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Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

Methods: A total of 56 pedigreed members were collected. 62 patients with type 2 diabetes mellitus and 82 healthy were collected as controls. HNF-1β gene exon2 from all subjects were examined by polymerase chain reaction-single strand conformation polymorphism.

运用聚合酶链反应-单链构象多态性技术检测2型糖尿病患者62例，健康献血者82例，11个糖尿病家系中56例糖尿病肾病患者的HNF-1β基因exon2片段。

If Baidu is right of your website friendly degree not tall, how to accelerate the odds that is collected, through research, it is OK that I discover a website page to collect a few nets to pick greatly shorten the time that your website is collected, spent 9 hours re-edit today then cast the net pick code, each coping that had put this station page now!

假如百度对你的网站的友好度不高，怎样加快被收录的几率呢，经过研究，我发现把网站页面收藏到一些网摘可以大大的缩短你网站被收录的时间，于是今天就花了一两个小时重新编辑了下网摘代码，现在已经放到本站页面的各个顶部了！

In this study, six campaigns around Anping tide gauge, Tainan, were successfully performed and the collected GPS buoy data were processed with four types of precise ephemeris provided by IGS, including final product, rapid product, ultra-rapid product and ultra-rapid product with the use of PPP technique. Comparing the PPP results with DGPS, the differences reach 3~5 cm in the horizontal and 10 cm in the vertical with final product; 6~8 cm in the horizontal and 15 cm in the vertical with rapid product; 15~20 cm in the horizontal and 30~40 cm in the vertical with ultra-rapid product; 2~3 m in the horizontal and 3~4 m in the vertical with ultra-rapid product. In addition, the collected data were also processed by DGPS techniques using different reference stations to analyze the effect of various baselines. The results show that accuracy degrades when the baselines increase.

本研究在台南安平潮位站旁进行6次GPS浮标施测，首先利用与GPS浮标距离不同之GPS参考主站来进行差分定位，分析基线距离对GPS浮标定位成果的影响，由实验结果可得出基线越长则定位准确度越低；再以IGS提供之最终产品、快速产品、超快速产品(Ultra-Rapid product)之观测部分observed half、超快速产品(Ultra-Rapid product)之预估部分四种不同发布延迟时间的精密星历与精密时表改正资料对GPS浮标进行精密单点定位解算，与传统差分相对定位方法定位结果进行比较后，得出使用最终产品之平面方向均方根误差(Root Mean Square Error， RMSE)可达3~5公分，而高程方向均方根误差可达10公分；快速产品之平面方向均方根误差可达6~8公分，而高程方向均方根误差可达15公分；超快速产品观测部分之平面方向均方根误差可达15~20公分，而高程方向均方根误差可达30~40公分；超快速产品预估部分之平面方向均方根误差可达2~3公尺，而高程方向均方根误差可达3~4公尺。

The quantitative and qualitative methodologies were both used in this thesis, andby presently observing of English Immersion classroom teaching and video taperecording, a lot of information about teacher-child speech interaction was collected. Theinternational Child Language Data Exchange System, known as CHILDES, wasadopted to analyze the collected information qualitatively and quantitatively.

本项研究采用量化研究和质性研究相结合的方法，通过对幼儿园大、中、小班英语浸入式教学活动的现场观察和录像收集师幼言语互动语料，运用国际通用的儿童语言数据交流系统——CHILDES，对收集到的师幼互动交流言语数据进行统计分析，在对研究资料进行量化分析的同时，对研究结果进行质性的分析和讨论，并提出了相应的教学建议。

First, the team compared standard morphometric measurements of fossil material collected from Norfolk Island (the first place the birds were eaten up, located east of Lord Howe Island) to modern specimens collected in the northern Tasman Sea and previously presumed to be masked boobies.

科学家小组首先将在诺福克岛（坐落在豪勋爵岛的北部，是这种鸟类第一次被吃光的地方）收集的化石材料与在北塔斯曼海收集的现代品种进行了标准的形态学尺寸对比，初步推测这就是面罩鲣鸟。

Up to now, he has collected a great number of stamps.

到目前为止，他已经收集了大量的邮票。

Subjects include COPD patients, medical and nursing staffs. The study was carried out in a medical ward of a hospital in the northern Taiwan. Data were collected from the control group of 30 patients first, then the experimental group of 30 patients. Both groups of patients were matched with the disease severity.

於北区某区域教学医院内科病房进行收案，先收集对照组30名，再依慢性阻塞性肺疾病病人之疾病严重度配对方式收集实验组30名，医护人员则以26人为样本。

It is an urgent necessity to find a non-pharmaceutical pain control, which can be effectively applied in our people by nursing staff. The purpose of this study was to find the effect of breathing relaxation therapy in reducing pain and physiological responses in cardiac surgical patients. This experimentation collected 60 cardiac surgical patients from the cardiac surgical ward of a medical center.

随著非药物性疼痛处置发展，松弛训练的效益近年来颇受重视与肯定，但国内相关研究，并不多见；然探讨发展适用於国人且又有效的非药物性疼痛处置，以提供护理人员执行相关处置参考，实为当务之急，研究目的在探讨呼吸松弛处置改善心脏手术病患疼痛及生理反应的成效。

The descriptive correlation research design was conducted from Mar. 2004 to Feb. 2005. Patients who had their first CABG surgery were selected by purposive sampling from the cardiovascular ward at a medical center in Taichung. Baseline data were measured by symptom distress scale, self-efficacy in physical function scale and physical function scale, collected one day before operation, ten days after operation, one month and three months after operation, 60 subjects were enrolled in the study.

本研究采描述性相关性之研究设计，从93年3月至94年2月，共计12个月，以立意选样的方式，於台中市某医学中心之心脏外科病房选取初次接受冠状动脉绕道术之病患，以病患基本属性量表、身体症状困扰量表、身体功能状态量表与身体功能之自我效能量表分别於术前1日、术后十日内、术后1个月及术后3个月收集资料，符合收案条件者共计95人，最后完成资料收集者有65人。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。