查询词典 coli

Coli JM109 (DE3) transformant containing the 〓-tagging vector with the gene leuS was approximately 110 times that of JM109 (DE3)(the host strain without the vector). The overproduced 〓-fusion leucyl-tRNA synthetase can be purified to homogeneity under native condition within two hours by one step affinity chromatography with an overall yield of 55%.

高表达的N—端带有6个连续组氨酸的LeuRS经过一步Ni-NTASuperflow亲和层析可以达到90％以上的纯度，产率为55％，纯化时间仅为2小时，而且LeuRS所带的〓-标签对酶的结构和功能都没有影响。

Amid a growing E. coli scare, Topps Meat Company is expanding its recall of frozen hamburger patties.

在对致病性大肠杆菌不断增长的恐慌中，Topps肉类公司扩大了召回冷冻汉堡小馅饼的数量。

Cephalexin is effective against a wide range of Gram positive and Gram negative bacteria including: Staphylococci, Streptococcus spp., Pneumococci, E. Coli, Klebslella spp, and Salmonella spp.

头孢氨苄是有效的针对范围广泛的革兰氏阳性和革兰氏阴性菌包括：金黄色葡萄球菌，链球菌属。，肺炎球菌，大肠杆菌，Klebslella菌和沙门氏菌。

Enzyme activity assay demonstrated that the Trx-BTGase expressed in E. coli could be functional in polymerizing other proteins, and no influence on the biological activities of BTGase to polymerize BSA was found in case of the thrombin cleavage of this fusion protein.

酶活性分析表明表达的Trx-BTGase融合蛋白具有交联蛋白的活性，并发现Trx-BTGase融合蛋白和经凝血酶酶切后得到的BTGase单体都能催化牛血清白蛋白的聚合反应。

Then the inhibition effects of the produced sulfated glucan on E. coli in vitro and in vivo were studied.

利用得到的硫酸化葡聚糖进行体外和体内抑制大肠杆菌效果研究。

API-B has been successfully expressed in both yeast and E. coli system, and the expression products have been purified to homogeneity. By the method of deletion mutagenesis, it was proved that the intact N- and C- terminuses of APIB were necessary for the inhibitory activity, consistent with the tertiary structures of the inhibitors from the STl superfamily.

成功地建立了API-B在酵母及大肠杆菌体系中的表达方法和纯化路线，并通过缺失突变的研究，证明API-B的N-端及C-端附近序列对于维持分子的高级结构和抑制活性非常重要，该结果与STI抑制剂超家族成员的结构特征相一致。

This anion pump is composed of two subunits, the catalytic 63KDa ArsA, a hydrophilic protein, when purified, exhibits oxyanion-stimulated ATPase activity, the hydrophobic 45. 5KDa ArsB protein, located in the inner membrane of E. coli, is the putative anion translocating channel and also serves as the anchor for ArsA to attach on the membrane.

ArsATP酶由水溶性的63KDa的ArsA蛋白和膜整合的46KDa的ArsB蛋白组成，ArsA蛋白是催化亚基，具有亚砷酸盐和亚锑酸盐激活的ATP酶活性，ArsB蛋白则构成转运亚砷酸盐和亚锑酸盐的阴离子通道，同时作为ArsA蛋白在膜上的结合位点。

The settling may after joining the TE buffer solution (10m-MTris-HCL lmMEDTA, pH8.0) on the molecular sieve live in the protein, goes to RNA; May also use the ultracentrifuging to go to the protein to occupy, goes to RNA, goes to level shape DNA or the DNA fragment.

E.coli→用溶菌酶去细胞壁→用表面活性剂如SDS、Trit X-100等EE细胞膜→用乙酸锅使DNA、RNA及蛋白质大部分沉淀(90%以上)。

And starting tomorrow, the government will allow food producers to start zapping fresh spinach and iceberg lettuce with just enough radiation to kill E.coli and other dangerous germs.

从明天开始，政府将会允许食品生产者对菠菜和卷心莴苣使用适当的辐射来杀死E大肠杆菌和其他的一些危险的细菌。

The substance extracted showed some antibiotic activity against Staphylococcus aureus, Bacillus subtil, Escherichia coli, Aspergillus niger, penicillium, the minimum inhibitory concentration was 0.0625, 0.0625, 0.0313, 0.125, 0.25 mg/ml, and minimum bactericidal concentrations was 0.25, 0.125, 0.0313, 0.25, 0.5 mg/ml respectively.

该提取液对金色葡萄球菌、枯草杆菌、大肠杆菌、黑曲霉、青霉都有明显的抑菌效果，最低抑菌浓度分别为：0.062 5，0.062 5，0.031 3，0.15，0.25 mg/ml；最小杀菌浓度为0.25，0.125，0.031 3，0.5，0.25 mg/ml。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。