查询词典 coli

Pronucleus expression recombinant pMAL-C2X-HBV-X is constructed successfully and with the IPTG induction, the recombinant pMAL-C2X-HBV-X can express the X protein in E. coli JM109, which lays the foundation fur the HBV X protein purification and its biological study.

成功构建了HBV X基因原核表达重组体pMAL-C2X-HBV X；在IPTG的诱导下，该重组体在大肠杆菌中可表达X融合蛋白，为进一步纯化HBV X蛋白和研究其生物学作用奠定了基础。

Coli lpdA mutant has the potential to produce pyruvic acid from xylose and mannose.

这些结果对构建以大肠杆菌为母体的模式"细胞工厂"有参考价值。

As a new recombination system, Red recombination has been widely used in E.coli gene knockout.

Red重组作为一种新的重组系统已经被广泛用于大肠杆菌的基因敲除、基因替换。

How to refold proteins at high concentration with high yield is one of critical techniques of recombinant proteins production.

coli中易聚集形成不溶性、无活性的包涵体，如何对包涵体蛋白进行高浓度、高收率的复性，是重组蛋白质生产的关键性技术之一，且关系到基因工程药物的产业化。

Methods Split the recombinant E. coli for expression of rhOP-1 by ultrasomication in ice bath and extract the inclusion body. Dissolve the inclusion body with 8mol/L urea, purify by SP-Sepharose chromatography, refold by gradient dialysis.

将表达rhOP-1的大肠杆菌菌体在冰浴下超声裂解，分离提取包涵体，用8mol/L尿素溶解，纯化后进行梯度透析复性。

HCV NS3C/NS4 protein fused with E. coli thioredoxin was expressed insolubly although thioredoxin is a highly soluble fusion partner. NS5B-dc21 protein was soluble, which is a truncated version of NS5B, while NS5AB protein was insoluble, which needed resolubilization and refold procedures.

HCV NS3C/NS4蛋白是以硫氧还蛋白融合表达的方式获得的，尽管硫氧还蛋白有很强的助溶作用，该蛋白依然不可溶形式存在；NS5B—dc21蛋白通过C末端疏水序列的删除，获得了可溶表达；NS5AB蛋白以不可溶形式表达，需要复性。

Citri had been selected by ribosome display in previous research. In order to express the scFvs with bio-activity efficiently, the genes of the selected scFvs were transformed into Escherichia coli HB2151 for expressing resoluble antibodies. The expression levels of scFvs were detected by dot blotting and Western blotting, and the expressed scFvs were purified by affinity chromatography, and then the activity of the purified scFvs was tested by enzyme-linked immunosorbent assay .

Citri， Xac高亲和力单链抗体(Single chain variable fragment， scFv)GX13、GX44和GX95，为高效表达具有生物活性的单链抗体，本研究将前期筛选的高亲和力单链抗体基因转入大肠杆菌HB2151中进行可溶性表达，点印迹和Western印迹检测可溶性单链抗体的表达水平，亲和层析纯化表达的单链抗体，酶联免疫吸附法检测纯化单链抗体的抗原结合活性。

High-level expression of prochymosin in E. coli: effect of the ribosome binding site.

猪卵透明带-3α基因在大肠杆菌中的融合表达和鉴定。

Escherichia coli A rod-shaped Gramnegative bacterium, some strains of which are normally present in animal intestines, soil, or water.

大肠杆菌：是一种杆状的格兰氏阴性菌，通常存在于动物肠内、水中或土壤中。

Expression of fusion proteins in Escherichia coli Rosetta was detected after induction by IPTG.

在IPTG诱导的条件下，观察融合蛋白在大肠杆菌Rosetta中的表达情况。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。