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MBC value was 0.61, 0.304, and 0.00475 μgmL^(-1) respectively. Sterilization rate of ceftiofur suspension was the fastest to Actinobacillus, followed by Streptococcus. PAE of ceftiofur suspension was relatively short to E. coli, which illustrated that it was nonconcentration dependent. PAE was obvious longer to Actinobacillus, which showed that it was concentration dependent, however, it revealed part concentration dependent to streptococcus.

头孢噻呋混悬剂对大肠杆菌、放线杆菌及猪兽疫链球菌的MIC值分别为：0.076、0.304、0.00475μgmL^(-1)；MBC值分别为：0.61、0.304、0.00475μgmL^(-1)；头孢噻呋混悬剂对放线杆菌的杀菌速率最快，对链球菌次之；头孢噻呋混悬剂对大肠杆菌PAE较短，呈现非浓度依赖性，对放线杆菌的PAE较长，呈明显的浓度依赖性，而对猪兽疫链球菌呈现部分浓度依赖性。

Objective To express and characterize the C terminal fragment of HEV ORF2. Methods The gene encoding C terminal 128 amino acid fragment of HEV ORF2 was inserted into nonfusion expression vector pBV220. The resultant pBVORF2.3 was transformed into E.coli strain DH5α.

用pBV220载体进行抗原的非融合表达，包涵体经变性凝胶过滤层析及离子交换层析纯化后透析复性，以Western blot、ELISA、动物免疫与抗原捕获RT-nPCR等方法研究其免疫学特性。

Glioma is still one of refractory disease in the neurosurgical field; the development of new primary and adjuvant treatment is vital. Recently, the gene therapy of glioma is developed rapidly and there are many methods about the gene therapy that include: suicide gene therapy, immunologic gene therapy, drug resistangce gene therapy, angiostatin gene therapy and so on. The sucide gene therapy is the most potential approach of antitumer, these nonmammalian genes encode enzyme that convert nontoxic prodrugs into highly toxic metablites. Cells transfected with suicide genes are targeted for specific negative selection, witch can be induced by administrtion of the corresponding produg. Among the enzyme/produg combinations, two of the best characterized system are herpes simplex virus thymidine kinase /ganciclovir and Escherichia coli cytosine deaminase /5-flourocytosine (5-FC). The formor can convert the antiviral nucleoside analogs acyclovir , ganciclovir to their nucleoside monophosphate derivatives, the monophosphate forms are subsequently phosphorylated by endogenus cellular kinases to triphosphates, these molecules are potent inhibitors of DNA synthesis.

近年来脑胶质瘤的基因治疗发展迅速，应运而生的方法有自杀基因、免疫基因、多药耐药基因以及抗血管生成基因等，其中自杀基因被认为是最有前景的基因治疗方法，它又称病毒介导的酶／药物前体疗法，是利用转基因技术将哺乳动物细胞中所不含有的自杀基因转入到哺乳动物肿瘤细胞中，该基因表达的产物可将无毒的药物前体转化为毒性药物，从而选择性杀伤该肿瘤细胞，常用的自杀基因有单纯疱疹病毒-胸苷激酶基因和大肠杆菌胞嘧啶脱氨酶基因，前者催化无毒性抗病毒核苷类似物如丙氧鸟苷、无环鸟苷等成为单磷酸核苷衍生物，然后在内源性细胞激酶作用下转化为具有明显毒性的三磷酸核苷，作为DNA合成链的终止剂和DNA合成酶的抑制剂，干扰细胞DNA的合成；后者编码的胞嘧啶脱氨酶可催化5-氟胞嘧啶（5-FC）脱氨成为5-氟尿嘧啶（5-FU），然后代谢为有毒性的5-氟尿嘧啶-5′三磷酸（5-FUTP）和5-氟-2′脱氧尿嘧啶-5′磷酸（5-FdUTP），5-FUTP通过与UTP竞争性结合而抑制mRNA和tRNA的合成，5-FdUTP则作用于胸苷合成酶，导致TMP衰竭而阻止DNA的合成，最终诱导肿瘤细胞凋亡。

The results showed that the main pathogenic bacteria ledlocal cows to endometritis were streptococcus,the next,Escherichia coli,staphylococcus and pseudomonas aeruginosa.NFPL could quickly reduce thenumber of the bacteria in uterus,and strongerly inhibit these bacteria in vitro.NFPL could excit the uterus,which occurred in enlarging contractionamplitude.NFPL could lower abnormal high content of progesterone and raiseabnormal low oestradiol.

结果表明，引起本地奶牛子宫内膜炎的主要致病菌是链球菌，其次是大肠埃希氏杆菌，葡萄球菌，绿脓杆菌；新促孕液能能迅速减少病牛子宫内细菌的数量，在体外对这些细菌也有较强的抑制作用；新促孕液对兔子宫有明显的兴奋性作用，主要表现在收缩波幅的加大；新促孕液能降低病牛血清异常升高的孕酮含量，升高异常降低的雌二醇含量。

College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,ChinaAbstract: To obtain the hybridoma cell line specific to 45W-4BX antigen of Taenia solium oncosphere,the recombinant plasmid pGEX-4BX containing TSO45W-4BX gene was highly expressed in Escherichia coli induced with IPTG.

为获得针对猪带绦虫六钩蚴45W-4BX抗原的杂交瘤细胞株，用IPTG对TSO45W-4BX的重组质粒pGEX-4BX进行了诱导表达，并采用Sepharose-4B层析技术对表达产物进行纯化，运用SDS-PAGE和Western-blot分别对其纯度和活性进行了检测。

In chapter 6, the recognition of ORF and exon in several representative lower organisms, E. coli, S. cerevisiae and C.

第六章讨论了大肠杆菌、酵母和线虫这几种低等生物中ORF和外显子的识别。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落，提取重组质粒，经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定，成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23～244)]和pMYO2[Ov rPrP(91～244)]。

Objective To overexpress human acidic fibroblast growth factor in Escherichia coli and study the biological activities of recombinant aFGF.

目的：探讨人酸性成纤维细胞生长因子（acidic fibroblast growth factor， aFGF）在大肠杆菌中高效表达的方法及重组人aFGF的生物学活性。

According to the sequence and functional analysis result some ORFs was selected to overexpress in E. coli. Cloning of the endogenous plasmid SLP3 of S.

随后根据测序的结果以及复制区和接合转移功能区鉴定的结果，选择对一些重要的功能蛋白进行在大肠杆菌中的过量表达。

AIM: To construct,overexpress and purify a stable and active form of shortened human acidic fibroblast growth factor in Escherichia coli.

目的：稳定且具有活性的截断型人酸性成纤维细胞生长因子的构建、表达及纯化。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。