查询词典 coli

The major route of β-alanine production for pantothenate in Escherichia coli is through the α-decarboxylation of L-aspartate, which is catalysed by the enzyme L-aspartate-α-decarboxylase. The panD mutant of E. coli is β-alanine auxotrophy.

在大肠杆菌中，用于泛酸生物合成的的β-丙氨酸主要是由L-天冬氨酸α位脱羧而产生，这个生化反应由panD基因编码的L-天冬氨酸-α-脱羧酶催化。

This research successfully constructed an engineered E. coli, which could express the catalase gene perA from Bacillus stearothermophilus under the control of the tac promoter, then the fermentation condition of engineered E. coli and purification, properties of its produce catalase were studied.

本文成功构建在tac启动子控制下表达嗜热脂肪芽孢杆菌过氧化氢酶基因perA的大肠杆菌工程菌，并对其发酵条件、重组过氧化氢酶的纯化及酶学性质进行了研究。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

As a result, Core gene was expressed in E.coli with yields of 20% of total proteins and it was a 31KD fusion protein on SDS-PAGE and Western-Blot, it shows that Core protein was highly expressed in E.coli;One fragment of 369bp could be seen on the gel of the Core gene PCR product. A band of 29KD could be seen on the Western-blot after induction of yeast containing pPICZaA-Core for 72 hours. This indicate that Core protein was secretively expressed in the Pichia pastoris with weak yields.

结果显示pBVIL1-Core的表达产物经SDS-PAGE分析出现一条约31KD的带，与预期融合蛋白的分子量相符，表达蛋白存在于包涵体中且表达量占菌体总蛋白的20%，Western-blot显示诱导后菌体在相应位置出现特异性杂交带，表明

No squalene synthase polypeptide of expected molecular mass was observed in E. coli containing the putative full-length squalene synthase cDNA, however, overexpression in E. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus.

但在含有全长的鲨烯合酶cDNA的大肠杆菌中并没有观察到预期大小的鲨烯合酶表达，而C末端截短30个疏水氨基酸的鲨烯合酶可在大肠杆菌中过量表达。

At the same time, intact shuttle-plasmid pZ189 was also introduced into the host cell which was used for detection of nontargeted mutation frequency. After replicating in cells, progeny plasmid DNA was isolated and transformed into E coli. MBM7070.In the presence of ampicillin, X-gal and IPTG, pZ189-transformed E. Coli MBM7070 forms blue colony if the plasmid contains an active SupF suppresser tRNA gene, and white or light blue colony if the SupF tRNA gene is inactive.

但是由于检测非定标性突变需同时引入穿梭质粒pZ189在细胞中复制，并回收质粒DNA，通过转化宿主菌E.coliMBM7070，在含x-gal和IPTG的氨苄指示板上培养，如果用于检测突变的靶基因SupF tRNA基因发生突变，则菌落色泽为白色或淡蓝色，而正常者为兰色。

The infection rate was significantly decreased if we upregulate Attacin expression by E.coli injection prior to trypanosome feeding.Attacin gene was cloned and expressed in Drosophilla S2 cells. Purified protein shows strong activity against E.coli, as well as trypanosome. But tsetse symbiont Sodalis, a gram negative bacterium, was quite resistant to Attacin.

克隆Attacin基因并在果蝇S2细胞中表达，对纯化的Attacin蛋白的研究表明它除了对大肠杆菌有很强的活性外，对锥虫也有较强的活性，而同为格兰氏阴性菌的采采蝇共生菌Sodalis则在试验中表现了较强的抗性。

Core protein, which is very conservative, can induce high level of humoral and cellular immunity. For these years, Core antigen was expressed in E.coli successively. But E.coli is uncapable of managing gene expression and protein procession like eukaryotic cell.

HCV核心蛋白高度保守且有很强的免疫原性，可诱发高水平的特异性体液免疫及细胞免疫，是HCV诊断试剂中必不可少的组分之一。

The result indicated: antibacterial concentration of inner thallus increased progressively with time prolongation when added CCCP with antibacteria, proved that multi-resistance of E.coli in livestock and avian is mediated really by active efflux system; The multi-resistant level have positive correlation with AcrAB transcription and expression level; Quantitive RT-PCR,Western-blotting and fluorescence quantitive PCR can use to detect AcrAB.these results provide theory foundation to detect and study multi-resistance of E.coli in veterinary clinic and have a important scientific significance to develop neotype antibacterials.

结果表明，多重耐药株在有CCCP 存在时，菌体内抗菌药物的浓度随时间的延长而递增，证明畜禽大肠杆菌多重耐药性确实由主动外排系统介导；其多重耐药水平与AcrAB的转录、表达水平呈现正相关；定量RT-PCR、Western-blotting、荧光定量PCR可用于AcrAB水平的检测。这些结果对检测临床大肠杆菌的多重耐药水平、对研究兽医临床大肠杆菌多重耐药机制提供理论依据，对开发新型抗菌药物有重要的科学意义。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失