查询词典 coli

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

Three kinds of BCRC No.51534, 10322 and 10675 would be selected and acted as an experimental sample of Escherichia coli. Results shows that Escherichia coli of No.51534 will appear better performance because the maximum of open circuit voltage, closed current and power density are 1.01V, 22mA and 1342mW/m2, respectively. Concerning the effect of culture time with respect to different phase type on the electricity performance of MFCs, the time points on the intersection between lag phase and logarithmic phase, the middle of point of stationary phase for growth curve of Escherichia coli would appear a good performance of MFCs. In addition, the BCRC No. 51534 Escherichia coli possessing a better performance of MFCs than others would be suggested and applied to further studying. Comparison with the performance of MFCs with respect to electron mediator under different mole number, result shows that electron mediator of methylene blue with 4.63mM would appear a better electricity performance of MFCs than others. Concerning the different material of proton exchange membrane with PTFE-Nafion, Nafion 211, 212 and 117 with respect to the performance of MFCs, result shows that the Nafion 117 applied in MFCs will have a better performance of MFCs than other cases. Finally, the effect of molar concentration on the performance of MFCs would be expected at the studied cases of 0.4M, 0.2M, 0.1M and 0.05M respectively for cathode oxidant, result shows that a good performance of MFCs will happen at the condition of 0.2M. Those observations will be useful to improvement of MFCs in the further study.

於上述电池系统条件下，进行大肠杆菌生长曲线、电子传递介质、质子交换膜、电极与阴极氧化剂对电池电性效能分析；选择编号10322、10675与51534之大肠杆菌为实验菌株，依定量培养之生长曲线取出代表不同时生长特性时期的培养时间，利用亚甲基蓝作为电子传递介质进行实验分析从所测得的电量进行分析，以编号51534之大肠杆菌的微生物燃料电池有最大的开路电压为1.01V及最大闭路电流为22mA；当极化曲线中电压为0.47V、电流为11.4 mA时有最大的功率密度为1342 mW/m2；加以负载有平均工作功率密度294 mW/m2；从生长曲线与电性效能来分析，得知生长曲线的迟滞期与对数期的转变点与静止期的中间点有最佳电性效能表现；对於加入不同莫耳数之电子传递介质methylene blue、neutral red与thionine之电池效能表现，则以加入4.63mM methylene blue电子传递介质的电池有较佳平均功率密度230 mW/m2；另对於质子交换膜PTFE-Nafion、Nafion 211、Nafion 212与Nafion 117之电池效能表现，以Nafion 117质子交换膜的电池有较佳平均功率密度340 mW/m2；对於分析加入不同莫耳数浓度0.4M、0.2M、0.1M与0.05M的阴极氧化剂之电池效能，则以0.2M的阴极氧化剂的电池可得到较佳平均功率密度429 mW/m2。

Firstly,we isolated clinically and induced artificially multi-resistant Escherichia coli,detected antibacterials intake by different resistant level strains with CCCP and fluorescence spectro method and ascerained existing active efflux system in this strains; Secondly, construced internal standard of quantitive RT-PCR and detected AcrAB mRNA level of different resistant E.coli with quantitive RT-PCR; Thirdly, prepared AcrA antibody and detected AcrAB protein level of different resistant E.coli; eventually,designed and constructed Taqman probe of AcrAB and detected AcrAB level of different resistant E.coli with fluorescence quantitive PCR.

临床分离及人工诱导大肠杆菌多重耐药株，氰氯苯腙结合荧光分光法检测不同耐药水平菌株对抗菌药物的摄入，确证主动外排系统的存在；构建了定量RT-PCR的内标准DNA，通过定量RT-PCR检测动物源性大肠杆菌AcrAB mRNA的水平；制备AcrA抗体，采用Western-Blotting检测不同耐药株AcrAB蛋白的表达水平；设计和构建AcrA、AcrB的Taqman探针，检测不同耐药菌株AcrAB的水平。

The adhesion test showed that small intestinal epithelium cells of 30-35-day-old postweaning piglets with both genotype M307~ and M307~ could adhere to the standard E.coli strain express F18ab fimbriae, the recombinant E.coli express F18ac rE.coli1534 and the recombinant pnirBMisL-fedF E.coli displaying FedF subunit on the surface of E.coli, but small intestinal epithelium cells with genotype M307~ and 3-day-old piglet could not adhere to the three kinds of bacteria descried above, the latter with good adherence capability of 987P fimbrial E.coli.

9仔猪小肠上皮细胞的黏附试验结果显示，30～35日龄M307~基因型和M307~基因型断奶仔猪的小肠上皮细胞均能与表达F18ab菌毛标准菌株107／86、诱导表达F18ac菌毛的重组大肠杆菌rE.coli1534及诱导菌体表面展示表达F18黏附亚单位FedF的重组大肠杆菌pnirBMisL-fedF发生黏附作用，而同日龄M307~基因型断奶仔猪的小肠上皮细胞均不能与上述三株大肠杆菌发生黏附作用。3日龄易感仔猪小肠上皮细胞也不能黏附上述三株大肠杆菌，但可以很好地黏附表达于987P菌毛的大肠杆菌。

Objective To optimize the expression condition of selenocysteine by controling the quantity of the four gene products in Escherichia coli expression of selenocysteine:SelA,SelB,SelC and SelD.

在Escherichia coli中，Sec的表达效率只有正常氨基酸的1%～3%［3］。

We found that neither the fusioned nor the non-fusioned forms of Hu-P68 cDNA open reading frame could express efficiently in the E. coli cells; in different prokaryotic expression vectors and under various promotors resulted in no distinctive variations. Deletion of 5'UTR by synthesis adaptors, deletion of 3'UTR or signal peptide sequence by PCR gained no gross improvement. Only the truncated API-I proteins were observed to be highly expressed when the 3'ORF fragment of the Hu-P68 cDNA was fusioned at the 3'end of PAI-I gene.

结果发现无论是以融合形式存在，还是以非融合形式存在，人的P68 cDNA基因在E.coli细胞中都未能获得高效表达：在不同表达载体中，不同启动子对P68 cDNA基因的表达没有显著差异；利用合成Adapter的方法删除5'UTR，及利用PCR技术删除3'UTR和信号肽序列对表达没有明显改善；分别表达被分割的P68 cDNA5'端编码区、中央编码区和3'端编码区均未见有明显表达；当P68 3'端编码区与高效表达蛋白PAI-Ⅰ基因3'末端融合时，仅见有PAI-Ⅰ以截断形式高效表达出来。

Coli DH5α and Property of the pelA

coli中的表达和酶学性质研究

To elucidate the evolution of 16S rRNA, we succeeded in replacing the 16S rRNA gene of an E.coli strain with all rrn operons deleted with the 16S rRNA gene of Bacillus subtilis.

为了进一步研究16S rRNA的进化，本文将Escherichia coli中最后一个拷贝的16S rRNA基因替换为Bacillus subtilis的16S rRNA 基因，得到了菌株SQ110BSX。

Plackett-Burman design was used to evaluate the effects of eleven factors for succinic acid production by Escherichia coli NZN111.

采用Plackett-Burman设计法(Plackett-Burman， PB)对影响Escherichia coli NZN111厌氧发酵生产丁二酸的11个因子进行了筛选。

Moreover, the product refolded from inclusion bodies of E. coli shows the predominance in homogeneity and the lower cost.

说明E。 coli表达体系可生产质量均一、高生物学活性的rhNGF-β，且具有明显成本优势。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失