查询词典 coli

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

In this experiment, Jacob et al analyzed the progeny from matings that are interrupted after different intervals, using different Hfr strains in which F has integrated in different places. Then, it became both conceptually and practically possible to map genes in E. coli simply by measuring and comparing time of transfer.

在此实验中，Jacob等使用不同的Hfr菌株，分析了不同时期被间断交配的细菌子代，根据不同基因被转移的次序绘出大肠埃希菌染色体基因图谱。

Each of the three RNA polymerases contains 12 or more subunits, some of which are similar to those of E. coli RNA polymerase.

原来译文：三种RNA聚合酶的每一种含有12个或更多亚基，其中有些亚基和大肠杆菌RNA聚合酶中的相似。

Results L Staphylococcus and escherichia coli were the main nosophytes. This type of bacterium had hyp...

L型细菌对红霉素、氯霉素、利福平等显示出相对较低的耐药性，使用这些药物提高了疾病的治愈率

In order to study the expression, subcellular distribution and the function of NS2 protein, the coding region of NS2 was amplified from the hindgut tissue of cockroaches infected with PfDNV by RT-PCR and then the recombinant prokaryotic expression vector pET28a-NS2 was constructed. The recombinant plasmid was transformed into E. coli BL21 (DE3) to express the 6×His fusion protein in the bacteria.

为了对该基因进行深入的功能研究，从感染了黑胸大蠊浓核病毒的蟑螂的后肠组织中通过RT-PCR得到ns2基因编码序列，将其构建于原核表达载体pET-28a，转化大肠杆菌BL21(DE3)获得融合表达产物。

Coli DH5α, the positive recombinants were identified with restriction enzyme analysis and DNA sequencing analysis.

将阳性重组子转入大肠杆菌DH5α，用IPTG诱导重组EBP融合蛋白的表达。

Human KDR extra-cellular domain can be expressed in E coli and after being refolded, has an action of binding to VEGF.

KDR基因片段能在QIA表达系统中得到表达，复性后表达蛋白具有与VEGF结合的生物学功能。

Result The pathogenic microorganisms including Pasteurella, Salmonella, gram-positive Escherichia coli, Staphylococcus and parvovirus were isolated from the captured birds such as sparrow, magpie, great tit, goldfinch, houseswallow, red tailed shrike and pigeon.

结果］从捕获的麻雀、喜鹊、大山雀、金翅雀、家燕、红尾伯劳、鸽子等鸟类中分离出了巴氏杆菌、沙门氏菌、革兰氏肠杆菌、葡萄球菌、细小病毒等病原微生物。

These results suggested that in chloroplast ATP synthase, both the N-terminus and C-terminus of the ε subunit show importance in regulation of the ATPase activity. Furthermore, the N-terminus of the ε subunit is more important for its interaction with γ and some CF〓 subunits, and crucial for the blocking of proton leakage. Compared with the ε subunit from E. coli, the chloroplast ε subunit is more sensible to N-terminal or Cterminal truncations.

从这些实验结果中可以看出：（1）叶绿体ATP合酶ε-亚基的N端和C端对于其功能的发挥都很重要；（2）ε亚基N端和C端氨基酸在抑制ATP水解和堵塞质子泄漏两个功能上起着不同的作用；（3）与细菌ATP合酶相比，叶绿体ATP合酶ε亚基N端和C端的缺失对其功能的影响更为显著。

Known sources of E. coli include undercooked beef, sausage, and contaminated produce.

已知大肠杆菌的来源包括没熟的牛肉，香肠，和受污染的食物。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失