查询词典 coli

Methods PCR amplification of drug resistance gene abeM was carried out using chromosomal DNA of Ab 35 and Ab 36 clinical isolates as template. PCR product of abeM gene was sequenced. The nucleotide sequences and deduced amino acid sequences were analyzed by BLAST of NCBI. After cells of Escherichia coli KAM32 transformed with a plasmid carrying the abeM gene PCR product, MICs of various drugs were determined by serial twofold dilution method.

以临床分离的Ab 35、Ab 36染色体DNA为模板扩增abeM基因，对abeM基因扩增产物进行DNA测序及同源性分析；将abeM基因扩增产物与载体连接后转化入大肠埃希菌KAM32感受态细胞中，对转化子进行药敏分析。

To test this hypothesis, we performed experiments using plasmids containing a luciferase reporter with amber, ochre and opal nonsense mutations within the luxB gene in Escherichia coli.

为验证此假设，我们用几个含有大肠杆菌荧光素酶报告基因的质粒进行了实验，这些质粒中的luxB基因分别包含有琥珀色、赭色和乳白色的无义突变。

This plas mid w as transformed into E.coli BL21 strain. The major band of about 31000 fusion prot ein could be detected by SDS-PAGE analysis and the fusion protein is more than 34% of total cellular protein.

该基因被克隆到融合型表达载体pGEX-2T中，重组融合型表达载体nCT/pGEX-2T转化E.coliBL21，培养表达，SDS-PAGE分析可见Mr约31000的融合蛋白带，融合蛋白占细胞内总蛋白的34%。

In general as long as the temperature reaches 90 ℃, the common E. coli, hepatitis B virus and other bacteria have already perished, but we still continue to boil water, this area is to allow water in the calcium carbonate, magnesium carbonate and other ingredients to fully precipitate impurities, and the other On the one hand, because I am using tap water is treated with chlorine, so a certain degree of residual chlorine in water, in the process of boiling water, chlorine and impurities in the water will generate harmful substances called chloroform.

在一般的长期的温度达90℃，普通大肠杆菌，乙型肝炎病毒和其他细菌已经死亡，但我们仍烧开水，这方面是允许的碳酸钙，碳酸镁和其他水成分，杂质充分沉淀，以及对其他一方面，因为我使用的自来水是用氯处理，因此在水中的余氯，在一定程度上开水，氯和水中杂质的过程中，将生成所谓有害物质氯仿。

The reasons for using E.coli system to express HCV non-structure proteins are as follow:(1) Hepatitis C virus infections are serious in China. Using anti-HCV screening assay to control the quality of blood is the main measure of preventing the transmission of HCV.

本研究利用大肠杆菌系统表达丙型肝炎病毒非结构蛋白主要是基于以下考虑：(1)丙型肝炎在我国流行严重，利用HCV检测试剂控制血源质量是现阶段的主要预防措施。

The aerobic plate count was determined before and after these treatments. The results showed that under the experimental condition, Escherichia coli and Staphylococcus aureus were inactived signi...

结果表明：试验条件的超高压处理对大肠杆菌、金黄色葡萄球菌的杀灭效果很好，残活率最大下降了8.48、5.90对数；对生鲜猪肉和芥菜制品中的微生物具有较好的杀灭效果，残活率最大下降了3.45、3.57对数。

This makes the environment a less hospitable place for E. coli and other disease-causing bacteria to breed.

这使得环境不相宜的地方大肠杆菌和其他致病细菌的滋生。

The result was consistent with that of double-disk synergy test. Among 102 strains of E.coli detected using VITEK GNS-506, 41 strains were found to be ESBLs positive.

结果 仪器检测 48株肺炎克雷伯菌中有24株ESBLs为阳性，用纸片协同法对照结果一致。

METHODS By double-disk test to detect the ESBLs of Escherichia coli and Klebsiella pneumoniae and the metallo-β-1actamase of Pseudomonas aeruginosa and Acinetobacter baumannii.

用纸片协同试验分别检测大肠埃希菌和肺炎克雷伯菌的ESBLs，检测铜绿假单胞菌和鲍氏不动杆菌的金属酶。

Compared with the control, acidifiers could reduce the pH value, change the microbiological circumstances and stop the growth of E-coli and improve the quantities of lactobacillus.

试验结果表明，与空白对照组相比，复合酸化剂降低了肉鸡的肠道pH，改善了微生物环境，抑制了大肠杆菌的生长，同时促进了乳酸杆菌的增殖。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。