查询词典 coli

Results Causing the pathogen of the infection regard the Green's surname feminine gender rod bacteria as principle,occupying 90.2%,the escherichia coli is the most familiar to occupy 65% the next in order for transform the rod bactenia,viceescherichia coli,sewerage intestine rod bacteria,etc.of aerobacter aerogenes.

结果：引起感染的病原体以革兰阴性杆菌为主，占90.2%，大肠埃希菌最常见占65％，其次为变形杆菌、副大肠杆菌、阴沟肠杆菌、产气杆菌等。

Coll or MPAIV alone.the group inoculated with MPAIV and E.coli was more severe inflammation of the trachea, lung, and air sac and dispersion necrosis of parenchyma organs than that of groups inoculated with MPAIV or E.coli alone.

迅速，细菌数目也比A组多，l2 细菌数目差异显著，在3h后差异不显著。④各组脾脏。肾脏和肝脏在接种后偶尔可以分离到细菌，而且数量很少。

objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase，sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶，按其结合的金属性离子根据其中金属辅基的不同可分为4类：cu/zn-sod、mn-sod、fe-sod和ni-sod，它们通过催化超氧阴离子自由基0-2发生歧化反应，达到清除o-2的效果，具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效，广受国内外科研工作者的关注和重视[1]。

An anaerobic-aerobic intermittent CSTR process was used to decolorize mixed dye with E. coli NO3 strain at a hydraulic retention time of 32, 18, and 10 h. The color-removal efficiency was around 70 ~ 80%. Under steady operation for over 117 h, the total decolorization was 10900, 18200, and 7590 for HRT=32, 18, and 10 h, respectively. After repeated operation cycles, the decolorization rate of E. coli NO3 was always higher than the original cycle. This may be attributed to the acclimation effect since the E. coli NO3 cells were repeatedly exposed to the azo dye.

NO3於HRT = 10、18、32小时之条件下进行厌氧-好氧间歇式CSTR生物反应器褪色操作，褪色率可以达到7080﹪左右；在约117小时的稳定褪色操作下，总褪色量分别为10900、18200及7590，这显示NO3在长时间之间歇式CSTR褪色操作下，不但稳定且具有优异之褪色能力；若将一组好氧、厌氧步骤视为一个循环去探讨褪色率之变化，则可观察到随著循环次数增加其褪色率逐次上升，之后则明显下降，这与摇瓶重复批次实验相似之结果，也可以解释为驯养效应所导致。

The multi-drug resistance rate of Salmonella and E.coli were 91.80% and 100%.According to the genes sequence published in Genbank ,we analysed the value of Tm ,content of G+C, oligonucleotide dimer by DNAStar and Primer 5.0 software, and according to the construction of I integron ,we designed 3 pairs of primers to amplify intIl gene ,variable segment ,3"-conserved segment for detectting Salmonella and E.coli"s I integron.As a result , aimed genes were amplified and sequenced;The detection rate of I integron of 61 Salmonella and 48 E.coli were 81.97% and 66.67%.

据文献报道及GenBank中注册的基因序列，运用DNAStar软件分析同源率及Primer5.0软件对Tm值、G+C含量、引物二聚体等进行分析，根据Ⅰ类整合子的结构设计了3对引物，对Ⅰ类整合子的intI1、可变区、3'保守端进行扩增，检测沙门氏菌和大肠杆菌Ⅰ类整合子；扩增出了相应基因片段并测序；61株沙门氏菌和48株大肠杆菌中分别有50株和32株菌检测出Ⅰ类整合子，Ⅰ类整合子的检测率分别为：81.97％和66.67％。

objective to study the risk factors on esbl producing strains of k.pneumoniae and e.coli.methods aprospective survey on esbl producing strainsof k.pneumoniae and e.coli for a36-month period.results the resistance rates ofe.coli were:98.08%for ctx,73.08%for amc.the resistance rates of k.pneuˉmonialwere;95.74%for ctx,97.87%for amc.the use rate of third generation cephalosporins was much higher than those of esbls nonproducing strains（p.05）.it induce esbls that3rd generation cephalosporins were used extensive.after strenghtening the antibiotic controls,esbls detection rate has gone down.conclusion the factors of infection of esbl-producing strains were the severity diseases,cellular immunological condition,improper medical manipulations.to prevent esbl-producing strains,reasonable antibiotics usage may be the effective measure.

目的 分析临床大肠埃希菌及肺炎克雷伯菌产esbls的危险因素，并加以控制。方法前瞻性监测产esbls菌的情况，并对感染者进行临床调查。结果产esbls的大肠埃希菌、肺炎克雷伯菌的耐药率头孢噻肟钠为98.08%和95.74%，阿莫西林+棒酸为73.08%和97.87%，产esbls菌感染者头孢第三代的使用率（70.21%）显著高于非产esbls菌感染者（39.47%）（p.05）；第三代头孢的大量使用诱导esbls的产生。通过加强抗感染药物的使用管理esbls检出率开始下降。结论严重的基础病、高龄、机体免疫力低下，长期住院者是esbls菌感染的易感宿主，皮质激素、化疗及介入性疗法是esbls感染的高危因素。滥用抗感染药是产生esbls的重要因素，合理使用抗感染药是防止esbls产生的主要措施。

Induced by 4℃ low temperature, Escherichia coli inoculated in water can enter the viable but nonculturable state; in the room temperature, Escherichia coli may keep culturable state for a long period; eutrophic culture media of TSA can produce more bacteria count compared with oligotrophic culture media of PCA; evaluating the bacteria count Escherichia coli in water by plate count method may underestimate the risk of intestinal pathogen contamination.

在4℃低温诱导下，大肠埃希菌在水中可进人活的非可培养状态；在室温下，大肠埃希菌在水中可保持较长时间的可培养性；富营养的TSA琼脂检测的菌落数多于寡营养的PCA琼脂；用平板涂布法检测水中的大肠埃希菌可能低估了肠道致病菌污染的风险。

The rewards are identical, however. Both Homo sapiens and E. coli are elite cosurvivors. And neither particularly has an advantage over the other in surviving the next million years. Actually, some pessimists give E. coli 100-to-1 odds on outliving humans, even though E. coli can currently live only in our guts.

事实上，在大肠杆菌和人类谁更长寿的赌局上，许多悲观主义者给出大肠杆菌100比1的赔率，尽管现在，这种微不足道的生物只能生存在我们人类的身体里。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Coli disease. And the use of allicin sulbactam or amoxicillin by a certain percentage for the Joint duck E.coli disease prevention observed experimental results, the results showed that: Allicin, amoxicillin, and sulbactam sodium single drug to duck the E. coli O0701 MIC were 1024ug/mL.Compound MIC its portfolio of 3～4.5ug/mL than single drug decreased by about 64 times.

利用大蒜素与阿莫西林或舒巴坦钠按一定的比例联合用于鸭大肠杆菌病的防治观察实验效果，结果显示：大蒜素、阿莫西林、与舒巴坦钠单药对鸭大肠杆菌 O0701的 MIC 均为1024ug/mL ，其复方组合 MIC 为3~4.5 ug/mL ，较单药降低了约64倍。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失