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EST similarity analysis was finished by comparing sequences with BLASTx searches in NCBI. The results showed that they are proteins involved in biological processes such as energy metabolism and RNA molecular level regulation. Some of them are enzymes and structural proteins in the cell. This study provides useful information for future studies on molecular mechanism of filament coloring and for breeding new silkworm variety that spin color cocoons.

通过BLAST比对和对所得表达序列标签的同源分析表明,这些基因主要涉及能量代谢因子、RNA分子水平相关调节因子、酶类、结构蛋白等,由此初步探明了参与黄血基因协同作用的相关基因表达蛋白的种类和数量,可为进一步研究蚕茧着色的分子机制及通过遗传选择培育天然有色茧品种提供基础信息。

Methods Three-color fluorescent flow cytometry was used to analyze the percentage of peripheral blood T cell subsets in the young and elderly groups.

方法采用三色免疫荧光标记流式细胞术分析年轻组(21~59岁)与老年组(≥60岁)外周血T淋巴细胞亚群水平。

The experimental group has various changes in the different density of potentill anserine extract. The small rat pulmonary tissue surface ofⅠgroup can see sporadic petechia of different degree; pulmonary alveolus cavity can see sporadic erythrocyte under the microscope. The small rat pulmonary tissue surface of Ⅱ group is no obvious abnormality by naked eye; pulmonary alveolus cavity can see sporadic erythrocyte and thin powder color liquid under the microscope. Two groups all have the capillary vessel of alveolar wall dilation and the endothelial cell hyperplasia and hypertrophy in different degree.

实验组不同浓度的蕨麻提取物对小鼠肺组织的影响不同,Ⅰ组小鼠肺组织表面肉眼见不同程度的散在点状出血、显微镜下肺泡腔内见散在红细胞;Ⅱ组小鼠肺组织表面肉眼无明显异常,显微镜下肺泡腔内见散在红细胞及淡伊红色液体,两组均见肺泡壁毛细血管扩张充血及毛细血管内皮细胞呈不同程度的增生肥。

The experimental group has various changes in the different density of potentill anserine extract. The small rat pulmonary tissue surface ofⅠgroup can see sporadic petechia of different degree; pulmonary alveolus cavity can see sporadic erythrocyte under the microscope. The small rat pulmonary tissue surface of Ⅱ group is no obvious abnormality by naked eye; pulmonary alveolus cavity can see sporadic erythrocyte and thin powder color liquid under the microscope. Two groups all have the capillary vessel of alveolar wall dilation and the endothelial cell hyperplasia and hypertrophy in different degree.

实验组不同浓度的蕨麻提取物对小鼠肺组织的影响不同,Ⅰ组小鼠肺组织表面肉眼见不同程度的散在点状出血、显微镜下肺泡腔内见散在红细胞;Ⅱ组小鼠肺组织表面肉眼无明显异常,显微镜下肺泡腔内见散在红细胞及淡伊红色液体,两组均见肺泡壁毛细血管扩张充血及毛细血管内皮细胞呈不同程度的增生肥大。

The object of this study is to evaluate the immunoregulation and anti-tumor activities of Arnebia root polysaccharide in vitro. The ARPS was obtained by hot-water extraction, alcohol precipitation, de-protein, de-color, and dialysis etc. from Arnebia root and was administered on mouse spleen lymphocyte and tumor cell with 24 μgmL^(-1), 121 μgmL^(-1), 364 μgmL^(-1), respectively.

为研究紫草多糖在体外对小鼠脾淋巴细胞的免疫调节活性及对肿瘤细胞的细胞毒作用,本实验采用水提醇沉法,经脱蛋白、脱色、透析等方法提取紫草多糖,MTT法检测紫草多糖对淋巴细胞增殖及对HepG2、SPC-A-1肿瘤细胞的杀伤作用。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果:1合成的CS-PAA磁性聚合物微球呈球形,大小均一,TEM测定其干态下粒径为80nm左右,磁化曲线证实具有超顺磁性,具有一定的PH敏感性,固载表柔比星后,水溶液性状稳定,无沉淀物,DLS测定直径约200nm左右,测定载药率为15%,较文献报道高,包封率为20%。2肉眼观察试验组大鼠膀胱表面呈褐色,可见大量的Fe粒子聚集,普鲁士兰染色法显示,试验组大鼠膀胱壁内有大量的Fe粒子,分布至膀胱壁全层,与对照组大鼠相比,试验组大鼠的肝、脾内的Fe粒子聚集量明显降低;HPLC测定结果与Fe染色相同;高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高,其他组大鼠血生化指标没有明显变化。3MTT法发现,高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组,FCM发现试验药物组可引起明显的肿瘤细胞凋亡,试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C,试验组细胞Bcl-2蛋白减少,Bax蛋白变化不明显,Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P<0.01),其中高剂量组的抑制率最高。

In PsNPV infected treatment, the color of the gland was purple-black dyed with eosin and the cells were distortedly squeezed together. The tracheae nearby showed grievous pathological changes and a lot of white granules deposited around it. The pathological changes in PsGV infected treatment showed another pattern. The gland body was relative small with smaller individual cells, most of which were red dyed with eosin. The cell limits were obscure.In coinfected treatment,there were few gland cells colorated by esoin.

结果表明,不同感染组粘虫前胸腺腺体都有不同程度的组织病变,PsNPV感染组在感染晚期与前胸腺相连的气管严重病变,出现大量白色颗粒状物累积,被伊红染成紫黑色,腺体细胞被挤压变形;PsGV感染组的前胸腺腺体变小,单个细胞也小,细胞界限不十分明显;两种病毒混合感染组的腺体细胞小,能被伊红染色的细胞极少。

ERK1/2 immunohistochemistry positive staining coloration in cornu dorsale medullae spinalis of rats was Buffy color.Positive cells distributed in gray nucleus that nerve cell concentrated,and deep staining region localized at the nucelus of neurocyte.

针刺前后脊髓未见明显形态学改变。2、光镜下观察,大鼠脊髓背角ERK1/2免疫组化反应显色呈棕黄色,阳性细胞分布于脊髓灰质神经元集中的部位,针刺后深染部位向胞核集中。

Since this is not a mixed phosphor Cathode Ray Tube, you do not have to worry about overload in Red and Blue on a TWiGi or cell box, colorimetry is the same, and color fringing due to lens limitations is as before.

因为这不是一个混合的荧光粉阴极射线管,你不必担心在TWiGi中红色和蓝色的过载,因为镜头的限度为题,色度和色彩散射都是一样的。

11Th, accumulator cell when wiring must pay attention to the electric wire color, the homochromy docking, may not the unwanted response.

蓄电池在接线时须注意电线颜色,同色相接,不可错接。

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