查询词典 coli

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

2Ai. In addition, cell extracts from E. coli JM109(DE3) cells harbouring the recombinant plasmid pAM28 were able to decarboxylate the histidine present in the reaction to histamine, whereas extracts prepared from control cells containing the vector plasmid alone did not.

此外，从大肠杆菌细胞提取物大肠杆菌JM109中（DE3）中细胞重组质粒pAM28窝藏能够decarboxylate在本组氨酸为组胺反应，从控制细胞制备含有载体质粒提取物，而仅仅没有。

Using microorganism fermentation cultivation technique, with escherichia coli strains of ASP - 311 for fermentation, with rich fumaric acid as the substrate to ferment produce l-aspartic acid, Study the result of activated carbon, and the temperature, the use of bleaching time and discoloring pH value of various conditions, aspartic acid decoloring effect, and finally: the concentration of carbon, decoloring temperature of 1.0% 60 degrees, the bleaching time for 0.5 h, pH for 6, in this condition, the result is the absorbency of decolour 3.2%, to achieve the best effect.

本文利用微生物发酵培养技术，以大肠杆菌ASP-311为发酵菌株，以富马酸为底物来发酵生产天冬氨酸；研究了发酵液中活性炭的使用量、脱色的温度、脱色的时间以及pH值等各种条件对L-天冬氨酸脱色效果的影响，最终得出：活性炭的浓度为1.0%，脱色温度为60℃，脱色时间为0.5h，pH为6，在此条件下，发酵液的吸光度为3.2%，此时的脱色效果达到最佳。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.

目的 将人组织激肽释放酶（基因命名为KLK1，酶命名为hK1）cDNA克隆入大肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株。

Results In animal study, lumbricus could inhibit the growth of staphylococci, bacillus coli and bacillus aeruginosus. The time of wound healing in experimental group was 4 days shorter than that in control group. At 4d and 7 day the numbers of the capillary, blood vessel endodermis and desmohemoblast desmocyte and splitting epithelium of trial group were much more than those of control group. At 4d the trial group′s numbers of splitting mesenchymal cell were much more than that of control group. From 3d on the wound healing and granulation filling of experimental group were much quicker than those of control group.

结果 动物实验中，地龙能抑制金黄色葡萄球菌、大肠杆菌及绿脓杆菌的生长；实验组创面愈合时间较对照组提前了4天；第4、7天实验组动物创面的毛细血管数、血管腔内皮细胞数及间质成纤维细胞数均较对照组明显增多，上皮细胞分裂象也高于对照组，第4天的间皮细胞分裂象也高于对照组；自第3天始创面愈合及肉芽充填速度实验组明显快于对照组。

Results: From the preliminary study, Desmodium gangeticum inhibited the biting and licking behavior induced by formalin in a dose-dependent manner. Additionally, it showed that Desmodium gangeticum could completely inhibit the growth of Pseudomonas and partly inhibited E. coli. in higher concentration. However, it had no effective on the Klebsiella species.

结果：预试实验中发现，大叶山蚂蝗粗抽物对於福马林所诱导小鼠产生的疼痛行为具有剂量依存性的抑制，在较高的浓度下，几乎可完全地抑制绿脓杆菌的生长及部分的大肠杆菌，但对克雷白氏菌则无影响。

Our approach has been applied to both artificial data set and data set of Desoxyribonucleic acid Repair System of Escherichia coli.

并分别用人工数据和大肠杆茵DNA修复系统基因数据进行实验。

The study on the expression of TGF β1 gene in Escherichia coli, not only can provide great amounts of TGF β1 for its clinical and basic research, and also will bring some new ideas and means for the research of other dimeric growth factors.

开展TGFβ1基因在大肠杆菌中表达的研究，不仅有可能获得大量具有生物学活性的TGFβ1产品，从而为TGFβl深入的基础和临床研究提供可能；而且可能为其他双体生长因子的原核表达提供新的思路和方法。

The putative endolysin gene was clone to Escherichia coli, and protein was expressed and purificated.

本实验将此预测之溶菌酶基因转殖於大肠杆菌，进一步表现与纯化此蛋白质。

On the other hand, the protein also showed selective bactericidal activity. Among the tested strains, Escherichia coli is the most sensitive to the protein, Klebsiella pneumoniae is the second, and Bacillus subtilis is the least sensitive to the putative endolysin.

另外，此蛋白质的杀菌效力具有选择性，受测菌株中，对於大肠杆菌的杀菌效力最佳，其次为克雷伯氏肺炎杆菌，对於枯草杆菌效果较差。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。