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At the same time, intact shuttle-plasmid pZ189 was also introduced into the host cell which was used for detection of nontargeted mutation frequency. After replicating in cells, progeny plasmid DNA was isolated and transformed into E coli. MBM7070.In the presence of ampicillin, X-gal and IPTG, pZ189-transformed E. Coli MBM7070 forms blue colony if the plasmid contains an active SupF suppresser tRNA gene, and white or light blue colony if the SupF tRNA gene is inactive.

但是由于检测非定标性突变需同时引入穿梭质粒pZ189在细胞中复制，并回收质粒DNA，通过转化宿主菌E.coliMBM7070，在含x-gal和IPTG的氨苄指示板上培养，如果用于检测突变的靶基因SupF tRNA基因发生突变，则菌落色泽为白色或淡蓝色，而正常者为兰色。

Using file materials and the insiders oral account materials mainly, this text carried on deep case study to administrative institute of Japanese colony and captive in Nanjing, which including a great deal of respects, such as inner management system and management policy performance, etc. It have certain reference value to understand the whole condition of managing Japanese colony and captive in Chinese theater of operations.

本文主要运用档案资料以及知情人的口述资料，对当时设在国民政府首都南京的日侨日俘管理所的成立、内部管理体系以及管理政策实施情况等诸多方面进行了深入的个案研究，这对了解整个中国战区日本侨俘的管理有一定的参考价值。

The colony positive rate exam which after the trisaccharide iron urea indol culture medium is 28.91%,higher than the exam the colony positive rate using the trisaccharide iron culture medium (7.67%).

经三糖铁尿素靛基质培养基筛检出的菌落阳性率为28.91%，比三糖铁培养基筛检出的菌落阳性率（7.67%）高。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

The effects of heat shock on the conidial germination, colony growth, colony and mycelium morphology of Setosphaeria turcica were analyzed in this paper.

本文通过对玉米大斑病菌进行热激处理，观测热激对病菌孢子萌发、菌丝生长速度、菌落形态、菌丝形态等方面的作用。

The article expounds several main qustions,viewpoints,thoughts existing in the current education labour union colony from the following four aspects:change concept,maintain inculcator legal rights and interests and attend their body and mind health,discuss management mode and capital source of the colony work,perfect evaluation system and limit "minority pepole"phenomena.

从转变观念，维护教工合法权益、关注教工身心健康，群体工作管理模式、资金来源，完善评价体系、遏制&少数人体育&现象四个方面，阐述了当前教育工会群体工作存在的主要问题和观点。

Based on the finite element analysis of single pearlitic colony with different interlamellar spacings,the reason of the interlamellar spacing is investigated,which plays an important role in the mechanics behavior of single pearlitic colony .

作者从对具有不同片层间距的珠光体团的有限元分析结果出发，定性分析了层间距对其力学性能及疲劳寿命的影响机制。

In the nest of a mature monogynous colony, are 50,000-240,000 Red Imported Fire Ants. In a polygynous colony, the number of individuals doubles. If there are 300 monogynous colonies per hectare, the total number of fire ants in an area of 2 hectares will far overtake Taiwan's human population.

一个成熟的「单蚁后」蚁巢中至少有5-24万只入侵红火蚁个体，而成熟的「多蚁后」蚁巢中则最少有10-50万只，若以每公顷有300个「单蚁后」蚁丘的最保守方式估算，2公顷内的火蚁数就远超过居住在台湾的总人口数。

I n the nest of a mature monogynous colony, are 50,000-240,000 Red Imported Fire Ants. In a polygynous colony, the number of individuals doubles. If there are 300 monogynous colonies per hectare, the total number of fire ants in an area of 2 hectares will far overtake Taiwan's human population.

一个成熟的「单蚁后」蚁巢中至少有5-24万只入侵红火蚁个体，而成熟的「多蚁后」蚁巢中则最少有10-50万只，若以每公顷有300个「单蚁后」蚁丘的最保守方式估算，2公顷内的火蚁数就远超过居住在台湾的总人口数。

In the scorch stage, average yield of withered grass of second and third year growth prematurity type of Phleum pratense L. is 10672.5 kg/hm2, by cradling one time per year, average yield of wind hay grass is 7575 kg/hm2, the plant average height of selective colony is 160.0cm, the plant height of colony is rather equality. In the course of general nutrition composition analysis, discover that nutrition value of late-maturity type is higher than nutrition value ofprematurity type, coarse fibre and coarse protein and coarse fat of late-maturity type are 10.7%, 3.58%, 24.82% respectively, coarse fibre and coarse protein and coarse fat of prematurity type are 9.84%, 2.61%, 27.59% respectively, dry matter content of prematurity type is 92.63%, dry matter content of late-maturity type is 92.22%.

在枯黄期，第二、第三年生的早熟类猫尾草一年收割一次，枯草平均产量为10672.5 kg/hm~2，风干草平均产量为7575 kg/hm~2，选育出的群体平均高度为160.0cm，群体内植株株高较均一；在常规营养成分的分析中发现晚熟类的营养价值稍高于早熟类，晚熟类的粗蛋白是10.7％，粗脂肪是3.58％，粗纤维是24.82％，早熟类的粗蛋白是9.84％，粗脂肪是2.61％，粗纤维是27.59％，早熟类干物质含量92.63％，晚熟类干物质含量92.22％。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了