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Resultsthe lowest spermicidal concentration of ethanol extract of rhynchosia volubilis lour roots on mouse and human sperm were 50mg/ml and 100mg/ml, respectively.the lowest inhibiting concentration of ethanol extract of rhynchosia volubilis lour roots on staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, and pneumobacillus were 1.0,1.0,4.0,4.0 mg/ml,respectively.conclusionthe ethanol extract of rhynchosia volubilis lour roots has spermicidal and antibacterial effect.

结果鹿藿醇提取物对小鼠和人精子最低杀精浓度分别为50和100 mg/ml；对标准菌金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌mic分别为1.0，1.0，4.0，4.0 mg/ml。对临床分离菌金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌mic分别为0.5，2.0，8.0 mg/ml。结论鹿藿醇提取物对小鼠和人精子有一定杀精作用，对临床常见致病菌有比较强的抗菌作用。

Results: The antibacterials experiment showed that the usnic acid material and the SMEDDS had inhibitory action to staphylococcus aureus and pneumococcus, but no inhibitory action to pseudomonas aeruginosa and bacillus coli.

结果 松萝酸原料药及其自微乳制剂对金黄色葡萄球菌和肺炎球菌的生长有抑制作用，对绿脓杆菌和大肠杆菌无效。

The recombinant protein was purified with the different pH. Western blotting testing of the porcine expression of pGEMX POB in E.coli BL21 was positive.

利用非融合表达产品制备抗血清，检测融合表达的重组蛋白，Western-blot为阳性。

Pokeweed antiviral protein belongs to Ribosome-inactive Proteins. PAP has potent antiviral activity against many plant virus. pT1 was used as the template to amplify the deleted mutant PAP gene (N-deleted and N-,C-deIeted) by PCR and then the two genes were cloned into expression vector. Different IPTG-inducible expression vector containing different deleted mutant PAP gene was constructed and transferred into E.coli strain BL21 (DE3)-plysS.

美洲商陆抗病毒蛋白是一类能很好的抑制植物病毒的蛋白，很早就有报道低剂量的商陆叶片粗粉末就能明显的抑制TMV在菜豆叶片上局部枯斑的形成，PAP缓释剂也已用于病毒病的防治工作，但美洲商陆抗病毒蛋白属于核糖体失活蛋白，它对生长的细胞会有一定的毒性。

PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶，切下表达的目的蛋白，冰浴研磨后用磷酸缓冲液溶解，再加入等体积的福氏不完全佐剂，乳化完全后，免疫家兔，制备抗PAP蛋白的多克隆抗体，间接ELISA法测定所制备的抗血清的效价为1：1000。

The genetic basis for FAP lies primarily in the mutation of the adenomatous polyposis coli gene.

大部份的家族性大肠瘜肉症患者会有APC基因的突变。

Methods HIV-1 env gp41 gene of BH10 strain (nt6 977-7 497) was constructed into expressing vector pBV221 and expressed in E. Coli HB101. The expressed proteins were purified on 15% SDS-PAGE, the specific protein gel was cut down, transferred onto nitrocellulose membrane and stained with ponceau for 10 minutes.

表达产物通过15%SDS-聚丙烯酰胺凝胶电泳初步分离纯化，根据RF值，切下含特异蛋白的胶带，以Western blot法转移在硝酸纤维素膜上；免疫血清法检测合格者制备的抗原检测条带，经国家标准参比血清检测。

Indirect enzyme-linked immunosorbent assay for detecting antibodies against avian influenza virus was developed by using expressed full length nucleoprotein of H9N2 AIV in E.coli.263 chicken serum samples(including 243 clinical serum samples and 20 positive serum samples from H9N2 AIV vaccinated chicks) were detected by NP-ELISA,agar gel precipitin test,and hemagglutination inhibition.

以大肠杆菌系统表达的H9N2亚型禽流感病毒核蛋白为抗原，建立了禽流感间接酶联免疫吸附试验抗体检测技术。

And the difference between this two prokaryotic expression systems of curcin2 was compared.

并分别在E.coli Bl21和M15诱导表达，以比较curcin2基因的这两种原核表达系统的差异。

The gene was cloned into a prokaryotic expression vector and high efficiency expression was realized in E.coli.

结果 克隆了中国人肥胖基因，其cDNA顺序与已报道的白种人的序列完全一致，并成功在大肠杆菌中获得表达。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。