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coli相关的网络例句
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Coli.Methods The cDNA encoding CP was amplified by RT-PCR using the mRNA extracted from premature Carica papaya as a template,and inserted into vector pET22-b.

方法从生番木瓜中提取mRNA后进行反转录,得到木瓜凝乳蛋白酶的cDNA,PCR产物克隆到pET22-b载体中,重组质粒转化至大肠杆菌BL21,进行诱导表达及表达产物的检测。

Coli and the disruption of pcaH in strain NCIMB 10467 has lead to loss of its ability to catabolize protocatechuate.

而pcaH在NCIMB 10467菌株中的敲除则使该菌完全丧失了代谢原儿茶酸的能力。

Of special interest is a suigeroxide dismutase[7] from E.Coli which contains Mnat an unknown binding site and redox catalyses the disproportionation of O2 to yield O2 and H2O.

Of特殊利益是suigeroxide氧化物歧化酶[7从大肠杆菌]其中载锰在一个未知的结合部位和催化氧化还原的氧产生歧化O2和H2O。

It has been reported that F185K and C280S mutations of HIV1 integrase would improve the enzyme solubility, and the catalytical activity of the enzyme was the same as that of the wildtype enzyme in vitro.

coli中进行整合酶基因表达,SDSPAGE鉴定表达产物,亲和层析纯化蛋白,酶联免疫吸附实验方法测定整合酶的生物学活性。

Coli BL21(DE3) with the amount of 11.49% through thin layer chromatography scanning analysis. Cavia cobaya was immunized using the purified cytorrhyctes to produce the anti-serum. Through the determination of the optimum working conditions, the sensitivity test, the specificity test, repeatability test and sample simulation test, the indirect ELISA method was established, which is a broad-spectrum, rapid and specific to detect various of food-poisoning Vibrio simultaneously.

融合毒素蛋白包涵体经纯化,免疫豚鼠制备血清抗体,确定间接ELISA的最佳工作条件,并进行敏感性、特异性、重复性及模拟样品检测试验,建立了食物中毒性弧菌广谱、快速、特异的同步免疫学检测方法。

The supernatant of the recombinant E. coli by sonication could not only repress the pigment synthesize of Chromobacterium violaceum ATCC 12472, but also could attenuate the intensity of bioluminescence of V. harveyi VIB391 and V. harveyi BB886 by 68% and 53%, respectively.

将表达的重组菌体经超声波破碎后,取其上清液作用于紫色色杆菌ATCC 12472,使其受密度感应系统控制的紫色色素的合成受到抑制;又用上清液分别作用于哈维氏弧菌的发光菌株VIB391和BB886,可使其发光强度分别减弱68%和53%。

Objective To express the N-terminal fragment of human mast cell chymase in E.coli and prepare its polyclonal antibody from mouse.

目的:在大肠杆菌中表达人肥大细胞类糜蛋白酶N端片段,并制备其鼠源性多克隆抗体。

YIp5 plasmid which is replicable in E. coli but not in yeast is served as clonic carrier and ARS sequence in total DNA of rice cloned by air gun experiment.

以能在大肠杆菌复制,但不能在酵母菌中复制的YIp5质粒作为克隆载体,鸟枪法克隆了水稻总DNA中的ARS顺序。

The commonest organisms isolated in patients with SBP include Escherichia coli, gram positive cocci and enterococci.

在SBP患者分离出的最常见的微生物包括大肠埃希杆菌、革兰阳性球菌和肠球菌。

With amikacin highly sensitive to the isolated strain of Escherichia coli as control, the cure and preventing protective test were made on colibacillosis in broilers infected artificially to evaluate its curative effect, Both Chinese herbal compound prescription and amikacin could control the clinical symptoms of broilers infected artificially by E.

方法]根据中兽医理论自拟中药复方制剂,以对分离株大肠杆菌敏感性较高的丁胺卡那霉素作为对照,对人工感染的肉仔鸡大肠杆菌病进行治疗和预防保护性试验,对其疗效进行评价。

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I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。