live girl romps with her animated friends.
于是他和朋友乌伯伊瓦克斯一起开始制作第一部电影《爱丽丝在卡通国》:讲述了一个小女孩和她的卡通朋友的经历。
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Secondly, two stage method is adopted in the selection of development strategy. In the first stage, combine the development strategy group with the result of SWOT to select the proper strategy. In the second stage, use AHP and QSPM to evaluate the spare development strategy project, then present the final combination strategy of "BOM to continue existing strategy, BOB and Twist web with contractile strategy; No-woof blanket and Asbestos blanket with giving up strategy".
采用两阶段法进行发展战略的选择,第一阶段结合战略群模型和SWOT分析结果对公司各产品可能采取的发展战略进行初次选择;第二阶段利用AHP分析结果和QSPM方法评价第一阶段备选的发展战略,提出了&BOM毛毯采取集中发展现有业务战略,BOB毛毯和螺旋干网进行战略收缩,无纬毛毯和石棉瓦毯采取放弃战略&的战略组合方案。
- 推荐网络例句
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This one mode pays close attention to network credence foundation of the businessman very much.
这一模式非常关注商人的网络信用基础。
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Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.
扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。
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There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.
双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。