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The gene of human proinsulin mutant (A21Asn→Gly,B17Leu→Ile,B31Arg,B32Arg,GIRR ) was synthesized and recombinated to the fusion protein expression vector pTXB1 and expressed in E.coli BL21(DE3) with high protein level.

构建人胰岛素原突变体GIRR（A21Asn→Gly，B17Leu→Ile，B31Arg，B32Arg）基因的原核融合表达载体并进行表达和纯化，进一步对其性质进行研究。

Results:The results showed that rate of the drug fast is very high. As the bacteria distribution ,the main pathogenic bacteria was the gram-negative bacilli(65.33 %), the second pathogenic bacteria was the gram-positive cocci(18.29%). and the mycetes(16.38%) occupied the third. In gram-negative bacilli the most were Pseudomonas aeruginosa,Acinetobacter baumannii,Klebsiella pneumoniae and Escherichia coli;Staph is the main pathogenic bacteria in gram-positive cocci;and in mycetes the main pathogenic bacteria were Candida albicans and Candida glabrate.

结果：EICU医院感染病原菌多为耐药菌，病原菌以革兰阴性杆菌为主占65.33％、革兰阳性球菌第二占18.29％、真菌第三占16.38％；革兰阴性杆菌中以铜绿假单胞菌、鲍氏不动杆菌、肺炎克雷伯菌及大肠埃希菌为主；革兰阳性菌主要以葡萄球菌属为主；真菌以白假丝酵母、光滑假丝酵母菌为主。

PGAD424 that has the activation domain of yeast'transcript activation factor GAL4 is a kind of shuttle vector that could replicate in both E. coli and yeast. One-sixth recombinant plasmids of this library could express fusion protein of GAL4-AD and proteins from Heliothis armigera.

通过在合成cDNA第一链时参入α〓PdCTP，显示出合成的cDNA为6.0Kb以下的连续区带，测得文库的滴度为1.5×10〓，可以代表亲本细胞的全部编码蛋白质的基因序列，即该文库具有代表性。

It could apparently inhibit the growth of Escherichia coli and Helminthosporium turcicum Pass with the eight tested bacteria and plant pathogeny fungi by testing filter paper wafer.

用滤纸圆片法检测，BOL在测试的8种菌中对大肠杆菌和玉米大斑病菌的生长有抑制作用。

Coli in patients with acute leukemia after chemotherapy and related nursing. Methods 11 acute leukemia inpatients with hematosepsis caused by ESBLs positive E.

方法回顾分析11例急性白血病并发ESBLs阳性大肠杆菌败血症患者抗生素使用情况、药敏结果、中性粒细胞计数及转归。

Hemoclastic Escherichia coli was the reason of this disease. And surroundings sanitation, feedingstuff, management, microelement, stress factors were main inducing factors.

结论］溶血性大肠杆菌是引发猪水肿病的病原，环境卫生、饲料、管理、微量元素、应激因素等是该病的主要诱发因素。

Results 761 strains were isolated from 722 patients (50.1%) of which, 170 (22.3%) were Klebsiella pneumoniae, 130 (17.1%) were Escherichia coli, 89 (11.7%) were Streptococcus pneumoniae, 63 (8.3%) were Staphylococcus aureus, 60 (7.9%) were Hemophilus influenzae and Hemophilus parainfluenzae.

结果 1441例病人中，722例检出细菌共761株，分离阳性率为50.1%，分离菌依次为肺炎克雷伯菌170株(22.3%)、大肠埃希菌130株(17.1%)、肺炎链球菌89株(11.7%)、金黄色葡萄球菌63株(8.3%)及流感和副流感嗜血杆菌60株(7.9%)。

93 Target gene were detected by Tem-PCR from 75 specimen, they were: Hemophilus influenzae 40, Streptococcus penumoniae 36, Acinetobacter baumannii 10, Pseudomonas aeruginosa 4, Staphylococcus aureus 3, the other 9 kinds of bacterium including Escherichia coli、Klebsiella pneumoniae and Enterobactor cloacae were not detected by Tem-PCR, the positive rate of Tem-PCR was 39.9%(75/188).(3) For the 14 kinds of bacterium designed by Tem-PCR, compared with the culture, the sensitivity、specificity and coincidence of Tem-PCR is 51.0%, 68.0%, 58.3% respectively.

2经Tem-PCR技术扩增后，188例标本在Luminex100多功能悬浮点阵仪中有75例呈阳性，共检测出93株病原菌的靶基因，分别是流感嗜血杆菌40株，肺炎链球菌36株，鲍曼氏不动杆菌10株，铜绿假单胞菌4株，金黄色葡萄球菌3株，另外9种Tem-PCR已设计的细菌包括肺炎克雷伯菌、大肠杆菌、阴沟肠杆菌等均未检出，Tem-PCR的阳性率是39.9%(75/188)。

The detection rates of methicillin-resistant S. aureus and penicillin-nonsusceptible S. pneumoniae were 15.9% and 84.3%, respectively. The prevalence of ESBLs-producing strains in Klebsiella pneumoniae, Escherichia coli, Serratia spp and Enterobacter cloacae were 31.2%, 46.2%, 94.8% and 16.8% respectively. The resistance rates of Hemophilus influenzae and Hemophilus parainfluenzae to ampicilin were 40% and 36% respectively.

耐甲氧西林金黄色葡萄球菌检出率为15.9%；对青霉素不敏感的肺炎链球菌（包括PISP和PRSP）检出率为84.3%；肺炎克雷伯菌、大肠埃希菌、粘质沙雷菌和阴沟肠杆菌产ESBLs的检出率分别为31.2%、46.2%、94.8%和16.8%；流感嗜血杆菌和副流感嗜血杆菌对氨苄西林的耐药率为36%和40%；铜绿假单胞菌和鲍曼复合不动杆菌对亚胺培南的耐药率分别为10.7%和13.2%。

After the NK gene from recombinant plasmid pUC19-NK was cloned on expression vector pBV220, it was transformed into host cell E. coli HB101. Recombinant plasmid pBV220-NK was extracted for identifying by analysis of restriction enzymes, PCR and sequencing. Studies on recombinant strain's growth and expression showed that heterogene have no distinct effect on host cell. The recombinant plasmid have excellent segregational stability but low structural stability. The results of kinetics of pBV220-NK expression in E.

将重组质粒pUC19-NK上的纳豆激酶基因成功克隆到表达载体pBV220上，并将之转入大肠杆菌HB101中，得到转纳豆激酶基因工程菌，提取重组质粒经单酶切、双酶切及PCR分析验证后，对重组菌的生长及表达进行一系列研究，结果表明：外源纳豆激酶基因的导入对宿主的生长没有明显的影响：重组质粒的稳定性实验表明：该质粒具有良好的分离稳定性，而结构稳定性较差。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。