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The molecular packing in the crystal suggests the physiological form of the mitochondrial SQR is most likely to be a monomer, unlike that for E. coli which is believed to be a trimer.

此外,通过分析该复合物在晶体中的排列,我们推测它在线粒体内膜上是以单体形式发挥功能的,这一点与原核生物 SQR 的二体/三体化现象不同。

The results showed that its solution containing available chlorine 160 mg/L with a 8 mincontact time killed ≥99.97% of Escherichia coli and Staphylococcus aureus on cloth strips,which was higher than the killing rate produced by chlorinated trisodium phosphate alone(<99.94%).

结果,以其含有效氯160 mg/L溶液作用8 min,对布片上大肠杆菌、金黄色葡萄球菌的杀灭率≥99.97%,好于单纯氯化磷酸三钠(〈99.94%)。

The ferment product of strains JF and Al with a broad spectrum of antimicrobial activity, against one or more sensitive microbes, such as Staphylococcus epidermidis, Enterobacter aerogenes, Escherichia coli and Typhoid bacillus etc, were screened out using the filter paper assay, strain C6 have inhibiting effects on VSMC proliferation.

结果:从槲寄生中分离到JF、A1、G6等11株内生真菌;G6对牛主动脉平滑肌细胞增殖有抑制作用;JF、A1发酵产物具有较广语的抗菌活性,对革兰氏阳性细菌如表皮葡萄球菌,革兰氏阴性细菌如产气肠杆菌、大肠杆菌、伤寒杆菌等均具有较强的抑制作用。

Methods Strains of E. coli, Pseudomonas aeruginosa and Enterococcus faecalis were added in the model biles containing diffenent concentration of unconjugated bilirubin. Observed the change of cholesterol nucleation time.

大肠杆菌、铜绿假单胞菌和粪肠球菌分别加入含有不同浓度游离胆红素的模拟胆汁中,观察胆汁中胆固醇晶体形成时间的变化。

Coli. The enzyme can charge valine with AMP, but hydrolyzes the valyl-adenylate when tRNAIle is added.

这个酶可以便缬氨酸与AMP反应,但当tRNAIIe加入时,它会水解缬氨-腺苷酸。

Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区,克隆到pUCm-T Vector,测定核苷酸序列;再将基因编码区克隆到原核表达载体PET-28a,构建含Mn-SOD基因的重组表达质粒,转化到大肠杆菌BL21中进行IPTG诱导表达。

The invention discloses a pyruvate oxidase gene derived from viridian aerococcus, recombinant expression plasmid constructed by the gene and an engineering strain of escherichia coli gene transformed by the recombinant expression plasmid.

本发明公开了一种绿色气球菌来源的丙酮酸氧化酶基因AvPyOD,该基因所构建的重组表达质粒,以及该重组表达质粒转化的大肠杆菌基因工程菌株。

Objective: To study the distribution of the virulence factor and its relationship with the antibiotic resistance of the pyelonephritic E.coli.

目的:探讨致肾盂肾炎大肠杆菌毒力因子的分布及其与耐药性的关系。

Coli; and to investigate the activities of the protein in vive or vitro.

3研究该蛋白在哮喘等Ⅰ型超敏反应的炎症过程中所具有的一系列免疫学生物活性。

With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.

通过对培养条件的不断优化,重组葡激酶-水蛭素融合蛋白在大肠杆菌BL21(DE3)里得到了高效表达,菌体密度最终达到115g/L以上,可溶性重组融合蛋白占菌体总蛋白的30%以上,含量约为1.1~1.2g/L.5L发酵罐的发酵工艺参数在40L发酵罐中进行了放大培养,结果表明该工艺能有效的放大,可适用于工业生产。

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The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。

Its advantage is that it can be used in smaller units.

其优点在于可以在较小的单位中应用。